Difference between revisions of "Team:NYMU-Taipei/Description"

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<img src="https://static.igem.org/mediawiki/parts/6/6b/Cha1.png" width="75%"/>
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<img src="https://static.igem.org/mediawiki/parts/6/6b/Cha1.png" width="65%">
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<p>Figure1: Result of heat denaturation experiment
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It is obvious that KR contribute to most of the fluorescence.
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<img src="https://static.igem.org/mediawiki/parts/7/78/Cha2.jpeg" width="60%">
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<p>Figure2: Curve fitting of KillerRed fluorescence
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<p>A sigmoidal curve was fitted to find the particular temperature K at which half of the KR protein is denatured studied by measuring the fluorescence.
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<img src="https://static.igem.org/mediawiki/parts/c/c3/Cha_formula.png" width="75%">
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A sigmoidal curve was fitted to find the particular temperature K at which half of the KR protein is denatured studied by measuring the fluorescence.
  
  

Revision as of 19:56, 19 October 2016

Characterization

This year we characterized and developed the part BBa_K1184000, phototoxic protein KillerRed, and a fungal selection marker gene bar BBa_K1021003.

KillerRed

We characterized this part with thermal stability assay.

KillerRed protein was extracted from transformed E.coli and a control group of empty vector was set to eliminate the influence of other proteins. This test aims to show the temperature influence on KillerRed protein by measuring its fluorescence after being treated for 2 hours at the following temperatures: 70, 70.5, 71.7, 73.5, 75.5, 78.3, 81, 83.6, 86, 88, 89.5, 92.2 (degree Celsius).

Figure1: Result of heat denaturation experiment

It is obvious that KR contribute to most of the fluorescence.

Figure2: Curve fitting of KillerRed fluorescence

A sigmoidal curve was fitted to find the particular temperature K at which half of the KR protein is denatured studied by measuring the fluorescence.

A sigmoidal curve was fitted to find the particular temperature K at which half of the KR protein is denatured studied by measuring the fluorescence.