Difference between revisions of "Team:DTU-Denmark/Safety"
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| − | + | <!-- Edited by Isabella 7-9-16 14:30 in sidebar - remove xs-hidden from sidebar --> | |
| + | <!-- Edited by Isabella 26-9-16-14:22 fix white space above picture, do not edit the spaces between things this create unintentional space--> | ||
| + | <title>Bootstrap Example</title> | ||
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| − | + | <div class="col-md-12 thumbnail" style="background-image:url(https://static.igem.org/mediawiki/2016/5/56/T--DTU-Denmark--project-header.jpg)"> <!-- EDIT style url (this should lead to an image) --> | |
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| − | . | + | <div class="col-md-5 col-sm-5 col-xs-12 title"> <!-- the approximate max number of characters ~ 400 --> <!-- EDIT --> |
| − | + | <h1>Safety<p class="lead">leader under the title, short introduction. Ubique moderatius efficiantur eum et, dico oporteat recusabo ius cu, pro id modus sadipscing. Maluisset patrioque eum ad, mel eius doctus accommodare eu, minimum deleniti repudiandae mel ea. Noster nostrud diceret sea no. Eos an nullam molestiae signiferumque, vel ne laudem ignota oblique. Duo te luptatum percipitur signiferumque, at dicunt iriure dolorem his.</p></h1> | |
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| − | + | <div class="col-md-2 col-sm-2 hidden-xs space"></div> | |
| − | / | + | <div class="col-md-5 col-sm-5 hidden-xs intro"> <!-- will be hidden on phones, duplicate the text to blockquote down below first section header, to show it there, when it dissapear--> |
| − | + | <blockquote class="blockquote-reverse"> <!-- EDIT --> | |
| − | + | <p>Safety is something that happens between your ears, not something you hold in your hands</p> | |
| − | + | <small>Jeff Cooper<cite title="Source Title"></cite></small> | |
| − | + | </blockquote> | |
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| + | </div> | ||
| + | </div> | ||
| + | </div> <!-- /row --> | ||
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| − | + | <div class="row"> <!--Must sorround both content and sidebar--> | |
| − | + | <div class="col-md-9 col-sm-10 colLeft"> <!-- LEFT --> | |
| − | + | <div><a class="anchor" id="section-1"></a> | |
| − | + | <h2 class="h2">Precautionary work</h2> | |
| + | |||
| + | <blockquote class="visible-xs"> <!-- quote from masterhead duplicate --> | ||
| + | <p>Safety is something that happens between your ears, not something you hold in your hands</p> | ||
| + | <small>Jeff Cooper<cite title="Source Title"></cite></small> | ||
| + | </blockquote> | ||
| + | |||
| + | <p>To minimize the risks while working on the iGEM project we choose the chassis organism Yarrowia lipolytica which is generally regarded as safe (GRAS) and non-pathogenic to humans. Aside from that we worked with the Escherichia coli DH5 alpha laboratory strain which does not pose a threat to healthy humans. We also did some growth experiment with Saccharomyces cerevisiae, another GRAS organism. </p> | ||
| − | + | <p>In our choice of genetic material we considered the risks the origin organism could pose. We only used genes from safe sources such as Xanthophyllomyces dendrorhous, Renilla reinformis and the human gene for pre-insulin codon optimised with our own codon-optimization tool for Y. lipolityca.</p> | |
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| − | + | <div><a class="anchor" id="section-2"></a> | |
| − | + | <h2 class="h2">Laboratory safety measures</h2> | |
| − | + | <p>All work with genetically modified organisms (GMOs) were performed in class 1 GMO laboratories following the required procedures. This include amongst others wearing laboratory coats, gloves and goggles, disinfecting surfaces, tools etc. with ethanol and autoclaving all vaste, used tools and discarded cultures. We used laf-benches whenever possible to avoid the fire hazard of gas flames when performing sterile work. The Hamilton robot used as fermentation platform was decontaminated before and after each use with UV light.</p> | |
| − | + | <p>In our work with hazardous substances we took extra safety measures and worked with utmost caution. We used laf-benches and powerful suction in the experiments performed with 2-Mercaptoethanol (BME). SYBR-Safe was always handled with gloves and caution. Containers with liquid nitrogen were only opened and cooling conducted in well ventilated rooms. | |
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| − | + | <li><a href="#section-1">Precautionary work</a></li> | |
| − | + | <li><a href="#section-2">Laboratory safety measures</a></li> | |
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Revision as of 11:38, 19 October 2016
Safety
leader under the title, short introduction. Ubique moderatius efficiantur eum et, dico oporteat recusabo ius cu, pro id modus sadipscing. Maluisset patrioque eum ad, mel eius doctus accommodare eu, minimum deleniti repudiandae mel ea. Noster nostrud diceret sea no. Eos an nullam molestiae signiferumque, vel ne laudem ignota oblique. Duo te luptatum percipitur signiferumque, at dicunt iriure dolorem his.
Precautionary work
Safety is something that happens between your ears, not something you hold in your hands
Jeff Cooper
To minimize the risks while working on the iGEM project we choose the chassis organism Yarrowia lipolytica which is generally regarded as safe (GRAS) and non-pathogenic to humans. Aside from that we worked with the Escherichia coli DH5 alpha laboratory strain which does not pose a threat to healthy humans. We also did some growth experiment with Saccharomyces cerevisiae, another GRAS organism.
In our choice of genetic material we considered the risks the origin organism could pose. We only used genes from safe sources such as Xanthophyllomyces dendrorhous, Renilla reinformis and the human gene for pre-insulin codon optimised with our own codon-optimization tool for Y. lipolityca.
Laboratory safety measures
All work with genetically modified organisms (GMOs) were performed in class 1 GMO laboratories following the required procedures. This include amongst others wearing laboratory coats, gloves and goggles, disinfecting surfaces, tools etc. with ethanol and autoclaving all vaste, used tools and discarded cultures. We used laf-benches whenever possible to avoid the fire hazard of gas flames when performing sterile work. The Hamilton robot used as fermentation platform was decontaminated before and after each use with UV light.
In our work with hazardous substances we took extra safety measures and worked with utmost caution. We used laf-benches and powerful suction in the experiments performed with 2-Mercaptoethanol (BME). SYBR-Safe was always handled with gloves and caution. Containers with liquid nitrogen were only opened and cooling conducted in well ventilated rooms.
