Difference between revisions of "Team:Aix-Marseille/Design"
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==Source of platinum== | ==Source of platinum== | ||
| − | Since a law from 1993, platinum is largely used in catalytic converter to avoid toxic gaz release <ref>J. de Aberasturi, et al., Minerals Engineering 24, 505 (2011)</ref>. . During the automobile functioning, the precious metal is rejected and deposed on the road under a ionic form <ref>P.S Hooda & al. 2007, https://www.ncbi.nlm.nih.gov/pubmed/17604084</ref><ref>Liliane Michel Legret & al. http://link.springer.com/article/10.1007/s11368-012-0491-3.</ref> Platinum leaching leads to an accumulation of this metal on the road side. By default, it has been prooved that plants are potential bioaccumulator of platinum, which is found | + | Since a law from 1993, platinum is largely used in catalytic converter to avoid toxic gaz release <ref>J. de Aberasturi, et al., Minerals Engineering 24, 505 (2011)</ref>. . During the automobile functioning, the precious metal is rejected and deposed on the road under a ionic form <ref>P.S Hooda & al. 2007, https://www.ncbi.nlm.nih.gov/pubmed/17604084</ref><ref>Liliane Michel Legret & al. http://link.springer.com/article/10.1007/s11368-012-0491-3.</ref> Platinum leaching leads to an accumulation of this metal on the road side. By default, it has been prooved that plants are potential bioaccumulator of platinum, which is found accumulated on the roots, leaves or into them. Otherwise, the metal is also carried on the sewage sludge and is actually an issue regarding the recycling potential of these sludge. |
[[File:T--Aix-Marseille--platine.jpeg|500px|center|thumb|Platinum sources]] | [[File:T--Aix-Marseille--platine.jpeg|500px|center|thumb|Platinum sources]] | ||
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This step aims to : | This step aims to : | ||
* adsorb ions on bacterial flagella protein | * adsorb ions on bacterial flagella protein | ||
| − | * | + | * reduces ions into oxidized nanoparticles by the ambient reducer power |
All together, these findings incite us to use these natural properties to build a biobrick which is a high affinity binder of platinum based on <i>E. coli</i> and <i>Desulfovibrio vulgaris</i> flagellum and synthetic peptides. To this end, we analyze the flagella sequences and structural properties of the external part of the flagella. Then, on the part of the flagellin facing the external medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorption specificity and yield. In this way, peptide would be facing the external medium and being able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promoter enabling a high flagellin production. | All together, these findings incite us to use these natural properties to build a biobrick which is a high affinity binder of platinum based on <i>E. coli</i> and <i>Desulfovibrio vulgaris</i> flagellum and synthetic peptides. To this end, we analyze the flagella sequences and structural properties of the external part of the flagella. Then, on the part of the flagellin facing the external medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorption specificity and yield. In this way, peptide would be facing the external medium and being able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promoter enabling a high flagellin production. | ||
[[File:T--Aix-Marseille--flagel_captation.jpeg|500px|center|thumb|Adsorption of the platinum on the flagellin]] | [[File:T--Aix-Marseille--flagel_captation.jpeg|500px|center|thumb|Adsorption of the platinum on the flagellin]] | ||
