Difference between revisions of "Team:NYMU-Taipei/Description"

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<h1>Achievement</h1><hr /><br />
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<b>Characterization</b>
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<p>
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This year we characterized and developed the part BBa_K1184000, phototoxic protein KillerRed, and a fungal selection marker gene <i>bar</i>.
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<b>KillerRed</b></p>
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<p>
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We characterized this part with thermal stability assay.</p>
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<p>KillerRed protein was extracted from transformed E.coli and a control group of empty vector was set to eliminate the influence of other proteins.
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This test aims to show the temperature influence on KillerRed protein by measuring its fluorescence after being treated for 2 hours at the following temperatures: 70, 70.5, 71.7, 73.5, 75.5, 78.3, 81, 83.6, 86, 88, 89.5, 92.2 (degree Celsius).
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[[img src="https://static.igem.org/mediawiki/parts/6/6b/Cha1.png" width="75%"]]
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Revision as of 19:43, 19 October 2016


Characterization

This year we characterized and developed the part BBa_K1184000, phototoxic protein KillerRed, and a fungal selection marker gene bar.

KillerRed

We characterized this part with thermal stability assay.

KillerRed protein was extracted from transformed E.coli and a control group of empty vector was set to eliminate the influence of other proteins. This test aims to show the temperature influence on KillerRed protein by measuring its fluorescence after being treated for 2 hours at the following temperatures: 70, 70.5, 71.7, 73.5, 75.5, 78.3, 81, 83.6, 86, 88, 89.5, 92.2 (degree Celsius).

[[img src="https://static.igem.org/mediawiki/parts/6/6b/Cha1.png" width="75%"]]