Difference between revisions of "Team:Aix-Marseille/Results"

(Futur plan for our project)
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- <i>E. coli</i>  TG1 strain with pSB1C3 containing the RFP coding sequence (negative control)
 
- <i>E. coli</i>  TG1 strain with pSB1C3 containing the RFP coding sequence (negative control)
  
- <i>E. coli</i>  TG1 strain with des operon ([http://parts.igem.org/Part:BBa_K1951011 BBa_K1951011]) before and after induction. (You can observe the production of the 4 proteins on the figure on the right; left : before induction, right : after induction)
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- <i>E. coli</i>  TG1 strain with <i>des</i> operon ([http://parts.igem.org/Part:BBa_K1951011 BBa_K1951011]) before and after induction. (You can observe the production of the 4 proteins on the figure on the right; left : before induction, right : after induction)
  
 
Results showed the production of the 4 proteins DesA, DesB, DesC and DesD, all involved in the desferrioxamine B biosynthesis pathway.
 
Results showed the production of the 4 proteins DesA, DesB, DesC and DesD, all involved in the desferrioxamine B biosynthesis pathway.
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=== Proof of fonctionnality===
 
=== Proof of fonctionnality===
 
We investigated if DesA (Lysine decarboxylase) was fonctionnal by measurement of cadaverine using HPLC with C18 column and we proved that our biobrick allows the production of DesA which enables to produce the cadaverine and makes it fonctionnal.   
 
We investigated if DesA (Lysine decarboxylase) was fonctionnal by measurement of cadaverine using HPLC with C18 column and we proved that our biobrick allows the production of DesA which enables to produce the cadaverine and makes it fonctionnal.   
[[File:T--Aix-Marseille--result5.jpeg|500px|tight|thumb| Investigation of the cadaverine production by the lysine decarboxylase DesA. The cadaverin production has been detected by HPLC using C18 column after induction of the strain. Different backgrounds were analysed : wild type <i>Escherichia coli</i> TG1 strain (yellow column), cadA mutant from Keio bank (blue column), complemented <i> cadA </i> mutant from Keio bank with [http://parts.igem.org/Part:BBa_K1951004 Bba_K1951004](orange column), complemented <i>cadA</i> mutant from Keio bank with [http://parts.igem.org/Part:BBa_K1951004Bba_K1951011(grey column).]]
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[[File:T--Aix-Marseille--result5.jpeg|500px|tight|thumb| Investigation of the cadaverine production by the lysine decarboxylase DesA. The cadaverin production has been detected by HPLC using C18 column after induction of the <i>des</i> genes. Different backgrounds were analysed : wild type <i>Escherichia coli</i> TG1 strain (yellow column), <i>cadA</i> mutant from Keio bank (blue column), complemented <i> cadA </i> mutant from Keio bank with p<i>cadA</i> ([http://parts.igem.org/Part:BBa_K1951004 Bba_K1951004])(orange column), complemented <i>cadA</i> mutant from Keio bank with <i>des</i> operon ([http://parts.igem.org/Part:BBa_K1951011 Bba_K1951011])(grey column).]]
  
 
Results showed cadaverine detection in the wild type meaning the original strain well produces the cadaverine.
 
Results showed cadaverine detection in the wild type meaning the original strain well produces the cadaverine.

Revision as of 21:08, 19 October 2016