Difference between revisions of "Team:ASIJ Tokyo/Parts"

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             <li><a href="https://2016.igem.org/ASIJProjectDescription">Project Description + Abstract</a></li>
 
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         <li><a href="https://2016.igem.org/Team:ASIJ_Tokyo/Safety">Safety</a></li>
 
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         <li><a href="https://2016.igem.org/Team:ASIJ_Tokyo/Parts">Parts</a></li>
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Revision as of 11:30, 19 October 2016

The BIG TEMPLATE : RESPONSIVE and FREE

Parts


Andersen Promoters
  • Relative Strength: weak (0.33) BBa_J23110
    • Selected as a relatively weak promoter against which to compare the expression of the PETase construct
  • Relative Strength: moderate (0.58) BBa_J23111
    • Selected as a relatively moderately strong promoter against which to compare the expression of the PETase construct
  • Relative Strength: strong (1) BBa_J23100
    • Selected as a relatively strong promoter against which to compare the expression of the PETase construct
  • N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of e. Coli
  • C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression
  • PETase sequence: gene that coded for the PET hydrolase
  • Plasmid backbone: the pSB1C3 backbone is recommended
  • High efficiency e. Coli cells: hosted the PETase constructs