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− | + | <br> After much deliberation, we finally decided to use the Bba_ P10500 Phytobrick Universal Acceptor Vector as our destination vector for all our (level 0) MoClo compatible DNA parts. The order for the synthesis of DNA gblocks for our parts was placed through IDT. <br> | |
+ | <br> Team MoClo: Finished Primer design for mutagenesis & start cloning plasmids for template<br> | ||
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+ | <br> Our first MoClo assembly was finally performed using DNA parts found in the CIDAR MoClo kit. However, very few colonies were obtained and therefore our MoClo reaction mixture and conditions were adapted. <br> | ||
+ | <br> Team MoClo: Mutagenesis & screening of illegal removal sites for Rhodococcus vectors. Mut2 and Mut3 showed a good result.<br> | ||
+ | <img src=”https://static.igem.org/mediawiki/2016/5/56/T--Edinburgh_OG--Matin_Notebook_Week6_Mut.png” width=”100%”> | ||
+ | </p> | ||
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Revision as of 07:15, 19 October 2016
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