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− | <h1><h1 style="font-family:"Helvetica;" "font size=30;" "color: | + | <h1><h1 style="font-family:"Helvetica;" "font size=30;" "color: #434343">BIOBRICK CONSTRUCTION </h1> |
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− | + | Our team was successful in synthesizing a biobrick, as demonstrated by the expression of the Chloro gene when the construct was transformed into E. Coli cultures. Successful growth signified integration of the PETase gene into the plasmid, since the plasmid backbone used (PSIBC3) contained terminator sequences that prevented the expression of Chloro unless the linear backbone was converted into a circular plasmid. The expression of Chloro resistance in E. Coli would thus only have been possible if the assembly of our biobrick had been successful. | |
− | + | </p> | |
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Our team was successful in synthesizing a biobrick, as demonstrated by the expression of the Chloro gene when the construct was transformed into <i> E. Coli </i> cultures. Successful growth signified integration of the PETase gene into the plasmid. | Our team was successful in synthesizing a biobrick, as demonstrated by the expression of the Chloro gene when the construct was transformed into <i> E. Coli </i> cultures. Successful growth signified integration of the PETase gene into the plasmid. | ||
Revision as of 14:35, 18 October 2016
Results
>
BIOBRICK CONSTRUCTION
Our team was successful in synthesizing a biobrick, as demonstrated by the expression of the Chloro gene when the construct was transformed into E. Coli cultures. Successful growth signified integration of the PETase gene into the plasmid, since the plasmid backbone used (PSIBC3) contained terminator sequences that prevented the expression of Chloro unless the linear backbone was converted into a circular plasmid. The expression of Chloro resistance in E. Coli would thus only have been possible if the assembly of our biobrick had been successful.
Table 1: Change in Average Mass of PET Film (g) per Promoter
Day 0 | Day 7 | Day 13 | |
---|---|---|---|
Strong | 0.05175 | 0.05175 | 0.05175 |
Moderately-Strong | 0.071 | 0.071 | 0.07125 |
Weak | 0.06675 | 0.06825 | 0.06725 |
Graph 1: Average PET Mass Sample over Time