Difference between revisions of "Team:LMU-TUM Munich/Proof"
(→In the Micromanipulator) |
(→In the Micromanipulator) |
||
| Line 55: | Line 55: | ||
<source src="https://static.igem.org/mediawiki/2016/e/e0/Muc16_Mikromanipulator_negK.mov" type="video/mp4"> | <source src="https://static.igem.org/mediawiki/2016/e/e0/Muc16_Mikromanipulator_negK.mov" type="video/mp4"> | ||
Your browser does not support this video player.</video> | Your browser does not support this video player.</video> | ||
| − | <video width=49% style="float:right | + | <video width=49% style="float:right; margin-top : 15px"; margin-bottom : 15px; controls preload:"auto"> |
<source src="https://static.igem.org/mediawiki/2016/d/d6/Muc16_Mikromanipulator_Poly.mov" type="video/mp4"> | <source src="https://static.igem.org/mediawiki/2016/d/d6/Muc16_Mikromanipulator_Poly.mov" type="video/mp4"> | ||
Your browser does not support this video player.</video></p></html><br><br><br><br><br><br><br><br><br> | Your browser does not support this video player.</video></p></html><br><br><br><br><br><br><br><br><br> | ||
Revision as of 21:34, 18 October 2016
Wichtiger Kommentar: Polymerization wird Proof-of-Principle page.
Introduction to polymerization
Design: Why we chose the Avidin & Biotin affinity pair
- Vergleich mit anderen Polymerisationsmöglichkeiten die wir diskutiert hatten
- Tabelle mit Literaturwerten zu Assoziationsraten
| Affinity pair | Kind of interaction | Dissociation constant | Association rate | Comment |
| Avidin - Biotin | non covalent | 10-15M [1] | 55 440 | |
| SpyCatcher - SpyTag | covalent | not applicable | ||
| StrepTactin - Strep-tag II |
Multivalency and avidity as major principles for polymerization
- Strukturmodeling
Different polymers possible with biotINK polymerization
Since the foundation of our polymerization reaction is the interaction of Streptavidin and its binding partner Biotin (Referenz) we used biotinylated polymers to convery the polymerization between the cells.
- Mesh-Bilder von Luisa
Results: Polymerization experiments
In the Micromanipulator
References
- ↑ Green, N. M. (1963). Avidin. 1. The use of [14C] biotin for kinetic studies and for assay. Biochemical Journal, 89(3), 585.
