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<p1>Sequences for the SUSr repressor gene and SUS operator and promoter, were taken from the NCBI Genbank database. The Nma111p gene will be under control of a weak constitutive promoter taken from the iGEM Parts registry, the minimal cyc promoter (Part: BBa_I766557), for example. </p1> | <p1>Sequences for the SUSr repressor gene and SUS operator and promoter, were taken from the NCBI Genbank database. The Nma111p gene will be under control of a weak constitutive promoter taken from the iGEM Parts registry, the minimal cyc promoter (Part: BBa_I766557), for example. </p1> | ||
− | <p1>With guidance from Dr.Looger, we were able to begin designing the primers and DNA templates for homologous recombination, based upon the following | + | <p1>With guidance from Dr.Looger, we were able to begin designing the primers and DNA templates for homologous recombination, based upon the following <a href="http://research.stowers.org/jaspersenlab/docs/markerswitching.pdf">protocol for homologous recombination</a>: </p1> |
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<p1>However, soon after starting these designs in early August, with plans to synthesize them and test our kill switch design, we found ourselves without a microbiology lab to work in. Our schools did not have the proper resources to handle complex techniques such as homologous recombination, nor the funding for supplies. We were unable to get approved to work in a laboratory at local universities and research institutions either. While we were not able to carry out our design for the kill switch, we hope that our design may be useful for other iGEM teams looking into kill switches for yeast, an area for which there is not much research. | <p1>However, soon after starting these designs in early August, with plans to synthesize them and test our kill switch design, we found ourselves without a microbiology lab to work in. Our schools did not have the proper resources to handle complex techniques such as homologous recombination, nor the funding for supplies. We were unable to get approved to work in a laboratory at local universities and research institutions either. While we were not able to carry out our design for the kill switch, we hope that our design may be useful for other iGEM teams looking into kill switches for yeast, an area for which there is not much research. | ||
</p1> | </p1> | ||
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<h4>References</h4> | <h4>References</h4> | ||
<p1>1.Madeo, F., Herker, E., Maldener, C., Wissing, S., Lachelt, S., Herlan, M., Fehr, M., Lauber, K., Sigrist, S. J., Wesselborg, S., et al. (2002) A caspase-related protease regulates apoptosis in yeast. Mol. Cell 9, 1– 120.</p1> | <p1>1.Madeo, F., Herker, E., Maldener, C., Wissing, S., Lachelt, S., Herlan, M., Fehr, M., Lauber, K., Sigrist, S. J., Wesselborg, S., et al. (2002) A caspase-related protease regulates apoptosis in yeast. Mol. Cell 9, 1– 120.</p1> |
Latest revision as of 21:42, 19 October 2016
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Project Design
Pre-Design considerations
1.Moe-Behrens, G. H. G., Davis, R., & Haynes, K. A. (2013). Preparing synthetic biology for the world. Frontiers in Microbiology, 4, 5. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3554958/
2.Dana GV, Kuiken T, Rejeski D, Snow AA Synthetic biology: Four steps to avoid a synthetic-biology disaster.Nature. 2012 Feb 29; 483(7387):29.