Difference between revisions of "Team:ASIJ Tokyo/Parts"
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<h3><strong>Tags</strong></h3> | <h3><strong>Tags</strong></h3> | ||
| − | <li>N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of | + | <li>N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of <i>E. Coli</i></li> |
<li>C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression </li> | <li>C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression </li> | ||
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<li>PETase sequence: gene that coded for the PET hydrolase</li> | <li>PETase sequence: gene that coded for the PET hydrolase</li> | ||
<li>Plasmid backbone: the pSB1C3 backbone is recommended </li> | <li>Plasmid backbone: the pSB1C3 backbone is recommended </li> | ||
| − | <li>High efficiency | + | <li>High efficiency <i>E. Coli</i> cells: hosted the PETase constructs</li> |
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<h3><strong>Parts Submitted</strong></h3> | <h3><strong>Parts Submitted</strong></h3> | ||
Revision as of 14:16, 19 October 2016
Parts
Anderson Promoters
- Selected as a relatively weak promoter against which to compare the expression of the PETase construct
- Selected as a relatively moderately strong promoter against which to compare the expression of the PETase construct
- Selected as a relatively strong promoter against which to compare the expression of the PETase construct
