Difference between revisions of "Team:Alverno CA/InterLab"
| Line 17: | Line 17: | ||
<p><h3>Project Description:</h3></p> | <p><h3>Project Description:</h3></p> | ||
| − | <p>We tested 3 different fluorescent protein expression plasmids, J23101, J23106, and J23117. We transformed the parts with iGEM's transformation protocol. Our bacteria was cultured on a LB agar plate and cultured in LB broth. To measure the differences in fluorescence caused by each plasmid, we used a plate reader with | + | <p>We tested 3 different fluorescent protein expression plasmids, J23101, J23106, and J23117. We transformed the parts with iGEM's transformation protocol. Our bacteria was cultured on a LB agar plate and cultured in LB broth. To measure the differences in fluorescence caused by each plasmid, we used a plate reader with an OD600 reference point and FITC standard curve.<p> |
<p><h3>Protocol:</h3> </p> | <p><h3>Protocol:</h3> </p> | ||
| − | <p> | + | <p><a href="https://static.igem.org/mediawiki/2016/c/c5/InterLab_iGEM2016_Plate_Reader_Protocol_Updated_July.pdf">Plate Reader Protocol</a></p> |
| + | <p><a href="https://static.igem.org/mediawiki/parts/6/67/IGEM_Registry_-_Transformation_Protocol.pdf">Transformation Protocol</a></p> | ||
<p><h3>Results:</h3></p> | <p><h3>Results:</h3></p> | ||
Revision as of 19:21, 12 August 2016
Alverno iGEM Interlab Study
Quantifying different sources of variation in gene expression using
fluorescent transformed E. coli bacteria
Summary:
Leverage agile frameworks to provide a robust synopsis for high level overviews. Iterative approaches to corporate strategy foster collaborative thinking to further the overall value proposition. Organically grow the holistic world view of disruptive innovation via workplace diversity and empowerment.
Project Description:
We tested 3 different fluorescent protein expression plasmids, J23101, J23106, and J23117. We transformed the parts with iGEM's transformation protocol. Our bacteria was cultured on a LB agar plate and cultured in LB broth. To measure the differences in fluorescence caused by each plasmid, we used a plate reader with an OD600 reference point and FITC standard curve.
