Difference between revisions of "Team:Dundee Schools/Experiments"

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<h4 id="page_name"> Experiments </h4>
 
<h4 id="page_name"> Experiments </h4>
  
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<table>
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<tr>
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  <th colspan="2">Standard PCR reaction (final volume =50 μl)
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</tr>
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<tr>
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  <th>Component</th>
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  <th>Volume (μl)</th>
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</tr>
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<tr>
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  <td>5x Herculase buffer</td>
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  <td>10</td>
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</tr>
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<tr>
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  <td>100 μM forward primer</td>
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  <td>0.5</td>
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</tr>
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<tr>
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  <td>100 μM reverse primer</td>
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  <td>0.5></td>
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</tr>
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<tr>
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  <td>10 mM dNTPs</td>
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  <td>0.5</td>
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</tr>
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<tr>
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  <td>DMSO</td>
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  <td>2.5</td>
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</tr>
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<tr>
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  <td>H<sub>2</sub>O</td>
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  <td>34.5</td>
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</tr>
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<tr>
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  <td>gDNA</td>
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  <td>1</td>
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</tr>
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<tr>
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  <td>Herculase II</td>
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  <td>0.5</td>
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</tr>
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</table>
  
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</body>
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<script>
 
<script>
  

Revision as of 16:52, 19 October 2016

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Dundee Schools

Experiments

Standard PCR reaction (final volume =50 μl)
Component Volume (μl)
5x Herculase buffer 10
100 μM forward primer 0.5
100 μM reverse primer 0.5>
10 mM dNTPs 0.5
DMSO 2.5
H2O 34.5
gDNA 1
Herculase II 0.5