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| − | <p>Solid tumor cells compete with normal tissues in all kinds of nutrition, and therefore can be considered as harmful mutants in the ecosystem in vivo. We propose a novel approach to eliminate tumor cells by utilizing microorganisms that share high ecological niche with tumor cells. Bifidobacterium has been proved to be non-immunogenic to human body, and has the ability to settle in hypoxic regions, such as solid tumors, preferentially. Based on what described above, we planned to construct a recombinant plasmid, which consist of DNA replication origins and related genes from pMB-1, a bifidobacterial plasmid, and pUC18, a plasmid commonly used in E.coil. Furthermore, we have cloned a HU, a Bifidobacterium promoter, and apoptin gene into this recombinant plasmid. As a result, the plasmid can be amplified both in E.coil. and Bifidobacterium to achieve the ability to kill tumor cells. In addition, we have tried to inject Bifidobacterium into tumor-bearing mice, and simulate the distribution and association of Bifidobacterium with tumor cells using ecological competition models. After the tumor-killing ability of this recombinant plasmid is proven, we will focus on enhancing the biosafety of our system through modifying the HU promoter activity to regulate the apoptin expression and applying a conditional lethal mechanism to restrict the proliferation of Bifidobacteriun.</p>
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| − | <p>The project was discussed for a long time. At the beginning, choosing a proper gene to kill the tumor cells was really difficult. We finally chose apoptin. It is a small molecular mass protein that only induces the apoptosis of tumor cells and does no affect the survival of normal cells. Moreover, after reading many research articles, we chose Bifidobacterium Long to carry the plasmid, which could target on the anaerobic region of solid tumor.
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