Difference between revisions of "Team:Edinburgh OG/Notebook"

Line 90: Line 90:
 
                                 <div class="timeline-body">
 
                                 <div class="timeline-body">
 
                                     <p class="text-muted">
 
                                     <p class="text-muted">
−
                                       <br>Our first day in the lab, where we found our beloved starter kit. The following 2 weeks were focused on literature research, ordering of reagents and kits, lab preparation and finding sponsors for our project. The team also received training on Benchling and MoClo, which we expect we are going to use very frequently in the next few months.<br>
+
                                       <br>Our first day in the lab, where we found our beloved starter kit. The following 2 weeks were focused on literature research, ordering of reagents and kits, lab preparation and finding sponsors for our project. The team also received an introduction to MoClo and training in Benchling, which we relied on heavily over the next few months.<br>
 
                                       <br>The rhodococcus team started growing Rhodoccocus jostii on plates. The synechocystis team received the pPMQAK1 plasmid to be used for cloning in E. coli before transformation into Synechocystis. <br>
 
                                       <br>The rhodococcus team started growing Rhodoccocus jostii on plates. The synechocystis team received the pPMQAK1 plasmid to be used for cloning in E. coli before transformation into Synechocystis. <br>
 
                                       <br>Team MoClo: Received CIDAR MoClo plasmids from Laura Tuck <br>
 
                                       <br>Team MoClo: Received CIDAR MoClo plasmids from Laura Tuck <br>

Revision as of 19:12, 19 October 2016

-->

Notebook

  • January 2016

    Our Humble Beginnings

    Our group members each sign up to be the first Edinburgh Overgraduate iGEM team!

  • February 1 - 14th 2016

    Brainstorming Session

    The team meet together for the first time and brainstorm initial ideas for the project.

  • February 15th - 17th 2016

    Innovative Learning Week

    During Innovative Learning Week the team head to the Firbush Outdoor Centre in the Scottish Highlands for some canoeing, team building and a quick dip in an ice cold loch! Oh and to pitch our ideas for project too, of course.

  • April 2016

    Exams Done

    With our exams and assignments from semester 2 complete we set about outlining our project and identifying a dissertation project for each member.

  • May 9th-20th 2016

    Lab Started


    Our first day in the lab, where we found our beloved starter kit. The following 2 weeks were focused on literature research, ordering of reagents and kits, lab preparation and finding sponsors for our project. The team also received an introduction to MoClo and training in Benchling, which we relied on heavily over the next few months.

    The rhodococcus team started growing Rhodoccocus jostii on plates. The synechocystis team received the pPMQAK1 plasmid to be used for cloning in E. coli before transformation into Synechocystis.

    Team MoClo: Received CIDAR MoClo plasmids from Laura Tuck

    Penicillium team?

  • May 23rd-June 3rd 2016

    Dissertation is coming


    After much deliberation, we finally decided to use the Bba_ P10500 Phytobrick Universal Acceptor Vector as our destination vector for all our (level 0) MoClo compatible DNA parts. The order for the synthesis of DNA gblocks for our parts was placed through IDT.

    Team MoClo: Finished Primer design for mutagenesis & start cloning plasmids for template

  • June 6th-June 17th 2016

    Dissertation is coming


    Our first MoClo assembly was finally performed using DNA parts found in the CIDAR MoClo kit. However, very few colonies were obtained and therefore our MoClo reaction mixture and conditions were adapted.

    Team MoClo: Mutagenesis & screening of illegal removal sites for Rhodococcus vectors. Mut2 and Mut3 showed a good result.

  • June 20th-July 1st 2016

    Dissertation is coming

    Lorem ipsum dolor sit amet, consectetur adipisicing elit. Sunt ut voluptatum eius sapiente, totam reiciendis temporibus qui quibusdam, recusandae sit vero unde, sed, incidunt et ea quo dolore laudantium consectetur!

  • July 4th-July 15th 2016

    Our Humble Beginnings

    Lorem ipsum dolor sit amet, consectetur adipisicing elit. Sunt ut voluptatum eius sapiente, totam reiciendis temporibus qui quibusdam, recusandae sit vero unde, sed, incidunt et ea quo dolore laudantium consectetur!

  • July 18th-July 30th 2016

    Lab Started!

    Lorem ipsum dolor sit amet, consectetur adipisicing elit. Sunt ut voluptatum eius sapiente, totam reiciendis temporibus qui quibusdam, recusandae sit vero unde, sed, incidunt et ea quo dolore laudantium consectetur!

  • August 1st-August 18th 2016

    Full Throttle

    Lorem ipsum dolor sit amet, consectetur adipisicing elit. Sunt ut voluptatum eius sapiente, totam reiciendis temporibus qui quibusdam, recusandae sit vero unde, sed, incidunt et ea quo dolore laudantium consectetur!

  • August 15th-August 27th 2016

    Dissertation is coming

    Lorem ipsum dolor sit amet, consectetur adipisicing elit. Sunt ut voluptatum eius sapiente, totam reiciendis temporibus qui quibusdam, recusandae sit vero unde, sed, incidunt et ea quo dolore laudantium consectetur!

  • Full Throttle

    Lorem ipsum dolor sit amet, consectetur adipisicing elit. Sunt ut voluptatum eius sapiente, totam reiciendis temporibus qui quibusdam, recusandae sit vero unde, sed, incidunt et ea quo dolore laudantium consectetur!

  • Dissertation is coming

    Lorem ipsum dolor sit amet, consectetur adipisicing elit. Sunt ut voluptatum eius sapiente, totam reiciendis temporibus qui quibusdam, recusandae sit vero unde, sed, incidunt et ea quo dolore laudantium consectetur!

  • Be Part
    Of Our
    Story!