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− | In order to characterize our enzyme, it was necessary to determine if the enzyme had actually worked. This was done by graphing the data from the BadA lysate and the Empty Vector lysate (see graph below). In the graph below, it is possible to see that the absorption within the BadA lysate went down farther and faster than the Empty Vector lysate. This indicates that the enzyme is active and is working. | + | <h2>Does BadA Work?</h2> |
+ | <p> In order to characterize our enzyme, it was necessary to determine if the enzyme had actually worked. This was done by graphing the data from the BadA lysate and the Empty Vector lysate (see graph below). In the graph below, it is possible to see that the absorption within the BadA lysate went down farther and faster than the Empty Vector lysate. This indicates that the enzyme is active and is working. </p> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/3/3a/T--Duke--Comp2EV.png"> | ||
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<img src="https://static.igem.org/mediawiki/2016/6/65/T--Duke--BadAControls.png"> | <img src="https://static.igem.org/mediawiki/2016/6/65/T--Duke--BadAControls.png"> | ||
<img src="https://static.igem.org/mediawiki/2016/b/bf/T--Duke--BadAGraph1.png"> | <img src="https://static.igem.org/mediawiki/2016/b/bf/T--Duke--BadAGraph1.png"> | ||
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<img src="https://static.igem.org/mediawiki/2016/f/f4/T--Duke--SlopeExample.png"> | <img src="https://static.igem.org/mediawiki/2016/f/f4/T--Duke--SlopeExample.png"> |
Revision as of 01:38, 20 October 2016
Does BadA Work?
In order to characterize our enzyme, it was necessary to determine if the enzyme had actually worked. This was done by graphing the data from the BadA lysate and the Empty Vector lysate (see graph below). In the graph below, it is possible to see that the absorption within the BadA lysate went down farther and faster than the Empty Vector lysate. This indicates that the enzyme is active and is working.