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Penicillium roqueforti, while GRAS, is known to produce mycotoxins that are potentially lethal to humans when it certain growth conditions. Potential toxicity is not well understood however the organism has been widely used in food production for over 1,000 years. To mitigate any risk in genetic modification, whenever possible work was carried out in a laminar flow hood and in a separate laboratory from work conducted by other groups.Further, media in which lowered mycotoxin production has been observed was used for cell culture. | Penicillium roqueforti, while GRAS, is known to produce mycotoxins that are potentially lethal to humans when it certain growth conditions. Potential toxicity is not well understood however the organism has been widely used in food production for over 1,000 years. To mitigate any risk in genetic modification, whenever possible work was carried out in a laminar flow hood and in a separate laboratory from work conducted by other groups.Further, media in which lowered mycotoxin production has been observed was used for cell culture. | ||
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− | Typical antibiotics are ineffective in selection screening with Penicillium roqueforti however success with phleomycin screens is widely reported. This highly toxic group of glycopeptide antibiotics poses risk to human health however no safer alternative could be found. | + | Typical antibiotics are ineffective in selection screening with Penicillium roqueforti however success with phleomycin screens is widely reported. This highly toxic group of glycopeptide antibiotics poses risk to human health however no safer alternative could be found. To minimise any risk posed by the requirement to use this toxic substance, phleomycin was used only as low concentration liquid solutions and only handled within a laminar flow hood. All materials that made contact with phleomycin solutions were repeatedly washed and then disposed of in a cytotoxic bin following appropriate procedures. |
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Latest revision as of 02:01, 20 October 2016
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