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Revision as of 01:58, 19 October 2016

Collaborations

iGEM Concordia

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We collaborated with iGEM Concordia to validate the functionality of our constructs BBa_K2048001 and BBa_K2048002. We have shipped our DNA samples to Concordia iGem on September 2016. Following protocol was executed by iGem Concordia for the preliminary results:

  1. Transformed E.Coli DH5a cells with BBa_K2048001 and BBa_K2048002 separately.
  2. For control groups untransformed E.Coli DH5a cells were used.
  3. 10uM and 100uM gold(III) metal ions were added to the control and experimental groups along with XGal
  4. Blue color change was observed.

Results:

After the 2-3 hours, visible blue color was observed in 10uM and 100uM tubes containing E.Coli BBa_K2048001. Faint blue color was also observed in 10uM and 100uM tubes containing E.Coli BBa_K2048002.

After 24h, tubes with BBa_K2048002 device also showed dark blue color. In addition, BBa_K2048002 tubes had a darker visible blue compared to BBa_K2048001.

Concordia iGEM teams results further validated the functionality of our devices BBa_K2048001 and BBa_K2048002. Moreover, they proved the expression levels can be observed at 100uM gold(III) levels in presence of XGal.

Wiki Generator Collaboration

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