Difference between revisions of "Team:Aix-Marseille/Design"
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==Source of platinum== | ==Source of platinum== | ||
| − | Since a law from 1993, platinum is largely used in catalytic converter to avoid toxic gaz release <ref>J. de Aberasturi, et al., Minerals Engineering 24, 505 (2011)</ref>. . During the automobile functioning, the precious metal is rejected and deposed on the road under a ionic form <ref>P.S Hooda & al. 2007, https://www.ncbi.nlm.nih.gov/pubmed/17604084</ref><ref>Liliane Michel Legret & al. http://link.springer.com/article/10.1007/s11368-012-0491-3.</ref> Platinum leaching | + | Since a law from 1993, platinum is largely used in catalytic converter to avoid toxic gaz release <ref>J. de Aberasturi, et al., Minerals Engineering 24, 505 (2011)</ref>. . During the automobile functioning, the precious metal is rejected and deposed on the road under a ionic form <ref>P.S Hooda & al. 2007, https://www.ncbi.nlm.nih.gov/pubmed/17604084</ref><ref>Liliane Michel Legret & al. http://link.springer.com/article/10.1007/s11368-012-0491-3.</ref> Platinum leaching leads to an accumulation of this metal on the road side. By default, it has been prooved that plants are potential bioaccumulator of platinum, which is found concentrated on the roots, leaves.. Otherwise, the metal is also carried on the sewage sludge and is actually an issue regarding the recycling potential of these sludge. |
[[File:T--Aix-Marseille--platine.jpeg|500px|center|thumb|Platinum sources]] | [[File:T--Aix-Marseille--platine.jpeg|500px|center|thumb|Platinum sources]] | ||
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==Mobilisation by a siderophore== | ==Mobilisation by a siderophore== | ||
| − | In the previous article <ref> Improving recoveries of platinum and palladium from oxidized Platinum-group element ores of the Great Dyke, Zimbabwe, using the biogenic siderophore Desferrioxamine B, Dennis Kraemer & al. </ref>, authors made bioleaching with synthetised siderophores. By this way, they extracted approximately 80% of the total platinum found in the ore. But we were looking for a synthetic biological approach. | + | In the previous article <ref> Improving recoveries of platinum and palladium from oxidized Platinum-group element ores of the Great Dyke, Zimbabwe, using the biogenic siderophore Desferrioxamine B, Dennis Kraemer & al. </ref>, authors made bioleaching with chemically synthetised siderophores. By this way, they extracted approximately 80% of the total platinum found in the ore. But we were looking for a synthetic biological approach. A biological approach can lead to an easier and cheaper way to extract minerals. |
===Pathway of the desferrioxamine B biosynthesis=== | ===Pathway of the desferrioxamine B biosynthesis=== | ||
[[File:T--Aix-Marseille--desABCD.jpeg|600px|center|thumb|Desferrioxamine B pathway]] | [[File:T--Aix-Marseille--desABCD.jpeg|600px|center|thumb|Desferrioxamine B pathway]] | ||
| − | Previous work showed that siderophores which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Specifically, many articles showed a high affinity of Desferrioxamine B ( produce by ''Streptomyces coelicolor'') for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. <i>E. coli</i> was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive <ref> Wandersman & Delepaire https://www.ncbi.nlm.nih.gov/pubmed/15487950 </ref> , considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate transcription using the | + | Previous work showed that siderophores which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Specifically, many articles showed a high affinity of Desferrioxamine B ( produce by ''Streptomyces coelicolor'') for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. <i>E. coli</i> was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive <ref> Wandersman & Delepaire https://www.ncbi.nlm.nih.gov/pubmed/15487950 </ref> , considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate transcription using the arabinose inductible promoter, pBAD/araC |
| − | It has to be noted that the siderophore here is specific for platinum but the same cloning could be performed with siderophore specific for others metals, thus the potential of our process to be applied in the recovery of others metals. | + | It has to be noted that the siderophore here is specific for platinum but the same cloning could be performed with siderophore specific for others metals, thus the potential of our process to be applied in the recovery of others metals thanks to synthetic biology and its modularity. |
===Our strategy=== | ===Our strategy=== | ||
