Chloroplast (Talk | contribs) |
Chloroplast (Talk | contribs) |
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<span>1 June, 2016</span> | <span>1 June, 2016</span> | ||
− | <a href="http://tympanus.net/Tutorials/TypographyEffects/">Milestone | + | <a href="http://tympanus.net/Tutorials/TypographyEffects/">Milestone 1 : Overall cloning design</a> |
</h3> | </h3> | ||
</div> | </div> | ||
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<div class="ss-row ss-large"> | <div class="ss-row ss-large"> | ||
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<h3> | <h3> | ||
<span>4 July, 2016</span> | <span>4 July, 2016</span> | ||
− | <a | + | <a>Milestone 3: : Construction of pLuxR-LuxI-HA </a> |
</h3> | </h3> | ||
</div> | </div> | ||
</div> | </div> | ||
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+ | <div class="ss-row ss-large"> | ||
+ | <div class="ss-left"> | ||
+ | <h3> | ||
+ | <span>11 July, 2016</span> | ||
+ | <a>Milestone 4: Screening of pLuxR-invasin constructs </a> | ||
+ | </h3> | ||
+ | </div> | ||
+ | <div class="ss-right ha-waypoint" data-animate-down="ha-header-rotate" data-animate-up="ha-header-rotateBack"> | ||
+ | <div class="log-content ss-circle-deco"> | ||
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+ | <p><img src="https://static.igem.org/mediawiki/2016/c/c6/T--NUS_Singapore--log3_16.png" style="width: 100%;"></p> | ||
+ | </div> | ||
+ | </div> | ||
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<div class="ss-row"> | <div class="ss-row"> | ||
<div class="ss-left"> | <div class="ss-left"> | ||
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</div> | </div> | ||
</div> | </div> | ||
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+ | <div class="ss-row ss-large"> | ||
+ | <div class="ss-left"> | ||
+ | <div class="log-content ss-circle-deco" style="margin-top: 20px;"> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2016/0/08/T--NUS_Singapore--log3_17.png" style="width: 100%;"></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="ss-right"> | ||
+ | <h3> | ||
+ | <span>1 Aug, 2016</span> | ||
+ | <a>Milestone 5: Screening of pLuxR-LLO construct</a> | ||
+ | </h3> | ||
+ | </div> | ||
+ | </div> | ||
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<div class="ss-row ss-large"> | <div class="ss-row ss-large"> | ||
<div class="ss-left"> | <div class="ss-left"> | ||
<h3> | <h3> | ||
− | <span> | + | <span>8 Aug, 2016</span> |
− | <a>Milestone | + | <a>Milestone 6: Construction of pLuxR-GFP plasmid</a> |
</h3> | </h3> | ||
</div> | </div> | ||
<div class="ss-right ha-waypoint" data-animate-down="ha-header-rotate" data-animate-up="ha-header-rotateBack"> | <div class="ss-right ha-waypoint" data-animate-down="ha-header-rotate" data-animate-up="ha-header-rotateBack"> | ||
<div class="log-content ss-circle-deco"> | <div class="log-content ss-circle-deco"> | ||
− | + | <p><img src="https://static.igem.org/mediawiki/2016/2/2b/T--NUS_Singapore--log3_18.png" style="width: 100%;"></p> | |
</div> | </div> | ||
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<div class="ss-row ss-large"> | <div class="ss-row ss-large"> | ||
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− | + | <div class="log-content ss-circle-deco" style="margin-top: 20px;"> | |
− | + | <p><img src="https://static.igem.org/mediawiki/2016/a/a3/T--NUS_Singapore--log3_19.png" style="width: 100%;"></p> | |
− | + | </div> | |
− | + | </div> | |
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<h3> | <h3> | ||
− | + | <span>1 Aug, 2016</span> | |
− | <a >Milestone | + | <a>Milestone 7: Testing the assembled pConst-LuxR-pLuxR-LuxI construct</a> |
</h3> | </h3> | ||
</div> | </div> | ||
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</div> | </div> | ||
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<div class="ss-row ss-large"> | <div class="ss-row ss-large"> | ||
− | + | <div class="ss-left"> | |
− | + | <h3> | |
− | + | <span>19 Sep, 2016</span> | |
− | + | <a>Milestone 8: Testing the assembled pConst-LuxR-pLuxR-LLO construct</a> | |
</h3> | </h3> | ||
− | </div> | + | </div> |
− | <div class="ss-right"> | + | <div class="ss-right ha-waypoint" data-animate-down="ha-header-rotate" data-animate-up="ha-header-rotateBack"> |
<div class="log-content ss-circle-deco"> | <div class="log-content ss-circle-deco"> | ||
− | + | <p><img src="https://static.igem.org/mediawiki/2016/b/bf/T--NUS_Singapore--log3_20.png" style="width: 100%;"></p> | |
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</div> | </div> | ||
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</div> | </div> | ||
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+ | <div class="ss-row ss-large"> | ||
<div class="ss-left"> | <div class="ss-left"> | ||
− | + | <div class="log-content ss-circle-deco" style="margin-top: 20px;"> | |
− | + | <p><img src="https://static.igem.org/mediawiki/2016/6/64/T--NUS_Singapore--log3_211.png" style="width: 100%;"></p> | |
− | + | </div> | |
− | + | </div> | |
− | + | <div class="ss-right"> | |
− | + | <h3> | |
− | <div class="log-content ss-circle-deco" | + | <span>10 Oct, 2016</span> |
− | + | <a>Milestone 9: Testing the assembled pConst-LuxR-pLuxR-GFP construct</a> | |
− | + | </h3> | |
− | + | </div> | |
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hasPerspective = false, | hasPerspective = false, | ||
− | perspective = hasPerspective | + | perspective = hasPerspective, |
// initialize function | // initialize function | ||
init = function() { | init = function() { |
Revision as of 15:03, 19 October 2016
<!DOCTYPE html>
Wet Lab Notebook: RIOT RESPONDER
Jun
2016
Overall Strategies
1 June, 2016 Milestone 1 : Overall cloning design
12 June, 2016 Milestone 2: Construction of pConst-LuxR-HA
Construct 1:
Fig 1. A. LuxR gene amplified from iGEM distribution 2016 with designed primers GEM002 and GEM005. The expected band size of LuxR with overhang is 795 bp. B.LuxR stitiched with terminater (BBa_B0015) with overlapping PCR. C. Constructed full-length RBS-pConstitutive-LuxR-HA-Terminator with expected size of 1034 bp.
July
2016
Construct 2:
Fig 2. A. LuxI gene is cloned from iGEM2016 distribution kit with primers GEM008 and GEM011. The observed band size of LuxI with overhangs is close to the expected band size of 629 bp. B.Terminator BBa_B0015 is amplified using GEM009 and 012 and stitiched with LuxI. C. Promoter-RBS fragment is synthesized and stitiched to LuxI-terminator by PCR reaction. The observed band size for full LuxI construct is close to the expected band size of 936 bp.