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<specialh3>Colour proof</specialh3><br><br> | <specialh3>Colour proof</specialh3><br><br> | ||
<p> | <p> | ||
− | In order to demonstrate one of the many possible applications of our ratio control circuit, we decided to use chromoproteins as visual proof of concept. We were able to create a multitude of colours by mixing different ratios of <i>E. coli</i> cultures expressing the chromoproteins. We hope that this provided a glimpse of the power of co-culture in the production of composite biomaterials. <br><br> | + | In order to demonstrate one of the many possible applications of our ratio control circuit, we decided to use chromoproteins as visual proof of concept. We were able to create a multitude of colours by mixing different ratios of <i>E. coli</i> cultures expressing the chromoproteins. We hope that this provided a glimpse of the power of co-culture in the production of composite biomaterials. <br><br></p> |
<specialh3>Key Achievements</specialh3><ul style="font-size:18px;"> | <specialh3>Key Achievements</specialh3><ul style="font-size:18px;"> | ||
<li>Built constructs for expressing 7 different chromoproteins </li> | <li>Built constructs for expressing 7 different chromoproteins </li> | ||
<li>Produced a construct linking GP2 expression to chromoprotein expression </li> | <li>Produced a construct linking GP2 expression to chromoprotein expression </li> | ||
<li>Showed that Gp2 can be used to control the growth of cells and consequently the production of proteins</li></ul> | <li>Showed that Gp2 can be used to control the growth of cells and consequently the production of proteins</li></ul> | ||
− | + | <p> | |
<specialh3>Overview</specialh3><br><br> | <specialh3>Overview</specialh3><br><br> | ||
Chromoproteins are brightly coloured when expressed, and the colour is clearly visible to the naked eye, unlike fluorescent proteins. We want to use these proteins to produce coloured cells to demonstrate how co-cultures can be used to create varying shades of colour, providing a visual reference for the ratio of two cells. Chromoproteins are of great interest to the synthetic biology community for used as reporters and also as a new form of non-toxic biological pigments. Colours provide a simple way of demonstrating how different ratio and composition of materials can generate an array of products with different properties. <br><br> | Chromoproteins are brightly coloured when expressed, and the colour is clearly visible to the naked eye, unlike fluorescent proteins. We want to use these proteins to produce coloured cells to demonstrate how co-cultures can be used to create varying shades of colour, providing a visual reference for the ratio of two cells. Chromoproteins are of great interest to the synthetic biology community for used as reporters and also as a new form of non-toxic biological pigments. Colours provide a simple way of demonstrating how different ratio and composition of materials can generate an array of products with different properties. <br><br> |
Revision as of 22:16, 19 October 2016