Difference between revisions of "Team:Aix-Marseille"

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The first step relies on the affinity of siderophores to bind solubilized Platinum atoms and, thus, favor the further solubilisation of more platinum compounds. We accomplish this by inserting a plasmid containing the four enzymes (Des A, Des B, Des C, Des D) necessary to synthesise our siderophore — Desferrioxamine B, into E. coli.
 
The first step relies on the affinity of siderophores to bind solubilized Platinum atoms and, thus, favor the further solubilisation of more platinum compounds. We accomplish this by inserting a plasmid containing the four enzymes (Des A, Des B, Des C, Des D) necessary to synthesise our siderophore — Desferrioxamine B, into E. coli.
  
At a second level, we plan to use the principle of biosorption. A modified fliC protein complex will be cloned into E. coli and enable the flagella of the bacterium to bind platinum atoms. This specificity will be possible thanks to a peptide that will be inserted into the sequence of the fliC.
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At a second level, we plan to use the principle of biosorption. A modified FliC protein complex will be cloned into E. coli and enable the flagella of the bacterium to bind platinum atoms. This specificity will be possible thanks to a peptide that will be inserted into the sequence of the FliC protein.
  
 
The benefit of using the biosorption is to obtain nanoparticles of platinum, a highly valuable form of the metal.
 
The benefit of using the biosorption is to obtain nanoparticles of platinum, a highly valuable form of the metal.
 
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Revision as of 14:57, 19 October 2016