Difference between revisions of "Team:ASIJ Tokyo/Parts"
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<h1>Parts </h1> | <h1>Parts </h1> | ||
<br> | <br> | ||
| − | <h3>Anderson Promoters</h3> | + | <h3><strong>Anderson Promoters</strong></h3> |
<li>Relative Strength: weak (0.33) BBa_J23110 | <li>Relative Strength: weak (0.33) BBa_J23110 | ||
<ul> | <ul> | ||
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</li> | </li> | ||
<br> | <br> | ||
| − | <h3>Tags</h3> | + | <h3><strong>Tags</strong></h3> |
<li>N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of e. Coli</li> | <li>N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of e. Coli</li> | ||
<li>C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression </li> | <li>C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression </li> | ||
<br> | <br> | ||
| − | <h3>Others</h3> | + | <h3><strong>Others</strong></h3> |
<li>PETase sequence: gene that coded for the PET hydrolase</li> | <li>PETase sequence: gene that coded for the PET hydrolase</li> | ||
<li>Plasmid backbone: the pSB1C3 backbone is recommended </li> | <li>Plasmid backbone: the pSB1C3 backbone is recommended </li> | ||
<li>High efficiency e. Coli cells: hosted the PETase constructs</li> | <li>High efficiency e. Coli cells: hosted the PETase constructs</li> | ||
<br> | <br> | ||
| − | <h3>Parts Submitted</h3> | + | <h3><strong>Parts Submitted</strong></h3> |
<li>BBa_K1885001</li> | <li>BBa_K1885001</li> | ||
<li>BBa_K1885002</li> | <li>BBa_K1885002</li> | ||
Revision as of 14:14, 19 October 2016
Parts
Anderson Promoters
- Selected as a relatively weak promoter against which to compare the expression of the PETase construct
- Selected as a relatively moderately strong promoter against which to compare the expression of the PETase construct
- Selected as a relatively strong promoter against which to compare the expression of the PETase construct
