Difference between revisions of "Team:NYMU-Taipei/Demonstrate"

Line 53: Line 53:
 
<h2 style="margin-top:20px; margin-bottom:10px;">Overview</h2><hr />
 
<h2 style="margin-top:20px; margin-bottom:10px;">Overview</h2><hr />
  
<p style="font-size:16px;">Current method of applying entomogenous fungal pesticides is to suspend fungal spores into a solution and introduced through large-scaled spraying. However, with genetically engineered fungi, the contemporary biopesticide deployment method risk damaging the eco-system from the residual fungi. Our project is divided into two main focuses. While our team members in wet lab aim to design a killing switch circuit to decrease the environmental risk of genetically engineered fungus from within the organism. Our prototype team try to avoid the risk of large-scaled pesticide spraying by designing and building an Integrated Orchard Safeguard system centered around our prototype bait trap. </p>
+
<p style="font-size:16px;">Current method of applying entomogenous fungal pesticides large-scaled spraying fungal spore solution. However, deployment of genetically engineered fungal pesticides using contemporary methods puts the local eco-system at risk of disruption by the residual fungi.</p>
  
<p style="font-size:16px;">In modern bait traps, the bait only attract B. dorsalis. However, it is female flies that is responsible for the species' high reproductiveness and destructiveness. This is why we designed the bait trap to attract, infect, and release male flies to allow M. anisopliae infection of female flies via copulation. The IR counter, another feature of our prototype, record and provide information including pest population size and meteorological data, such as temperature and humidity. Furthermore, farmers can monitor their orchards with a smartphone app that receives data from the IR counter.</p>
+
<p style="font-size:16px;">Our project is divided into two main focuses to neutralize these risks. While our team members in wet lab aim to design a killing switch circuit to decrease the environmental risk of genetically engineered fungus from within the organism. Our prototype team attempt introduce an alternative to large-scaled spraying of genetically modified biopesticide by designing and building an Integrated Orchard Safeguard system centered around our prototype bait trap.</p>
  
<p style="font-size:16px;">We present the Integrated Orchard Safeguard, a informative defense system that links our <font color="#FF0000"><b>bait trap, IR counter, and smartphone app.</b></font></p>
+
<p style="font-size:16px;">In modern bait traps, the bait only attract male B. dorsalis. However, it is the females that are responsible for the species' high reproductiveness and destructiveness. This is why we designed the bait trap to attract, infect, and release male flies to allow M. anisopliae infection to spread to the females via copulation with infected males. The IR counter, another feature of our prototype, record and provide information including pest population size and meteorological data, such as temperature and humidity. Furthermore, farmers can monitor their orchards with our smartphone app that receives data from the IR counter.</p>
 +
 
 +
<p style="font-size:16px;">We present the Integrated Orchard Safeguard, an information network system that connects our <font color="#FF0000"><b>bait trap, IR counter and smartphone app together.</b></font></p>
  
 
<div align="center">
 
<div align="center">
Line 66: Line 68:
 
<div class="fund">
 
<div class="fund">
  
<h2 style="margin-top:30px; margin-bottom:10px;">Bait Trap</h2><hr />
+
<h2 style="margin-top:30px; margin-bottom:10px;">New Generation Bait Trap</h2><hr />
  
<p style="font-size:16px;">After the bait trap receives the population size of oriental fruit fly from the IR counter, it will then <font color="#FF0000"><b>automatically regulate the frequency </b></font>of the spray of fungal spore solution and the opening duration of the door.</p>
+
<p style="font-size:16px;">After the bait trap receives the population size information of <i>B. dorsalis</i> from the IR counter, it will then <font color="#FF0000"><b>automatically regulate the frequency </b></font>of the spray of fungal spore solution and the opening duration of the door.</p>
  
 
<div class="imageimage">
 
<div class="imageimage">
Line 105: Line 107:
 
<img src="https://static.igem.org/mediawiki/2016/a/a9/T-NYMU-Taipei-photo-floor_1.png" width="100%" /></div>
 
<img src="https://static.igem.org/mediawiki/2016/a/a9/T-NYMU-Taipei-photo-floor_1.png" width="100%" /></div>
 
<div style="position:relative; width:48%;">
 
<div style="position:relative; width:48%;">
<p style="font-size:16px;">Since flying up is one of the behaviors of B. dorsalis, we set the <font color="#FF0000"><b>entrance</b></font> at the bottom of the trap to hinder the escape of trapped flies. The grower can pull out the drawer to refill the <font color="#FF0000"><b>methyl eugenol(a pheromone precursor)</b></font> to maintain the trap's attraction to male flies. The <font color="#FF0000"><b>atomization device</b></font> in the drawer sprays fungal spore solution to infect the trapped male flies.</p></div>
+
<p style="font-size:16px;">Since flying up is one of the behaviors of <i>B. dorsalis</i>, we set the <font color="#FF0000"><b>entrance</b></font> at the bottom of the trap to hinder the escape of trapped flies. The grower can pull out the drawer to refill the <font color="#FF0000"><b>methyl eugenol(a pheromone precursor)</b></font> to maintain the trap's attraction to male flies. The <font color="#FF0000"><b>atomization device</b></font> in the drawer sprays fungal spore solution to infect the trapped male flies.</p></div>
 
</div>
 
</div>
  
Line 116: Line 118:
 
<h4 style="margin-top:30px; margin-bottom:10px;">1. Specificity - Methyl Eugenol</h4>
 
<h4 style="margin-top:30px; margin-bottom:10px;">1. Specificity - Methyl Eugenol</h4>
 
<p style="font-size:16px;">
 
<p style="font-size:16px;">
<font color="#FF0000"><b>Methyl Eugenol</b></font> is the <font color="#FF0000"><b>pheromone precursor</b></font> of B. dorsalis. It only attracts male flies. The male flies will fly away once they  have consumed enough Methyl Eugenol.
+
<font color="#FF0000"><b>Methyl Eugenol</b></font> is the <font color="#FF0000"><b>pheromone precursor</b></font> of <i>B. dorsalis</i>. It only attracts male flies. The male flies will fly away once they  have consumed enough Methyl Eugenol.
 
</p>
 
</p>
<h4 style="margin-top:30px; margin-bottom:10px;">2. Target main Problem – Female B. dorsalis</h4>
+
<h4 style="margin-top:30px; margin-bottom:10px;">2. Target main Problem – Female <i>B. dorsalis</i></h4>
 
<p style="font-size:16px;">
 
<p style="font-size:16px;">
<font color="#FF0000"><b>female B. dorsalis</b></font> poses more threat than males.  
+
<font color="#FF0000"><b>female <i>B. dorsalis</i></b></font> poses more threat than males.  
 
</p>
 
</p>
 
<h4 style="margin-top:30px; margin-bottom:10px;">3. Regulation – IR Counter, Trap, Phone App</h4>
 
<h4 style="margin-top:30px; margin-bottom:10px;">3. Regulation – IR Counter, Trap, Phone App</h4>
Line 171: Line 173:
  
 
<div style="position:relative; width:48%;">
 
<div style="position:relative; width:48%;">
<p style="font-size:16px;">To <font color="#FF0000"><b>improve the accuracy</b></font> of counter, we tried to find the proper tunnel diameter allows only <font color="#FF0000"><b>one-by-one passage of B. dorsalis</b></font>. We prepared different size of straws to connect two centrifuge tubes. One contained male B. dorsalis, and the other contained the bait, methyl eugenol. </p></div>
+
<p style="font-size:16px;">To <font color="#FF0000"><b>improve the accuracy</b></font> of counter, we tried to find the proper tunnel diameter allows only <font color="#FF0000"><b>one-by-one passage of <i>B. dorsalis</i></b></font>. We prepared different size of straws to connect two centrifuge tubes. One contained male <i>B. dorsalis</>, and the other contained the bait, methyl eugenol. </p></div>
  
 
</div>
 
</div>
Line 210: Line 212:
 
<h2 style="margin-top:30px; margin-bottom:10px;">Modeling Efficiency</h2><hr>
 
<h2 style="margin-top:30px; margin-bottom:10px;">Modeling Efficiency</h2><hr>
  
<p style="font-size:16px;">A population size model of B. dorsalis was constructed to simulate a comparison of B. dorsalis population size between an IOS-protected orchard and a normal orchard. The results show a significant decrease of 35% in B. dorsalis population in an IOS-protected orchard compared to one without IOS.
+
<p style="font-size:16px;">A population size model of <i>B. dorsalis</i> was constructed to simulate a comparison of <i>B. dorsalis </i>population size between an IOS-protected orchard and a normal orchard. The results show a significant decrease of 35% in <i>B. dorsalis</i> population in an IOS-protected orchard compared to one without IOS.
 
<br>
 
<br>
 
<a href="https://2016.igem.org/Team:NYMU-Taipei/Project-Model">Link to modeling</a>
 
<a href="https://2016.igem.org/Team:NYMU-Taipei/Project-Model">Link to modeling</a>
Line 286: Line 288:
  
 
<div class="fund">
 
<div class="fund">
<p style="font-size:16px;">It is estimated that orchard without any B. dorsalis treatment may incur lost up to 30% of fruit(1). A few calculation was done based on the data from government, and it shows that the saving of fruit value due to our IOS is much greater than the cost of IOS, suggesting our IOS is really worthwhile to be implemented.</p><br />
+
<p style="font-size:16px;">It is estimated that orchard without any <i>B. dorsalis</i> treatment may incur lost up to 30% of fruit(1). A few calculation was done based on the data from government, and it shows that the saving of fruit value due to our IOS is much greater than the cost of IOS, suggesting our IOS is really worthwhile to be implemented.</p><br />
  
 
<img src="https://static.igem.org/mediawiki/2016/d/d3/T-NYMU-Taipei-photo-protype_image005.png" width="90%" />
 
<img src="https://static.igem.org/mediawiki/2016/d/d3/T-NYMU-Taipei-photo-protype_image005.png" width="90%" />
Line 319: Line 321:
 
<p style="font-size:16px;">Current method of applying entomogenous fungal pesticides is to suspend fungal spores into a solution and introduced through large-scaled spraying. However, with genetically engineered fungi, the contemporary biopesticide deployment method risk damaging the eco-system from the residual fungi. Our project is divided into two main focuses. While our team members in wet lab aim to design a killing switch circuit to decrease the environmental risk of genetically engineered fungus from within the organism. Our prototype team try to avoid the risk of large-scaled pesticide spraying by designing and building an Integrated Orchard Safeguard system centered around our prototype bait trap.</p>
 
<p style="font-size:16px;">Current method of applying entomogenous fungal pesticides is to suspend fungal spores into a solution and introduced through large-scaled spraying. However, with genetically engineered fungi, the contemporary biopesticide deployment method risk damaging the eco-system from the residual fungi. Our project is divided into two main focuses. While our team members in wet lab aim to design a killing switch circuit to decrease the environmental risk of genetically engineered fungus from within the organism. Our prototype team try to avoid the risk of large-scaled pesticide spraying by designing and building an Integrated Orchard Safeguard system centered around our prototype bait trap.</p>
  
<p style="font-size:16px;">In modern bait traps, the bait only attract B. dorsalis. However, it is female flies that is responsible for the species' high reproductiveness and destructiveness. This is why we designed the bait trap to attract, infect, and release male flies to allow M. anisopliae infection of female flies via copulation. The IR counter, another feature of our prototype, record and provide information including pest population size and meteorological data, such as temperature and humidity. Furthermore, farmers can monitor their orchards with a smartphone app that receives data from the IR counter.</p>
+
<p style="font-size:16px;">In modern bait traps, the bait only attract <i>B. dorsalis</i>. However, it is female flies that is responsible for the species' high reproductiveness and destructiveness. This is why we designed the bait trap to attract, infect, and release male flies to allow M. anisopliae infection of female flies via copulation. The IR counter, another feature of our prototype, record and provide information including pest population size and meteorological data, such as temperature and humidity. Furthermore, farmers can monitor their orchards with a smartphone app that receives data from the IR counter.</p>
  
 
<p style="font-size:16px;">We present the Integrated Orchard Safeguard, a informative defense system that links our <font color="#FF0000"><b>bait trap, IR counter, and smartphone app.</b></font></p>
 
<p style="font-size:16px;">We present the Integrated Orchard Safeguard, a informative defense system that links our <font color="#FF0000"><b>bait trap, IR counter, and smartphone app.</b></font></p>
Line 360: Line 362:
 
<img src="https://static.igem.org/mediawiki/2016/a/a9/T-NYMU-Taipei-photo-floor_1.png" width="100%" />
 
<img src="https://static.igem.org/mediawiki/2016/a/a9/T-NYMU-Taipei-photo-floor_1.png" width="100%" />
  
<p style="font-size:16px;">Since flying up is one of the behaviors of B. dorsalis, we set the <font color="#FF0000"><b>entrance</b></font> at the bottom of the trap to hinder the escape of trapped flies. The grower can pull out the drawer to refill the <font color="#FF0000"><b>methyl eugenol(a pheromone precursor)</b></font> to maintain the trap's attraction to male flies. The <font color="#FF0000"><b>atomization device</b></font> in the drawer sprays fungal spore solution to infect the trapped male flies.</p>
+
<p style="font-size:16px;">Since flying up is one of the behaviors of <i>B. dorsalis</i>, we set the <font color="#FF0000"><b>entrance</b></font> at the bottom of the trap to hinder the escape of trapped flies. The grower can pull out the drawer to refill the <font color="#FF0000"><b>methyl eugenol(a pheromone precursor)</b></font> to maintain the trap's attraction to male flies. The <font color="#FF0000"><b>atomization device</b></font> in the drawer sprays fungal spore solution to infect the trapped male flies.</p>
  
 
</div>
 
</div>
Line 369: Line 371:
  
 
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">1. Specificity - Methyl Eugenol</h4>
 
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">1. Specificity - Methyl Eugenol</h4>
<p style="font-size:16px;"><font color="#FF0000"><b>Methyl Eugenol</b></font> is the <font color="#FF0000"><b>pheromone precursor</b></font> of B. dorsalis. It only attracts male flies. The male flies will fly away once they  have consumed enough Methyl Eugenol.</p>
+
<p style="font-size:16px;"><font color="#FF0000"><b>Methyl Eugenol</b></font> is the <font color="#FF0000"><b>pheromone precursor</b></font> of <i>B. dorsalis</i>. It only attracts male flies. The male flies will fly away once they  have consumed enough Methyl Eugenol.</p>
  
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">2. Target main Problem – Female B. dorsalis</h4>
+
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">2. Target main Problem – Female <i>B. dorsalis</i></h4>
<p style="font-size:16px;"><font color="#FF0000"><b>female B. dorsalis</b></font> poses more threat than males. </p>
+
<p style="font-size:16px;"><font color="#FF0000"><b>female <i>B. dorsalis</i></b></font> poses more threat than males. </p>
  
 
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">3. Regulation – IR Counter, Trap, Phone App</h4>
 
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">3. Regulation – IR Counter, Trap, Phone App</h4>
Line 414: Line 416:
 
<img src="https://static.igem.org/mediawiki/2016/thumb/4/4d/T-NYMU-Taipei-photo-14647197_120300000625973835_1048154200_o.jpg/180px-T-NYMU-Taipei-photo-14647197_120300000625973835_1048154200_o.jpg" width="90%" />
 
<img src="https://static.igem.org/mediawiki/2016/thumb/4/4d/T-NYMU-Taipei-photo-14647197_120300000625973835_1048154200_o.jpg/180px-T-NYMU-Taipei-photo-14647197_120300000625973835_1048154200_o.jpg" width="90%" />
  
<p style="font-size:16px;">To <font color="#FF0000"><b>improve the accuracy</b></font> of counter, we tried to find the proper tunnel diameter allows only <font color="#FF0000"><b>one-by-one passage of B. dorsalis</b></font>. We prepared different size of straws to connect two centrifuge tubes. One contained male B. dorsalis, and the other contained the bait, methyl eugenol. </p>
+
<p style="font-size:16px;">To <font color="#FF0000"><b>improve the accuracy</b></font> of counter, we tried to find the proper tunnel diameter allows only <font color="#FF0000"><b>one-by-one passage of <i>B. dorsalis</i></b></font>. We prepared different size of straws to connect two centrifuge tubes. One contained male <i>B. dorsalis</i>, and the other contained the bait, methyl eugenol. </p>
  
 
</div>
 
</div>
Line 420: Line 422:
 
<div class="fund">
 
<div class="fund">
 
<h3 style="margin-top:30px; margin-bottom:10px;">Result</h3>
 
<h3 style="margin-top:30px; margin-bottom:10px;">Result</h3>
<p style="font-size:16px;">Results indicate that when the tunnel diameter is above <font color="#FF0000"><b>0.8 cm</b></font>, the flies can pass through.</p>
+
<p style="font-size:16px;">The results indicate that when the tunnel diameter is above <font color="#FF0000"><b>0.8 cm</b></font>, the flies can pass through.</p>
  
 
<div class="tablesatisfysp" style="overflow-x:auto;">
 
<div class="tablesatisfysp" style="overflow-x:auto;">
Line 451: Line 453:
 
<div class="fund">
 
<div class="fund">
  
<h2 style="margin-top:30px; margin-bottom:10px;">Modeling Efficiency</h2><hr>
+
<h2 style="margin-top:30px; margin-bottom:10px;">Efficiency Model</h2><hr>
  
 
<p style="font-size:16px;">
 
<p style="font-size:16px;">
A population size model of B. dorsalis was constructed to simulate a comparison of B. dorsalis population size between an IOS-protected orchard and a normal orchard. The results show a significant decrease of 35% in B. dorsalis population in an IOS-protected orchard compared to one without IOS.
+
A population size model of <i>B. dorsalis</i> was constructed to simulate a comparison of <i>B. dorsalis</i> population size between an IOS-protected orchard and a normal orchard. The results show a significant decrease of 35% in <i>B. dorsalis</i> population in an IOS-protected orchard compared to one without IOS.
 
<br>
 
<br>
 
<a href="https://2016.igem.org/Team:NYMU-Taipei/Project-Model">Link to modeling</a>
 
<a href="https://2016.igem.org/Team:NYMU-Taipei/Project-Model">Link to modeling</a>
Line 539: Line 541:
 
<div class="fund">
 
<div class="fund">
  
<p style="font-size:16px;">It is estimated that orchard without any B. dorsalis treatment may incur lost up to 30% of fruit(1). A few calculation was done based on the data from government, and it shows that the saving of fruit value due to our IOS is much greater than the cost of IOS, suggesting our IOS is really worthwhile to be implemented.</p>
+
<p style="font-size:16px;">It is estimated that orchard without any <i>B. dorsalis</i> treatment may incur lost up to 30% of fruit(1). A few calculation was done based on the data from government, and it shows that the saving of fruit value due to our IOS is much greater than the cost of IOS, suggesting our IOS is really worthwhile to be implemented.</p>
  
 
<div align="center">
 
<div align="center">

Revision as of 17:19, 18 October 2016

Prototype

Prototype



Integrated Orchard Safeguard

Overview


Current method of applying entomogenous fungal pesticides large-scaled spraying fungal spore solution. However, deployment of genetically engineered fungal pesticides using contemporary methods puts the local eco-system at risk of disruption by the residual fungi.

Our project is divided into two main focuses to neutralize these risks. While our team members in wet lab aim to design a killing switch circuit to decrease the environmental risk of genetically engineered fungus from within the organism. Our prototype team attempt introduce an alternative to large-scaled spraying of genetically modified biopesticide by designing and building an Integrated Orchard Safeguard system centered around our prototype bait trap.

In modern bait traps, the bait only attract male B. dorsalis. However, it is the females that are responsible for the species' high reproductiveness and destructiveness. This is why we designed the bait trap to attract, infect, and release male flies to allow M. anisopliae infection to spread to the females via copulation with infected males. The IR counter, another feature of our prototype, record and provide information including pest population size and meteorological data, such as temperature and humidity. Furthermore, farmers can monitor their orchards with our smartphone app that receives data from the IR counter.

We present the Integrated Orchard Safeguard, an information network system that connects our bait trap, IR counter and smartphone app together.

New Generation Bait Trap


After the bait trap receives the population size information of B. dorsalis from the IR counter, it will then automatically regulate the frequency of the spray of fungal spore solution and the opening duration of the door.

Design


1. The First Layer – Microcontroller, Power


The Arduino UNO in our design has combined with Appsduino's product, AppsBee Shield and Appsduino Shield. (The web site of Appsduino: http://appsduino.com/)

2. The Second Layer – Motor


We use the string attached to a ULN2003 motor to open and close the door.

3. Drawer – Entrance, Atomization Device


Since flying up is one of the behaviors of B. dorsalis, we set the entrance at the bottom of the trap to hinder the escape of trapped flies. The grower can pull out the drawer to refill the methyl eugenol(a pheromone precursor) to maintain the trap's attraction to male flies. The atomization device in the drawer sprays fungal spore solution to infect the trapped male flies.


Features

1. Specificity - Methyl Eugenol

Methyl Eugenol is the pheromone precursor of B. dorsalis. It only attracts male flies. The male flies will fly away once they have consumed enough Methyl Eugenol.

2. Target main Problem – Female B. dorsalis

female B. dorsalis poses more threat than males.

3. Regulation – IR Counter, Trap, Phone App

Automatically regulation of the spray frequency and door open duration depends on the data collected by the IR counter and phone APP.

4. Eco-Friendly – Recycle

Replacing the indissoluble plastic material (distributed by the government) with recyclable wood chips, which can reduce environmental pollution.

Mechanism


Functionality Display Video


IR Counter & APP


IR counter records and provides real-time information of the orchard : pest group size, meteorological data including temperature and humidity. With a smartphone app, the bait traps are able to inform the grower of these important information.

Design


Functional Test

To improve the accuracy of counter, we tried to find the proper tunnel diameter allows only one-by-one passage of B. dorsalis. We prepared different size of straws to connect two centrifuge tubes. One contained male B. dorsalis, and the other contained the bait, methyl eugenol.

Result

Results indicate that when the tunnel diameter is above 0.8 cm, the flies can pass through.

                                       
Diameter(cm)2.001.751.501.000.800.750.60
outcomeOOOOOHardlyX

Modeling Efficiency


A population size model of B. dorsalis was constructed to simulate a comparison of B. dorsalis population size between an IOS-protected orchard and a normal orchard. The results show a significant decrease of 35% in B. dorsalis population in an IOS-protected orchard compared to one without IOS.
Link to modeling

Population size without IOS



Population size with IOS


Expenditure Analysis


Based on our experimental deployment of counters and traps in an orchard one-hectare in size. The estimated total cost of IOS is listed below:

amount

cost (USD)

trap

4

60

counter

3

30

methyl eugenol

100 mL

50

total cost

90


It is estimated that orchard without any B. dorsalis treatment may incur lost up to 30% of fruit(1). A few calculation was done based on the data from government, and it shows that the saving of fruit value due to our IOS is much greater than the cost of IOS, suggesting our IOS is really worthwhile to be implemented.


(cost of IOS only in low ratio compared to the total profit)



(figure showing the great savings from IOS)



Integrated Orchard Safeguard

Overview


Current method of applying entomogenous fungal pesticides is to suspend fungal spores into a solution and introduced through large-scaled spraying. However, with genetically engineered fungi, the contemporary biopesticide deployment method risk damaging the eco-system from the residual fungi. Our project is divided into two main focuses. While our team members in wet lab aim to design a killing switch circuit to decrease the environmental risk of genetically engineered fungus from within the organism. Our prototype team try to avoid the risk of large-scaled pesticide spraying by designing and building an Integrated Orchard Safeguard system centered around our prototype bait trap.

In modern bait traps, the bait only attract B. dorsalis. However, it is female flies that is responsible for the species' high reproductiveness and destructiveness. This is why we designed the bait trap to attract, infect, and release male flies to allow M. anisopliae infection of female flies via copulation. The IR counter, another feature of our prototype, record and provide information including pest population size and meteorological data, such as temperature and humidity. Furthermore, farmers can monitor their orchards with a smartphone app that receives data from the IR counter.

We present the Integrated Orchard Safeguard, a informative defense system that links our bait trap, IR counter, and smartphone app.

Bait Trap


After the bait trap receives the population size of oriental fruit fly from the IR counter, it will then automatically regulate the frequency of the spray of fungal spore solution and the opening duration of the door.

Design


1. The First Layer – Microcontroller, Power



The Arduino UNO in our design has combined with Appsduino's product, AppsBee Shield and Appsduino Shield. (The web site of Appsduino: http://appsduino.com/)

2. The Second Layer – Motor


We use the string attached to a ULN2003 motor to open and close the door.

3. Drawer – Entrance, Atomization Device


Since flying up is one of the behaviors of B. dorsalis, we set the entrance at the bottom of the trap to hinder the escape of trapped flies. The grower can pull out the drawer to refill the methyl eugenol(a pheromone precursor) to maintain the trap's attraction to male flies. The atomization device in the drawer sprays fungal spore solution to infect the trapped male flies.


Features

1. Specificity - Methyl Eugenol

Methyl Eugenol is the pheromone precursor of B. dorsalis. It only attracts male flies. The male flies will fly away once they have consumed enough Methyl Eugenol.

2. Target main Problem – Female B. dorsalis

female B. dorsalis poses more threat than males.

3. Regulation – IR Counter, Trap, Phone App

Automatically regulation of the spray frequency and door open duration depends on the data collected by the IR counter and phone APP.

4. Eco-Friendly – Recycle

Replacing the indissoluble plastic material (distributed by the government) with recyclable wood chips, which can reduce environmental pollution.

Mechanism


Functionality Display Video


IR Counter & APP


IR counter records and provides real-time information of the orchard : pest group size, meteorological data including temperature and humidity. With a smartphone app, the bait traps are able to inform the grower of these important information.

Design


Functional Test

To improve the accuracy of counter, we tried to find the proper tunnel diameter allows only one-by-one passage of B. dorsalis. We prepared different size of straws to connect two centrifuge tubes. One contained male B. dorsalis, and the other contained the bait, methyl eugenol.

Result

The results indicate that when the tunnel diameter is above 0.8 cm, the flies can pass through.

                                       
Diameter(cm)2.001.751.501.000.800.750.60
outcomeOOOOOHardlyX

Efficiency Model


A population size model of B. dorsalis was constructed to simulate a comparison of B. dorsalis population size between an IOS-protected orchard and a normal orchard. The results show a significant decrease of 35% in B. dorsalis population in an IOS-protected orchard compared to one without IOS.
Link to modeling

Population size without IOS



Population size with IOS



Expenditure Analysis


Based on our experimental deployment of counters and traps in an orchard one-hectare in size. The estimated total cost of IOS is listed below:

amount

cost (USD)

trap

4

60

counter

3

30

methyl eugenol

100 mL

50

total cost

90

It is estimated that orchard without any B. dorsalis treatment may incur lost up to 30% of fruit(1). A few calculation was done based on the data from government, and it shows that the saving of fruit value due to our IOS is much greater than the cost of IOS, suggesting our IOS is really worthwhile to be implemented.

(cost of IOS only in low ratio compared to the total profit)


(figure showing the great savings from IOS)


Reference:


1.http://www.mdais.gov.tw/files/web_articles_files/mdares/2469/631.pdf 2.http://ir.tari.gov.tw:8080/bitstream/345210000/281/1/57_1_5.pdf