Revision as of 06:40, 25 August 2016

Cellfiefuge Project description

Overview

Design

Protein production module

Auto aggregation module

×

Overview

Since the advent of rDNA technology in the late 1970s, the use of recombinant protein has been on the rise, starting from insulin and hormones all the way through to monoclonal antibodies and one hundred and fifty-one FDA approved protein based recombinant pharmaceuticals by the year 2009[1]. Despite being in high demand, recombinant proteins have heavy price tags, thanks to the significant manufacturing costs. One of the important steps in the manufacturing process is Centrifugation but on an industrial scale, it is an economic nightmare. [2] Further, existing industrial setups use complex sensors to monitor cell density to determine the point at which to begin separation of the cells from the cell culture. One can immediately see the humongous relief that would be associated with finding a way to deal with the above problems. Hence, we came up with the idea of our project, the Cellfifuge.

×

Our Bioreactor Design

We realized that manufacturing units use expensive and energy expensive machinery [3]. Hence, we engineered our bacteria in such a way that it could do most of the work done by these monstrous machines so that we no longer have to buy them and pay their bills. To increase the yield of the product, we came up with the idea of separating cell growth and target protein production so that when the cells have grown up to a high enough cell density, they shuttle all their energy towards the required protein production.[4] Based on the above ideas, we came up with the design of our bioreactor. (Figure 1) We realized that we would not be able to work on the whole idea this summer so, we focussed on two modules of our design: the Protein production module and the auto-aggregation module leaving the arresting of cell growth for future iGEM teams as a seed for their ideas.

×

Protein Production Module

Induction synthesis of protein of interest is another important step. Microorganisms in bioreactors are induced at the OD, at which the protein production is maximum. [5] Because of this, factories install sophisticated machinery to keep track of the OD value of the culture so as to know when to induce the production. We wanted our bacteria to know when to start protein production and start it by itself. Hence, we used the lux quorum sensing system to sense this cell density. [6] We have expressed the gene for our protein of interest under the luxI gene so that protein of interest is produced in significant quantities only when the quorum is achieved.(Figure 2) We also wish to tune the quorum sensing system by tweaking with the strength of the promoters involved in quorum sensing.

×

Protein Production Module

Induction synthesis of protein of interest is another important step. Microorganisms in bioreactors are induced at the OD, at which the protein production is maximum. [6] Because of this, factories install sophisticated machinery to keep track of the OD value of the culture so as to know when to induce the production. We wanted our bacteria to know when to start protein production and start it by itself. Hence, we used the lux quorum sensing system to sense this cell density. [7] We have expressed the gene for our protein of interest under the luxI gene so that protein of interest is produced in significant quantities only when the quorum is achieved.(Figure 2) We also wish to tune the quorum sensing system by tweaking with the strength of the promoters involved in quorum sensing.

References

[1]Ferrer-Miralles N, Domingo-Espin J, Corchero JL, Vazquez E, Villaverde A. Microbial factories for recombinant pharmaceuticals, Microbial Cell Factories, 2009:8:17.

[2]Medipally S.R., Yusoff F. Md., Banerjee S., Shariff M. Microalgae as sustainable renewable energy Feedstock for Biofuel Production, BioMed Research International, 2015.

[3]Felo M., Christensen B., Higgins J., Process cost and facility considerations in the selection of primary cell culture clarification technology, AIChE, 2013.

[4]Motoo Suzuki, Rohini Roy, Haiyan Zheng, Nancy Woychik, Masayori Inouye, Bacterial Bioreactors for High Yield Production of Recombinant Protein. The Journal of Biological Chemistry, 2006.

[5]Rosano G.L., Ceccarelli E. A., Recombinant protein expression in Escherichia coli: advances and challenges, Frontiers in Microbiology, 2014.

[6]Miller M.B., Bassler B.L., Quorum Sensing in Bacteria, Annual Review of Microbiology, 2001.

[7]Marjan W. van der Woude, Ian R. Henderson, Regulation and Function of Ag43 (Flu), Annu. Rev. Microbiol., 2008.

@@ Line 1: / Line 1: @@
 {{IISc Bangalore/hide}}
-{{Template:IISc Bngalore/boot css}}
+{{Template:IISc Bangalore/projectContent.css}}
+{{Template:IISc Bangalore/projectSidebar.css}}
+{{Template:IISc Bangalore/projectSidebar.js}}
+{{Template:IISc Bangalore/projectBoxes.css}}
 <html>
    <head>
@@ Line 6: / Line 9: @@
    <meta name="viewport" content="width=device-width, initial-scale=1">
    <link rel="stylesheet" href="http://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
+  <link rel="stylesheet" type="text/css" href="bootstrap-3.3.7-dist/css/bootstrap.min.css">
+  <link rel="stylesheet" type="text/css" href="bootstrap-3.3.7-dist/css/bootstrap.min.css.map">
+  <link rel="stylesheet" type="text/css" href="bootstrap-3.3.7-dist/css/bootstrap-theme.min.css.map">
+  <link rel="stylesheet" type="text/css" href="bootstrap-3.3.7-dist/css/bootstrap-theme.min.css">
+  <link rel="stylesheet" type="text/css" href="content5.css">
+  <link rel="stylesheet" type="text/css" href="sidebar5.css">
+  <link rel="stylesheet" type="text/css" href="boxes6.css">
+  <style type="text/css">
+  @font-face {
+      font-family: "Montserrat";
+      src: url(fonts/Monteserrat/Montserrat-Regular.ttf) format("truetype");
+  }
+  /*p.customfont {
+      font-family: "My Custom Font", Verdana, Tahoma;
+  }*/
+  </style>
+    <!-- <link rel="stylesheet" type="text/css" href="navbar5.css"> -->
+  <!-- <script src="https://use.fontawesome.com/29ffc57809.js"></script> -->
    <link href="http://fonts.googleapis.com/css?family=Montserrat" rel="stylesheet" type="text/css">
    <link href="http://fonts.googleapis.com/css?family=Lato" rel="stylesheet" type="text/css">
+  <link rel="stylesheet" type="text/css" href="bootstrap-3.3.7-dist/fonts/glyphicons-halflings-regular.woff">
    <script src="https://ajax.googleapis.com/ajax/libs/jquery/1.12.4/jquery.min.js"></script>
    <script src="http://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
+  <script src="sidebar5.js"></script>
    </head>
-   <body>
+   <body data-spy="scroll" data-target="#sidebar">
+    <!-- <span >
+<svg class="panel" onclick="openBar()" enable-background="new 0 0 128 128" height="40px" id="Layer_1" version="1.1" viewBox="0 0 128 128" width="40px" xml:space="preserve" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g><g><path d="M64,0C28.656,0,0,28.656,0,64s28.656,64,64,64s64-28.656,64-64S99.344,0,64,0z M64,120    C33.125,120,8,94.875,8,64S33.125,8,64,8s56,25.125,56,56S94.875,120,64,120z" fill="#B0BEC5"/></g></g><g><g><path d="M96,56H72V32c0-4.414-3.586-8-8-8s-8,3.586-8,8v24H32c-4.414,0-8,3.586-8,8s3.586,8,8,8h24v24    c0,4.414,3.586,8,8,8s8-3.586,8-8V72h24c4.414,0,8-3.586,8-8S100.414,56,96,56z" fill="#8BC34A"/></g></g></svg>
+    </span> -->
        <nav class="navbar navbar-fixed-top text-center navbar-inverse">
          <div class="container-fluid">
@@ Line 24: / Line 53: @@
          <span class="icon-bar"></span>
        </button>
-           <a class="navbar-brand" href="#">On this page <span class="glyphicon glyphicon-th
+           <a class="navbar-brand panel" onclick="openBar()" href="#" >On this page <span class="glyphicon glyphicon-th
 "></span></a> </div>
          <div class="collapse navbar-collapse" id="myNavbar">
@@ Line 38: / Line 67: @@
          </ul>
        </div>
        </div>
        </nav>
-       <div class="container-fluid">
+       <!-- <div class="container"> -->
-      <div class="jumbotron text-center row">
+      <div  id="sidebar" class="sidebar">
-      <h1>IISc Bangalore</h1>
-      <p>We are a group of undergraduates interested in synthetic biology.</p>
+        <a href="javascript:void(0)" class="closebtn" onclick="closeBar()">&times;</a>
+        <a class="nav" href="#overview">Contents</a>
+        <!-- <a class="nav" href="#design">Design</a> -->
+        <!-- <a class="nav" href="#protein">Protein</a> -->
+        <!-- <a class="nav" href="#aggre">Aggregation</a> -->
+        <a class="nav" href="#ref">References</a>
+        <a class="nav" href="#top">Back to top</a>
+            <a href="https://facebook.com/iiscbangaloreigem/" >
+          <svg class="ifacebook" enable-background="new 0 0 64 64" height="64px" id="Facebook" version="1.1" viewBox="0 0 64 64" width="64px" xml:space="preserve" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><linearGradient gradientTransform="matrix(1 0 0 -1 -822 -1026)" gradientUnits="userSpaceOnUse" id="Circle_Shadow_1_" x1="886.0303" x2="854.0302" y1="-1089.9985" y2="-1057.9985"><stop offset="0.3163" style="stop-color:#FFFFFF;stop-opacity:0"/><stop offset="0.3508" style="stop-color:#E9E9E9;stop-opacity:0.0504"/><stop offset="0.4543" style="stop-color:#ACACAC;stop-opacity:0.2018"/><stop offset="0.5572" style="stop-color:#787878;stop-opacity:0.3523"/><stop offset="0.6569" style="stop-color:#4D4D4D;stop-opacity:0.4981"/><stop offset="0.7528" style="stop-color:#2B2B2B;stop-opacity:0.6385"/><stop offset="0.8441" style="stop-color:#131313;stop-opacity:0.7719"/><stop offset="0.9286" style="stop-color:#050505;stop-opacity:0.8956"/><stop offset="1" style="stop-color:#000000"/></linearGradient><polygon enable-background="new    " fill="url(#Circle_Shadow_1_)" id="Circle_Shadow" opacity="0.2" points="64.029,64   64.029,30.059 49,15.029 15.059,48.971 30.088,64 "/><circle cx="32" cy="32" fill="#3A5BA0" id="Circle" r="24"/><g id="f_shadow"><g><defs><circle cx="32.029" cy="32" id="SVGID_1_" r="24"/></defs><clipPath id="SVGID_2_"><use overflow="visible" xlink:href="#SVGID_1_"/></clipPath><linearGradient gradientTransform="matrix(1 0 0 -1 -274 -299)" gradientUnits="userSpaceOnUse" id="SVGID_3_" x1="349.4473" x2="298.6123" y1="-367.8916" y2="-325.236"><stop offset="0.5816" style="stop-color:#000000;stop-opacity:0"/><stop offset="0.6306" style="stop-color:#000000;stop-opacity:0.117"/><stop offset="1" style="stop-color:#000000"/></linearGradient><path clip-path="url(#SVGID_2_)" d="M70.029,54h2v-5    c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.107,0v-0.106c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.104    c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.106    c0,0-0.041,0-0.106,0v-0.105c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0V47.83c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.105,0    v-0.105c0,0-0.041,0-0.106,0v-0.105c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.105    c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0V46.98c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.106,0v-0.106c0,0-0.04,0-0.106,0    v-0.105c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.105,0V46.45c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.104    c0,0-0.039,0-0.105,0V46.13c0,0-0.04,0-0.105,0v-0.106c0,0-0.041,0-0.106,0v-0.105c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0    v-0.106c0,0-0.04,0-0.106,0v-0.104c0,0-0.04,0-0.106,0v-0.107c0,0-0.039,0-0.104,0v-0.105c0,0-0.04,0-0.106,0v-0.105    c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0V45.07c0,0-0.041,0-0.106,0v-0.11c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0    v-0.105c0,0-0.04,0-0.105,0v-0.105c0,0-0.041,0-0.106,0v-0.105c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0v-0.106    c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.105,0V43.9c0,0-0.041,0-0.106,0    v-0.104c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.105,0v-0.105c0,0-0.041,0-0.106,0v-0.106    c0,0-0.04,0-0.106,0V43.26c0,0-0.04,0-0.105,0v-0.107c0,0-0.041,0-0.106,0v-0.104c0,0-0.04,0-0.106,0v-0.108c0,0-0.039,0-0.105,0    v-0.105c0,0-0.04,0-0.105,0v-0.105c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.104    c0,0-0.04,0-0.105,0v-0.106c0,0-0.041,0-0.106,0v-0.105c0,0-0.039,0-0.105,0V42.09c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0    v-0.106c0,0-0.04,0-0.107,0v-0.106c0,0-0.039,0-0.104,0v-0.104c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.105    c0,0-0.04,0-0.105,0V41.35c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.106,0    V40.92c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.104c0,0-0.041,0-0.106,0v-0.106    c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.105,0V40.18c0,0-0.041,0-0.106,0V40.07c0,0-0.04,0-0.106,0    v-0.107c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.106,0v-0.105c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0V39.54    c0,0-0.04,0-0.105,0v-0.106c0,0-0.041,0-0.106,0v-0.104c0,0-0.04,0-0.106,0v-0.108c0,0-0.039,0-0.105,0v-0.106    c0,0-0.039,0-0.105,0v-0.106c0,0-0.039,0-0.106,0V38.9c0,0-0.039,0-0.104,0v-0.106c0,0-0.041,0-0.107,0v-0.106    c0,0-0.039,0-0.105,0v-0.104c0,0-0.039,0-0.107,0v-0.106c0,0-0.038,0-0.104,0v-0.105c0,0-0.04,0-0.106,0v-0.105    c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0V38.05c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0    V37.73c0,0-0.04,0-0.105,0v-0.105c0,0-0.041,0-0.106,0v-0.107c0,0-0.04,0-0.106,0v-0.104c0,0-0.039,0-0.105,0v-0.106    c0,0-0.04,0-0.106,0v-0.105c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.105c0,0-0.041,0-0.106,0    v-0.106c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.105,0V36.46c0,0-0.041,0-0.106,0v-0.106    c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.106,0v-0.113c0,0-0.04,0-0.106,0v-0.105c0,0-0.038,0-0.104,0    v-0.105c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.104c0,0-0.04,0-0.105,0V35.5c0,0-0.041,0-0.106,0v-0.105    c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0V35.18c0,0-0.04,0-0.105,0v-0.105c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0    v-0.108c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.106,0v-0.105c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0v-0.105    c0,0-0.04,0-0.105,0v-0.106c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.105c0,0-0.039,0-0.105,0V34.01c0,0-0.04,0-0.105,0    v-0.105c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.104c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.106,0V33.48    c0,0-0.038,0-0.104,0v-0.105c0,0-0.04,0-0.106,0V33.27c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.107,0    v-0.105c0,0-0.039,0-0.104,0v-0.105c0,0-0.04,0-0.106,0V32.74c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.106    c0,0-0.041,0-0.106,0v-0.105c0,0-0.04,0-0.106,0V32.31c0,0-0.039,0-0.105,0v-0.107c0,0-0.04,0-0.106,0v-0.106c0,0-0.04,0-0.106,0    V31.99c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.107c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.106    c0,0-0.039,0-0.104,0V31.46c0,0-0.04,0-0.106,0v-0.107c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0V31.14c0,0-0.039,0-0.104,0    v-0.106c0,0-0.04,0-0.107,0v-0.106c0,0-0.04,0-0.106,0V30.82c0,0-0.039,0-0.105,0v-0.105c0,0-0.039,0-0.105,0v-0.106    c0,0-0.039,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.107c0,0-0.039,0-0.105,0V30.29c0,0-0.039,0-0.105,0v-0.106    c0,0-0.039,0-0.106,0v-0.106c0,0-0.04,0-0.106,0V29.97c0,0-0.039,0-0.105,0v-0.106c0,0-0.039,0-0.105,0v-0.106    c0,0-0.039,0-0.107,0v-0.106c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0V29.44c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.105,0    v-0.107c0,0-0.041,0-0.106,0V29.12c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.106    c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0V28.59c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.106,0v-0.107c0,0-0.04,0-0.106,0    v-0.106c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.105,0v-0.106c0,0-0.041,0-0.106,0V27.95c0,0-0.04,0-0.106,0v-0.106    c0,0-0.039,0-0.104,0v-0.106c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.107,0v-0.107    c0,0-0.039,0-0.104,0v-0.105c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0V27.1c0,0-0.04,0-0.107,0v-0.106c0,0-0.039,0-0.105,0    v-0.106c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.107c0,0-0.041,0-0.107,0v-0.106c0,0-0.039,0-0.105,0v-0.106    c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0V26.25c0,0-0.041,0-0.107,0v-0.106c0,0-0.039,0-0.105,0v-0.106    c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.106,0v-0.107c0,0-0.04,0-0.106,0V25.72c0,0-0.039,0-0.105,0v-0.106c0,0-0.039,0-0.105,0    v-0.106c0,0-0.039,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.107c0,0-0.039,0-0.105,0V25.19c0,0-0.039,0-0.105,0v-0.11    c0,0-0.039,0-0.106,0v-0.106c0,0-0.039,0-0.104,0v-0.106c0,0-0.041,0-0.107,0v-0.106c0,0-0.04,0-0.106,0v-0.106    c0,0-0.038,0-0.104,0V24.55c0,0-0.04,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0    v-0.106c0,0-0.041,0-0.106,0V24.02c0,0-0.04,0-0.106,0v-0.11c0,0-0.041,0-0.106,0v-0.106c0,0-0.039,0-0.104,0v-0.106    c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.107c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.106,0v-0.106c0,0-0.04,0-0.106,0    v-0.106c0,0-0.039,0-0.105,0V23.06c0,0-0.04,0-0.105,0v-0.106c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.106    c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0V22.53c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.107c0,0-0.039,0-0.105,0    v-0.106c0,0-0.04,0-0.106,0v-0.107c0,0-0.038,0-0.104,0v-0.105c0,0-0.04,0-0.107,0V21.89c0,0-0.039,0-0.105,0v-0.106    c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.106c0,0-0.041,0-0.106,0v-0.107c0,0-0.04,0-0.106,0v-0.106    c0,0-0.039,0-0.106,0v-0.106c0,0-0.039,0-0.104,0v-0.106c0,0-0.041,0-0.106,0V21.04c0,0-0.04,0-0.106,0v-0.106    c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0V20.51c0,0-0.04,0-0.105,0    v-0.106c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.106c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0V19.98    c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0v-0.107c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.106,0v-0.106c0,0-0.04,0-0.106,0    v-0.106c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.106c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0V19.02    c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.105,0v-0.106c0,0-0.041,0-0.106,0v-0.106c0,0-0.039,0-0.104,0v-0.106    c0,0-0.041,0-0.106,0V18.49c0,0-0.04,0-0.106,0v-0.107c0,0-0.039,0-0.105,0v-0.105c0,0-0.04,0-0.106,0v-0.107c0,0-0.04,0-0.106,0    v-0.106c0,0-0.039,0-0.105,0v-0.107c0,0-0.04,0-0.105,0v-0.106c0,0-0.041,0-0.106,0v-0.106c0,0-0.04,0-0.106,0V17.64    c0,0-0.039,0-0.105,0v-0.107c0,0-0.04,0-0.105,0v-0.105c0,0-0.041,0-0.106,0v-0.107c0,0-0.04,0-0.106,0v-0.106    c0,0-0.039,0-0.105,0v-0.106c0,0-0.04,0-0.106,0V17c0,0-2.05,0-4,0c-4.072,0-7,2.435-7,7v4h-5v5h0.106v0.105h0.106v0.106h0.107    v0.106h0.106v0.105h0.106v0.105h0.107v0.105h0.106v0.106h0.106v0.106h0.107v0.105h0.106v0.105h0.106v0.106h0.107v0.106h0.106    v0.104h0.106v0.106h0.107v0.105h0.105v0.105h0.106V34.8h0.107v0.106h0.106v0.104h0.106v0.107h0.106v0.104h0.106v0.106h0.106v0.106    h0.106v0.104h0.106v0.106h0.106v0.106h0.107v0.104h0.106v0.107h0.106v0.105h0.106v0.105h0.106v0.106h0.106v0.104h0.106v0.107    h0.106V36.6h0.107v0.105h0.106v0.107h0.106v0.104h0.107v0.106h0.106v0.105h0.105v0.105h0.106v0.106h0.107v0.104H28.7v0.107h0.107    v0.105h0.106v0.105h0.106v0.106H29V47h0.106v0.105h0.106v0.106h0.106v0.106h0.106v0.105h0.106v0.105h0.107v0.105h0.106v0.106    h0.106v0.106h0.106v0.105h0.106v0.105h0.107v0.106h0.106v0.106h0.106v0.104h0.106v0.106h0.106v0.105h0.106V48.7h0.107v0.106h0.106    v0.106h0.106v0.104h0.107v0.107h0.106v0.105h0.106v0.105h0.107v0.105h0.106v0.105h0.106v0.107h0.106v0.105h0.106v0.105h0.107    v0.107h0.106v0.104h0.106v0.106h0.106v0.105h0.106v0.105h0.107V50.5h0.106v0.107h0.105v0.104h0.106v0.106h0.106v0.105h0.105v0.105    h0.105v0.106h0.105v0.105h0.105v0.106h0.106v0.105h0.106v0.107h0.106v0.104h0.106v0.106h0.104v0.105h0.106v0.105h0.105v0.105H34.2    v0.105h0.106V52.3h0.106v0.106h0.104v0.105h0.106v0.106h0.106v0.104h0.105v0.107h0.105v0.105h0.106v0.105h0.106v0.105h0.105v0.106    h0.106v0.106h0.106v0.105h0.105v0.105h0.105v0.106h0.105v0.104h0.107v0.106h0.105v0.106h0.105V54.1h0.106v0.106h0.104v0.106h0.107    v0.104h0.106v0.106h0.104v0.105h0.106v0.105h0.106v0.106h0.105v0.106h0.107v0.105h0.104v0.105H37.3v0.105h0.106v0.106h0.105v0.106    h0.106v0.105h0.106v0.105h0.105v0.106h0.105V55.9h0.106v0.105h0.106v0.105h0.105v0.105h0.105v0.105h0.106v0.106h0.104v0.106h0.107    v0.105h0.106v0.106h0.106v0.104h0.104v0.106h0.106V57.1h0.106v0.106h0.106v0.106h0.104v0.104h0.106v0.106h0.105v0.105h0.105v0.107    h0.106v0.104h0.106v0.106h0.107v0.105h0.104v0.106h0.106v0.104h0.105v0.107h0.105v0.105h0.106v0.105h0.106v0.105h0.105v0.106    h0.105V58.9h0.106v0.105h0.106v0.105h0.105v0.105h0.106v0.107h0.106v0.105h0.105v0.105h0.105v0.105h0.106v0.106h0.106v0.104h0.104    v0.106h0.106v0.106h0.106v0.105h0.104v0.105h0.107v0.105h0.105v0.107H42.6v0.105h0.104V60.7h0.106v0.104h0.105v0.106h0.105v0.106    h0.106v0.105h0.106v0.105h0.105v0.105h0.107v0.107h0.104v0.104h0.104v0.106h0.107v0.105h0.106v0.105h0.106v0.106h0.105v0.106    h0.105v0.105h0.106v0.105H44.4v0.107h0.104v0.104h0.106v0.106h0.106v0.105h0.106v0.105h0.104v0.106h0.107v0.104h0.104v0.107h0.106    v0.105h0.106v0.105h0.106v0.106h0.14v0.106h0.107v0.105h0.105v0.105h0.105v0.105h0.105v0.105h0.106v0.106h0.106v0.106h0.105v0.105    h0.106v0.105h0.106v0.106h0.105v0.105h0.105v0.106h0.106v0.105h0.106v0.105h0.105v0.106h0.105v0.106h0.106v0.104h0.106v0.106    h0.105v0.105h0.105v0.107h0.106v0.104h0.105v0.106h0.106v0.105h0.105v0.105h0.105V66.1h0.107v0.104h0.105v0.106h0.105v0.105h0.107    v0.105h0.104v0.106h0.106v0.106h0.105v0.105h0.106v0.105h0.106v0.105h0.105v0.106h0.105v0.106h0.106v0.105h0.106v0.105h0.105    v0.106h0.105v0.104h0.106v0.107h0.106v0.105h0.104v0.105h0.106v0.105h0.107v0.106h0.105v0.106h0.105v0.105h0.106v0.105h0.104    v0.105h0.107v0.106h0.105V68.9h0.105v0.105h0.107v0.105h0.105v0.106h0.105v0.106h0.106v0.105h0.105v0.105h0.106v0.105h0.105v0.106    h0.105v0.106h0.106v0.104h0.106v0.106h0.105v0.105h0.105v0.107h0.106v0.104h0.106v0.106h0.105v0.105h0.105V70.7h0.107v0.104h0.104    v0.106h0.106v0.106h0.106v0.105h0.106v0.105h0.105v0.106h0.105v0.106h0.105v0.105h0.105v0.106h0.106v0.104h0.106v0.106h0.106    v0.106h0.106v0.105h0.105v0.105h0.105v0.106h0.105v0.104h0.106V72.5h0.106v0.105h0.105v0.105h0.105v0.105h0.106v0.106h0.106v0.106    h0.105v0.105h0.105v0.105h0.107v0.105h0.105v0.106h0.105v0.106h0.106v0.105h0.106v0.105h0.105v0.106h0.105v0.106h0.106v0.104    h0.106v0.106h0.105v0.105h0.105v0.106h0.105v0.106h0.106v0.104h0.106v0.106h0.106v0.105h0.106v0.105h0.104v0.106h0.106v0.106    h0.105v0.105h0.105v0.106h0.106v0.104h0.106v0.106h0.105v0.106h0.106v0.105h0.106v0.105h0.105v0.106h0.105V76.1h0.106v0.105h0.106    v0.105h0.105v0.105h0.105v0.106h0.106v0.106h0.106v0.105h0.105v0.105h0.105v0.106h0.107v0.104h0.105v0.106h0.105v0.106h0.105    v0.105h0.105v0.105h0.107v0.106h0.105v0.104H59.8v0.107h0.104v0.105h0.106v0.105h0.106v0.106h0.105v0.106h0.106v0.105h0.106v0.105    h0.105v0.105h0.105v0.105h0.106v0.106h0.106v0.106h0.105V79h6V65h4l1-5h-0.127l0.021-0.105H71.73l0.021-0.106h-0.128l0.021-0.104    h-0.129l0.021-0.106h-0.129l0.021-0.106h-0.128l0.021-0.105h-0.127l0.021-0.106h-0.128l0.021-0.106h-0.06l0.021-0.105h-0.127    l0.021-0.105h-0.128l0.021-0.105H70.73l0.021-0.105h-0.128l0.021-0.106h-0.129l0.021-0.106h-0.128l0.021-0.105h-0.127l0.021-0.105    h-0.128l0.021-0.106h-0.128l0.021-0.106H69.98l0.021-0.104H69.88l0.021-0.106h-0.127l0.021-0.105h-0.128l0.021-0.106h-0.129    l0.021-0.106h-0.128l0.021-0.105h-0.127l0.021-0.105h-0.128l0.021-0.106h-0.128l0.021-0.104h-0.129l0.021-0.106h-0.128    l0.021-0.106h-0.127l0.021-0.105h-0.128l0.021-0.106h-0.129l0.021-0.104h-0.128l0.021-0.107h-0.127l0.021-0.105h-0.129    l0.021-0.105h-0.127l0.021-0.105h-0.127l0.021-0.106h-0.128l0.021-0.106h-0.129l0.021-0.105h-0.128l0.021-0.105h-0.127    l0.021-0.105h-0.128l0.021-0.105H67.42l0.021-0.106h-0.129l0.021-0.106h-0.113l0.021-0.105h-0.104    C67.407,54.305,68.253,54,70.029,54z" enable-background="new    " fill="url(#SVGID_3_)" opacity="0.5"/></g></g><path d="M39,33l1-5l-5,0.001V24c0-1.544,0.784-2,3-2h2v-5c0,0-2.05,0-4,0c-4.072,0-7,2.435-7,7v4h-5v5h5  v14h6V33H39z" fill="#FFFFFF" id="f_1_"/>
+  </svg></a>
+  <a href="https://twitter.com/IISc_iGEM">
+  <svg class="itwitter" enable-background="new 0 0 64 64" height="64px" id="Tweet_Tweet" version="1.1" viewBox="0 0 64 64" width="64px" xml:space="preserve" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><linearGradient gradientTransform="matrix(1 0 0 1 -2192 -2908)" gradientUnits="userSpaceOnUse" id="Circle_Shadow_1_" x1="2256.0293" x2="2224.03" y1="2972" y2="2940.0007"><stop offset="0.3163" style="stop-color:#FFFFFF;stop-opacity:0"/><stop offset="0.3508" style="stop-color:#E9E9E9;stop-opacity:0.0504"/><stop offset="0.4543" style="stop-color:#ACACAC;stop-opacity:0.2018"/><stop offset="0.5572" style="stop-color:#787878;stop-opacity:0.3523"/><stop offset="0.6569" style="stop-color:#4D4D4D;stop-opacity:0.4981"/><stop offset="0.7528" style="stop-color:#2B2B2B;stop-opacity:0.6385"/><stop offset="0.8441" style="stop-color:#131313;stop-opacity:0.7719"/><stop offset="0.9286" style="stop-color:#050505;stop-opacity:0.8956"/><stop offset="1" style="stop-color:#000000"/></linearGradient><polygon enable-background="new    " fill="url(#Circle_Shadow_1_)" id="Circle_Shadow" opacity="0.2" points="64.029,64   64.029,30.059 49,15.029 15.059,48.971 30.088,64 "/><circle cx="32" cy="32" fill="#2CA7E0" id="Circle" r="24"/><g id="Bird_shadow"><g><defs><circle cx="32" cy="32" id="SVGID_1_" r="24"/></defs><clipPath id="SVGID_2_"><use overflow="visible" xlink:href="#SVGID_1_"/></clipPath><linearGradient gradientTransform="matrix(1 0 0 -1 -274 -299)" gradientUnits="userSpaceOnUse" id="SVGID_3_" x1="345.5957" x2="294.4228" y1="-370.2207" y2="-319.0478"><stop offset="0.4082" style="stop-color:#000000;stop-opacity:0"/><stop offset="0.6888" style="stop-color:#00005E"/></linearGradient><path clip-path="url(#SVGID_2_)" d="M78.971,55.209    c-0.078,0.035-0.177,0.074-0.262,0.109c0.052-0.072,0.105-0.144,0.154-0.217c-0.078,0.035-0.177,0.074-0.261,0.11    c0.052-0.073,0.104-0.144,0.154-0.217c-0.078,0.034-0.177,0.073-0.261,0.11c0.051-0.073,0.104-0.145,0.154-0.217    c-0.078,0.034-0.177,0.072-0.261,0.108c0.05-0.072,0.104-0.144,0.153-0.215c-0.078,0.034-0.178,0.073-0.26,0.11    c0.049-0.074,0.104-0.146,0.153-0.219c-0.077,0.035-0.177,0.074-0.26,0.11c0.05-0.072,0.104-0.144,0.153-0.216    c-0.08,0.035-0.178,0.074-0.262,0.109c0.051-0.072,0.105-0.144,0.154-0.217c-0.078,0.035-0.177,0.074-0.261,0.109    c0.05-0.072,0.104-0.144,0.153-0.217c-0.078,0.035-0.177,0.074-0.261,0.11c0.052-0.073,0.104-0.144,0.154-0.217    c-0.078,0.035-0.177,0.073-0.261,0.11c0.051-0.073,0.104-0.145,0.154-0.217c-0.078,0.034-0.177,0.072-0.261,0.109    c0.052-0.073,0.104-0.145,0.154-0.217c-0.079,0.035-0.177,0.074-0.261,0.109c0.049-0.072,0.104-0.143,0.152-0.216    c-0.078,0.035-0.176,0.073-0.26,0.109c0.051-0.072,0.105-0.144,0.154-0.216c-0.08,0.035-0.178,0.074-0.262,0.109    c0.051-0.072,0.105-0.144,0.154-0.217c-0.021,0.01-0.047,0.02-0.068,0.028c0.234-0.425,0.43-0.851,0.558-1.249    c-0.062,0.035-0.128,0.078-0.19,0.117c0.027-0.074,0.061-0.151,0.084-0.225c-0.062,0.036-0.128,0.079-0.189,0.117    c0.027-0.074,0.061-0.15,0.084-0.225c-0.063,0.037-0.129,0.08-0.19,0.117c0.027-0.074,0.062-0.149,0.084-0.223    c-0.062,0.036-0.127,0.078-0.188,0.116c0.027-0.074,0.061-0.149,0.084-0.224c-0.063,0.037-0.129,0.08-0.19,0.117    c0.026-0.074,0.062-0.15,0.084-0.224c-0.062,0.036-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.151,0.084-0.224    c-0.062,0.035-0.127,0.078-0.189,0.116c0.027-0.073,0.061-0.151,0.084-0.224c-0.063,0.036-0.128,0.079-0.189,0.117    c0.026-0.074,0.061-0.151,0.084-0.225c-0.063,0.036-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.15,0.084-0.224    c-0.062,0.036-0.128,0.079-0.19,0.116c0.027-0.073,0.062-0.15,0.084-0.224c-0.062,0.036-0.127,0.078-0.188,0.116    c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.035-0.128,0.078-0.19,0.117c0.027-0.074,0.061-0.15,0.084-0.224    c-0.062,0.036-0.128,0.079-0.189,0.117c0.027-0.074,0.061-0.151,0.084-0.224c-0.063,0.035-0.129,0.078-0.19,0.117    c0.027-0.074,0.062-0.152,0.084-0.225c-0.062,0.036-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.151,0.084-0.225    c-0.062,0.036-0.127,0.08-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.224c-0.063,0.036-0.128,0.079-0.189,0.116    c0.026-0.073,0.061-0.149,0.084-0.223c-0.063,0.036-0.129,0.078-0.189,0.116c0.027-0.073,0.061-0.149,0.084-0.224    c-0.063,0.037-0.129,0.08-0.191,0.117c0.027-0.074,0.063-0.15,0.086-0.225c-0.063,0.036-0.129,0.079-0.19,0.117    c0.026-0.074,0.062-0.15,0.084-0.225c-0.062,0.037-0.128,0.08-0.19,0.117c0.027-0.074,0.062-0.149,0.084-0.223    c-0.062,0.036-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.151,0.084-0.225c-0.063,0.037-0.128,0.08-0.189,0.117    c0.026-0.074,0.061-0.15,0.084-0.224c-0.063,0.036-0.129,0.079-0.191,0.116c0.027-0.073,0.062-0.15,0.085-0.223    c-0.063,0.035-0.128,0.077-0.189,0.115c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.035-0.128,0.078-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.224c-0.062,0.037-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.149,0.084-0.224    c-0.062,0.035-0.127,0.078-0.188,0.115c0.026-0.074,0.061-0.149,0.084-0.223c-0.063,0.036-0.129,0.079-0.19,0.117    c0.026-0.074,0.062-0.151,0.084-0.225c-0.062,0.037-0.128,0.08-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.224    c-0.062,0.036-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.151,0.084-0.224c-0.063,0.035-0.128,0.078-0.189,0.116    c0.026-0.073,0.061-0.151,0.084-0.224c-0.063,0.035-0.127,0.078-0.189,0.115c0.027-0.073,0.061-0.149,0.084-0.223    c-0.063,0.037-0.129,0.079-0.191,0.118c0.026-0.075,0.062-0.151,0.084-0.226c-0.061,0.037-0.127,0.078-0.188,0.117    c0.026-0.074,0.061-0.149,0.084-0.223c-0.062,0.036-0.128,0.079-0.19,0.117c0.027-0.074,0.061-0.15,0.084-0.225    c-0.062,0.037-0.128,0.08-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.224c-0.063,0.036-0.129,0.079-0.19,0.117    c0.027-0.074,0.062-0.151,0.084-0.224c-0.062,0.035-0.128,0.078-0.19,0.116c0.027-0.073,0.062-0.151,0.084-0.224    c-0.061,0.035-0.127,0.078-0.188,0.115c0.026-0.072,0.062-0.148,0.084-0.221c-0.062,0.036-0.129,0.078-0.19,0.116    c0.025-0.073,0.061-0.149,0.084-0.224c-0.062,0.035-0.127,0.078-0.189,0.115c0.027-0.073,0.061-0.149,0.084-0.223    c-0.063,0.036-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.225c-0.063,0.037-0.129,0.08-0.19,0.117    c0.026-0.074,0.062-0.15,0.084-0.224c-0.062,0.036-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.151,0.084-0.224    c-0.062,0.035-0.128,0.078-0.189,0.117c0.027-0.074,0.061-0.152,0.084-0.225c-0.061,0.036-0.127,0.078-0.189,0.115    c0.027-0.073,0.061-0.149,0.084-0.223c-0.062,0.037-0.129,0.079-0.191,0.118c0.027-0.075,0.062-0.151,0.084-0.225    c-0.061,0.036-0.127,0.077-0.188,0.116c0.027-0.073,0.062-0.149,0.084-0.223c-0.062,0.036-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.225c-0.062,0.037-0.127,0.08-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.224    c-0.063,0.036-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.151,0.084-0.224c-0.063,0.035-0.129,0.078-0.19,0.117    c0.026-0.074,0.062-0.152,0.084-0.225c-0.062,0.036-0.127,0.078-0.188,0.116c0.026-0.074,0.061-0.149,0.084-0.222    c-0.063,0.037-0.129,0.078-0.191,0.116c0.027-0.073,0.061-0.151,0.084-0.224c-0.061,0.035-0.127,0.078-0.189,0.115    c0.027-0.073,0.062-0.149,0.084-0.223c-0.062,0.037-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.15,0.085-0.225    c-0.063,0.035-0.128,0.078-0.19,0.117c0.027-0.074,0.062-0.15,0.085-0.224c-0.063,0.036-0.129,0.079-0.191,0.117    c0.027-0.074,0.062-0.151,0.084-0.224c-0.062,0.035-0.127,0.078-0.189,0.117c0.027-0.074,0.061-0.151,0.084-0.225    c-0.061,0.036-0.127,0.078-0.189,0.116c0.027-0.074,0.062-0.15,0.084-0.224c-0.062,0.037-0.129,0.08-0.191,0.119    c0.027-0.076,0.063-0.152,0.085-0.226c-0.062,0.036-0.128,0.079-0.19,0.116c0.027-0.073,0.062-0.149,0.084-0.223    c-0.062,0.036-0.127,0.078-0.188,0.116c0.026-0.073,0.062-0.149,0.084-0.223c-0.062,0.036-0.128,0.079-0.19,0.116    c0.027-0.073,0.062-0.15,0.084-0.224c-0.062,0.036-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.151,0.084-0.224    c-0.063,0.035-0.128,0.078-0.189,0.117c0.026-0.074,0.061-0.151,0.084-0.225c-0.062,0.036-0.127,0.078-0.189,0.116    c0.027-0.074,0.061-0.149,0.084-0.222c-0.063,0.037-0.129,0.078-0.192,0.117c0.027-0.074,0.062-0.15,0.085-0.225    c-0.062,0.036-0.128,0.079-0.191,0.117c0.027-0.074,0.063-0.151,0.086-0.225c-0.063,0.035-0.129,0.078-0.189,0.115    c0.027-0.074,0.061-0.15,0.084-0.223c-0.063,0.035-0.128,0.078-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.063,0.035-0.129,0.078-0.19,0.116c0.027-0.073,0.062-0.151,0.084-0.224c-0.062,0.035-0.128,0.078-0.19,0.117    c0.027-0.074,0.062-0.151,0.084-0.225c-0.061,0.036-0.127,0.078-0.188,0.116c0.026-0.074,0.062-0.149,0.084-0.224    c-0.062,0.037-0.129,0.08-0.192,0.117c0.027-0.074,0.063-0.15,0.086-0.224c-0.063,0.036-0.129,0.079-0.19,0.116    c0.026-0.073,0.062-0.149,0.084-0.223c-0.062,0.037-0.128,0.078-0.188,0.116c0.026-0.073,0.061-0.149,0.084-0.223    c-0.063,0.036-0.129,0.079-0.19,0.116c0.026-0.073,0.062-0.15,0.084-0.223c-0.062,0.035-0.128,0.078-0.19,0.116    c0.027-0.073,0.062-0.151,0.084-0.224c-0.062,0.035-0.128,0.078-0.189,0.117c0.027-0.074,0.061-0.151,0.084-0.225    c-0.063,0.036-0.129,0.079-0.19,0.116c0.027-0.073,0.062-0.149,0.084-0.222c-0.062,0.035-0.128,0.078-0.19,0.117    c0.027-0.076,0.062-0.151,0.084-0.226c-0.062,0.036-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.151,0.084-0.224    c-0.063,0.035-0.128,0.078-0.189,0.115c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.036-0.127,0.079-0.189,0.115    c0.027-0.072,0.061-0.148,0.084-0.221c-0.063,0.035-0.128,0.078-0.189,0.116c0.026-0.073,0.061-0.151,0.084-0.224    c-0.063,0.035-0.129,0.078-0.19,0.117c0.026-0.074,0.062-0.151,0.084-0.225c-0.062,0.036-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.225c-0.062,0.037-0.127,0.08-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.224    c-0.063,0.036-0.128,0.079-0.189,0.116c0.026-0.073,0.061-0.148,0.084-0.223c-0.063,0.037-0.127,0.078-0.189,0.117    c0.027-0.074,0.061-0.15,0.084-0.224c-0.063,0.036-0.129,0.079-0.19,0.116c0.027-0.073,0.062-0.15,0.084-0.223    c-0.062,0.037-0.129,0.078-0.19,0.116c0.027-0.073,0.061-0.149,0.084-0.224c-0.062,0.036-0.127,0.078-0.189,0.115    c0.027-0.073,0.061-0.149,0.084-0.223c-0.063,0.036-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.224    c-0.063,0.036-0.129,0.079-0.19,0.116c0.026-0.073,0.062-0.15,0.084-0.224c-0.062,0.036-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.151,0.084-0.224c-0.062,0.035-0.127,0.078-0.188,0.115c0.026-0.074,0.062-0.149,0.084-0.223    c-0.062,0.036-0.128,0.079-0.19,0.116c0.027-0.073,0.062-0.149,0.084-0.222c-0.062,0.037-0.129,0.078-0.19,0.117    c0.026-0.074,0.062-0.15,0.084-0.225c-0.062,0.036-0.127,0.078-0.188,0.116c0.026-0.074,0.061-0.15,0.084-0.224    c-0.063,0.036-0.129,0.08-0.19,0.117c0.026-0.074,0.061-0.15,0.084-0.224c-0.062,0.036-0.128,0.079-0.19,0.116    c0.027-0.073,0.061-0.15,0.084-0.224c-0.062,0.036-0.128,0.079-0.189,0.116c0.027-0.072,0.061-0.148,0.084-0.223    c-0.063,0.037-0.129,0.078-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.224c-0.063,0.036-0.128,0.079-0.189,0.116    c0.027-0.073,0.061-0.15,0.084-0.223c-0.063,0.037-0.129,0.078-0.192,0.117c0.026-0.074,0.062-0.151,0.085-0.226    c-0.061,0.036-0.127,0.078-0.189,0.116c0.027-0.073,0.062-0.149,0.084-0.223c-0.062,0.037-0.127,0.08-0.189,0.117    c0.027-0.074,0.061-0.15,0.084-0.224c-0.063,0.036-0.128,0.079-0.189,0.116c0.026-0.073,0.061-0.15,0.084-0.223    c-0.063,0.035-0.129,0.078-0.19,0.116c0.026-0.073,0.062-0.151,0.084-0.224c-0.062,0.035-0.128,0.078-0.19,0.117    c0.027-0.074,0.062-0.151,0.084-0.225c-0.061,0.036-0.127,0.078-0.188,0.116c0.026-0.074,0.062-0.149,0.084-0.222    c-0.062,0.037-0.13,0.078-0.191,0.117c0.027-0.074,0.062-0.15,0.084-0.225c-0.061,0.036-0.127,0.078-0.188,0.116    c0.027-0.074,0.061-0.15,0.084-0.224c-0.063,0.037-0.129,0.08-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.224    c-0.062,0.036-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.151,0.084-0.224c-0.062,0.035-0.127,0.078-0.189,0.115    c0.027-0.072,0.061-0.148,0.084-0.222c-0.063,0.036-0.128,0.079-0.189,0.116c0.026-0.073,0.061-0.15,0.084-0.224    c-0.063,0.036-0.129,0.078-0.189,0.116c0.027-0.074,0.061-0.15,0.084-0.225c-0.063,0.037-0.129,0.08-0.191,0.117    c0.027-0.074,0.062-0.15,0.084-0.224c-0.061,0.036-0.127,0.078-0.188,0.116c0.026-0.073,0.062-0.148,0.084-0.223    c-0.062,0.037-0.128,0.08-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.224c-0.062,0.036-0.127,0.079-0.189,0.117    c0.027-0.074,0.061-0.151,0.084-0.224c-0.063,0.035-0.128,0.078-0.189,0.116c0.026-0.073,0.061-0.151,0.084-0.224    c-0.063,0.035-0.129,0.079-0.191,0.117c0.027-0.074,0.062-0.151,0.085-0.225c-0.062,0.036-0.128,0.078-0.189,0.116    c0.027-0.073,0.062-0.149,0.084-0.222c-0.062,0.037-0.129,0.078-0.19,0.117c0.027-0.074,0.061-0.151,0.084-0.225    c-0.062,0.036-0.127,0.078-0.189,0.116c0.027-0.074,0.061-0.149,0.084-0.224c-0.063,0.037-0.128,0.08-0.189,0.117    c0.026-0.074,0.061-0.15,0.084-0.224c-0.063,0.036-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.151,0.084-0.224    c-0.062,0.035-0.128,0.078-0.19,0.117c0.027-0.074,0.062-0.152,0.084-0.225c-0.062,0.036-0.127,0.079-0.189,0.117    c0.027-0.074,0.061-0.151,0.084-0.225c-0.061,0.036-0.127,0.078-0.189,0.116c0.027-0.073,0.062-0.149,0.084-0.223    c-0.062,0.036-0.129,0.079-0.19,0.116c0.026-0.073,0.062-0.15,0.084-0.224c-0.062,0.036-0.127,0.078-0.188,0.116    c0.026-0.074,0.061-0.148,0.084-0.223c-0.063,0.037-0.129,0.08-0.19,0.117c0.026-0.074,0.061-0.15,0.084-0.224    c-0.062,0.036-0.128,0.079-0.19,0.117c0.027-0.074,0.061-0.151,0.084-0.224c-0.062,0.035-0.128,0.078-0.189,0.117    c0.027-0.074,0.061-0.152,0.084-0.225c-0.063,0.036-0.129,0.079-0.19,0.117c0.027-0.074,0.062-0.151,0.084-0.225    c-0.062,0.037-0.127,0.078-0.188,0.116c0.027-0.073,0.061-0.149,0.084-0.222c-0.063,0.037-0.129,0.079-0.191,0.117    c0.027-0.074,0.061-0.151,0.084-0.225c-0.061,0.036-0.127,0.078-0.189,0.116c0.027-0.073,0.062-0.149,0.084-0.223    c-0.062,0.036-0.127,0.079-0.189,0.116c0.027-0.073,0.061-0.15,0.084-0.224c-0.063,0.036-0.128,0.079-0.189,0.117    c0.026-0.074,0.061-0.151,0.084-0.224c-0.063,0.035-0.129,0.078-0.19,0.117c0.026-0.074,0.062-0.151,0.084-0.225    c-0.062,0.036-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.151,0.084-0.225c-0.061,0.037-0.127,0.078-0.188,0.116    c0.026-0.073,0.062-0.149,0.084-0.223c-0.062,0.037-0.129,0.079-0.192,0.118c0.027-0.075,0.063-0.151,0.086-0.225    c-0.063,0.035-0.129,0.078-0.189,0.115c0.027-0.074,0.061-0.15,0.084-0.223c-0.063,0.035-0.129,0.078-0.19,0.115    c0.027-0.072,0.062-0.148,0.084-0.222c-0.062,0.036-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.151,0.084-0.224    c-0.062,0.035-0.127,0.078-0.189,0.117c0.027-0.074,0.061-0.151,0.084-0.225c-0.063,0.036-0.128,0.079-0.189,0.117    c0.026-0.074,0.061-0.15,0.084-0.225c-0.063,0.037-0.129,0.08-0.19,0.117c0.026-0.074,0.062-0.15,0.084-0.224    c-0.062,0.036-0.127,0.078-0.188,0.116c0.026-0.074,0.061-0.15,0.084-0.223c-0.063,0.035-0.129,0.078-0.19,0.116    c0.026-0.073,0.062-0.149,0.084-0.223c-0.062,0.036-0.129,0.079-0.19,0.116c0.026-0.073,0.062-0.15,0.084-0.225    c-0.062,0.036-0.127,0.079-0.189,0.116c0.027-0.073,0.061-0.149,0.084-0.222c-0.063,0.035-0.128,0.078-0.189,0.115    c0.026-0.072,0.061-0.148,0.084-0.222c-0.063,0.036-0.129,0.079-0.191,0.117c0.027-0.074,0.063-0.151,0.085-0.226    c-0.062,0.037-0.128,0.08-0.19,0.117c0.027-0.074,0.061-0.15,0.084-0.224c-0.062,0.036-0.128,0.079-0.189,0.117    c0.027-0.074,0.061-0.151,0.084-0.224c-0.061,0.035-0.127,0.078-0.189,0.115c0.027-0.074,0.061-0.148,0.084-0.222    c-0.063,0.036-0.128,0.079-0.189,0.116c0.025-0.073,0.061-0.15,0.084-0.223c-0.063,0.035-0.129,0.078-0.19,0.116    c0.027-0.073,0.062-0.151,0.084-0.224c-0.062,0.035-0.128,0.078-0.19,0.117c0.027-0.074,0.062-0.151,0.084-0.225    c-0.062,0.036-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.225c-0.063,0.037-0.128,0.08-0.189,0.117    c0.026-0.074,0.061-0.15,0.084-0.224c-0.063,0.036-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.151,0.084-0.224    c-0.062,0.035-0.127,0.078-0.188,0.115c0.026-0.074,0.061-0.148,0.084-0.222c-0.063,0.036-0.129,0.079-0.19,0.117    c0.026-0.074,0.062-0.151,0.084-0.224c-0.062,0.035-0.128,0.078-0.19,0.116c0.027-0.073,0.062-0.151,0.084-0.224    c-0.062,0.037-0.129,0.078-0.19,0.117c0.026-0.074,0.061-0.15,0.084-0.225c-0.062,0.036-0.128,0.078-0.189,0.116    c0.027-0.073,0.062-0.149,0.085-0.224c-0.062,0.037-0.128,0.078-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.224    c-0.063,0.037-0.129,0.079-0.192,0.118c0.027-0.075,0.062-0.15,0.085-0.225c-0.063,0.037-0.128,0.078-0.189,0.117    c0.027-0.074,0.061-0.151,0.084-0.225c-0.063,0.036-0.129,0.078-0.189,0.116c0.027-0.074,0.061-0.15,0.084-0.224    c-0.063,0.037-0.128,0.08-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.223c-0.063,0.036-0.129,0.079-0.19,0.117    c0.026-0.074,0.062-0.151,0.084-0.225c-0.062,0.036-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.224    c-0.062,0.036-0.127,0.079-0.189,0.116c0.027-0.073,0.061-0.15,0.084-0.224c-0.063,0.036-0.128,0.079-0.189,0.117    c0.026-0.074,0.061-0.151,0.084-0.224c-0.062,0.035-0.127,0.078-0.189,0.115c0.027-0.072,0.061-0.148,0.084-0.222    c-0.063,0.037-0.129,0.079-0.191,0.118c0.026-0.075,0.062-0.152,0.084-0.227c-0.061,0.037-0.127,0.078-0.188,0.117    c0.026-0.074,0.061-0.15,0.084-0.223c-0.062,0.036-0.128,0.079-0.19,0.117c0.027-0.074,0.061-0.151,0.084-0.224    c-0.062,0.037-0.128,0.079-0.189,0.117c0.027-0.075,0.061-0.151,0.084-0.224c-0.063,0.037-0.129,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223    c-0.062,0.037-0.127,0.079-0.188,0.116c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.129,0.079-0.19,0.117    c0.026-0.075,0.062-0.151,0.084-0.224c-0.062,0.037-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.15,0.084-0.223    c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223c-0.061,0.036-0.127,0.079-0.188,0.116    c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223    c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223c-0.063,0.036-0.127,0.079-0.189,0.116    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.222    c-0.063,0.037-0.129,0.08-0.192,0.118c0.027-0.075,0.062-0.152,0.085-0.225c-0.063,0.037-0.128,0.079-0.189,0.117    c0.027-0.074,0.061-0.15,0.084-0.223c-0.062,0.036-0.127,0.079-0.189,0.116c0.027-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.223c-0.063,0.037-0.129,0.079-0.19,0.117    c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223    c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223c-0.061,0.036-0.127,0.078-0.189,0.116    c0.027-0.073,0.062-0.149,0.084-0.221c-0.062,0.037-0.129,0.079-0.19,0.118c0.026-0.075,0.062-0.151,0.084-0.224    c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.075,0.063-0.151,0.086-0.224c-0.063,0.037-0.129,0.079-0.19,0.117    c0.026-0.074,0.062-0.15,0.084-0.222c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.062,0.037-0.128,0.079-0.189,0.117c0.027-0.074,0.061-0.151,0.084-0.223c-0.063,0.037-0.129,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223    c-0.061,0.036-0.127,0.078-0.188,0.116c0.027-0.073,0.062-0.149,0.084-0.222c-0.062,0.037-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.129,0.079-0.19,0.117c0.026-0.075,0.062-0.151,0.085-0.224    c-0.063,0.037-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.223c-0.062,0.036-0.127,0.078-0.189,0.116    c0.027-0.074,0.061-0.15,0.084-0.222c-0.063,0.037-0.129,0.08-0.191,0.118c0.026-0.075,0.062-0.151,0.084-0.225    c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223c-0.063,0.037-0.128,0.079-0.189,0.117    c0.026-0.074,0.061-0.15,0.084-0.223c-0.062,0.036-0.127,0.079-0.189,0.116c0.027-0.074,0.061-0.15,0.084-0.222    c-0.063,0.037-0.129,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.223c-0.063,0.037-0.129,0.079-0.19,0.117    c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223    c-0.061,0.036-0.127,0.079-0.188,0.116c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.223c-0.063,0.037-0.129,0.079-0.19,0.117    c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223    c-0.062,0.037-0.128,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223c-0.063,0.036-0.127,0.079-0.189,0.116    c0.027-0.074,0.061-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.129,0.079-0.19,0.117c0.027-0.074,0.062-0.151,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.128,0.079-0.191,0.117c0.027-0.074,0.063-0.151,0.086-0.224c-0.063,0.037-0.129,0.079-0.19,0.117    c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.036-0.127,0.078-0.188,0.116c0.026-0.073,0.061-0.149,0.084-0.221    c-0.063,0.037-0.129,0.079-0.191,0.117c0.027-0.075,0.063-0.151,0.085-0.224c-0.062,0.037-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.151,0.084-0.224c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.222    c-0.063,0.037-0.128,0.079-0.189,0.117c0.025-0.075,0.061-0.151,0.084-0.224c-0.063,0.037-0.129,0.079-0.191,0.117    c0.027-0.074,0.062-0.151,0.084-0.224c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.128,0.079-0.189,0.117c0.025-0.074,0.061-0.151,0.084-0.224c-0.062,0.036-0.127,0.078-0.189,0.116    c0.027-0.074,0.061-0.15,0.084-0.222c-0.063,0.037-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.151,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.151,0.084-0.224c-0.063,0.037-0.129,0.079-0.19,0.117    c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223    c-0.061,0.036-0.127,0.078-0.188,0.116c0.026-0.074,0.062-0.15,0.084-0.222c-0.062,0.037-0.128,0.079-0.19,0.117    c0.027-0.074,0.061-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.189,0.117c0.027-0.074,0.061-0.151,0.084-0.223    c-0.063,0.037-0.129,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.128,0.079-0.189,0.117c0.025-0.075,0.061-0.151,0.084-0.224c-0.062,0.036-0.127,0.079-0.189,0.116    c0.027-0.074,0.061-0.15,0.084-0.222c-0.063,0.037-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.127,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.061,0.036-0.127,0.078-0.189,0.116c0.027-0.074,0.062-0.15,0.084-0.222c-0.062,0.037-0.127,0.079-0.19,0.117    c0.026-0.074,0.062-0.151,0.085-0.224c-0.063,0.037-0.129,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223    c-0.062,0.037-0.129,0.079-0.19,0.118c0.027-0.074,0.061-0.151,0.084-0.224c-0.062,0.036-0.127,0.078-0.189,0.116    c0.027-0.074,0.061-0.15,0.084-0.223c-0.063,0.037-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.036-0.127,0.078-0.188,0.116    c0.026-0.074,0.061-0.15,0.084-0.222c-0.063,0.037-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.151,0.084-0.224    c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.129,0.079-0.191,0.118    c0.027-0.075,0.063-0.152,0.085-0.225c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.151,0.084-0.224    c-0.061,0.036-0.127,0.079-0.188,0.116c0.026-0.074,0.062-0.15,0.084-0.222c-0.062,0.037-0.128,0.079-0.19,0.117    c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.189,0.117c0.027-0.074,0.061-0.15,0.084-0.223    c-0.061,0.036-0.127,0.078-0.189,0.116c0.027-0.074,0.061-0.15,0.084-0.222c-0.062,0.037-0.128,0.079-0.189,0.117    c0.025-0.075,0.061-0.151,0.084-0.224c-0.063,0.037-0.129,0.079-0.191,0.118c0.027-0.074,0.061-0.151,0.084-0.224    c-0.061,0.036-0.127,0.078-0.189,0.116c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.127,0.079-0.19,0.117    c0.026-0.075,0.062-0.151,0.085-0.224c-0.063,0.037-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.222    c-0.063,0.037-0.129,0.079-0.19,0.117c0.026-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.128,0.079-0.19,0.117    c0.026-0.075,0.062-0.151,0.084-0.224c-0.061,0.036-0.127,0.078-0.188,0.116c0.026-0.073,0.062-0.149,0.084-0.221    c-0.062,0.037-0.128,0.079-0.19,0.117c0.027-0.074,0.062-0.15,0.084-0.223c-0.062,0.037-0.127,0.079-0.189,0.117    c0.027-0.075,0.061-0.151,0.084-0.224c-0.063,0.037-0.128,0.079-0.189,0.117c0.026-0.074,0.061-0.15,0.084-0.223    c-0.063,0.037-0.129,0.079-0.191,0.117c0.027-0.074,0.062-0.15,0.084-0.223c-0.969,0.576-2.082,1.353-3.221,1.771    c-0.017-0.016-0.033-0.033-0.05-0.049c-0.036-0.036-0.071-0.073-0.108-0.108c-0.034-0.035-0.069-0.071-0.104-0.104    c-0.034-0.035-0.069-0.072-0.106-0.107c-0.035-0.035-0.069-0.071-0.104-0.105c-0.035-0.035-0.069-0.072-0.106-0.106    c-0.033-0.034-0.066-0.07-0.102-0.103c-0.038-0.037-0.074-0.076-0.113-0.113c-0.033-0.034-0.068-0.07-0.104-0.104    c-0.035-0.035-0.07-0.072-0.105-0.105c-0.035-0.036-0.072-0.073-0.107-0.108c-1.145-0.906-2.201-1.56-3.795-1.56    c-3.398,0-6,2.602-6,6c0,0.398,0.047,0.7,0.11,0.956l-0.11,0.044c-4.803,0-8.301-0.822-11.063-3.059    c-0.008-0.01-0.016-0.018-0.024-0.028c-0.001,0.002-0.002,0.004-0.003,0.006c-0.005-0.004-0.01-0.007-0.015-0.011    c-0.029-0.035-0.059-0.067-0.088-0.102c-0.002,0.003-0.004,0.007-0.006,0.01c-0.033-0.04-0.067-0.076-0.101-0.116    c-0.002,0.003-0.004,0.007-0.006,0.01c-0.033-0.04-0.067-0.076-0.101-0.116c-0.002,0.003-0.003,0.006-0.005,0.01    c-0.033-0.04-0.068-0.076-0.101-0.117c-0.002,0.003-0.003,0.007-0.005,0.01c-0.033-0.04-0.068-0.076-0.101-0.117    c-0.002,0.003-0.003,0.007-0.005,0.01c-0.033-0.04-0.068-0.075-0.101-0.116c-0.002,0.003-0.003,0.007-0.005,0.01    c-0.033-0.04-0.068-0.076-0.101-0.117c-0.002,0.003-0.003,0.006-0.005,0.01c-0.033-0.04-0.068-0.076-0.101-0.116    c-0.002,0.003-0.004,0.007-0.006,0.01c-0.033-0.04-0.067-0.076-0.101-0.116c-0.002,0.003-0.003,0.007-0.005,0.01    c-0.033-0.04-0.068-0.076-0.101-0.117c-0.002,0.003-0.003,0.007-0.005,0.01c-0.033-0.04-0.068-0.076-0.101-0.117    c-0.002,0.003-0.003,0.007-0.005,0.01c-0.033-0.04-0.068-0.076-0.101-0.117c-0.002,0.003-0.003,0.007-0.005,0.01    c-0.033-0.04-0.068-0.076-0.101-0.117c-0.002,0.003-0.003,0.007-0.005,0.01c-0.033-0.04-0.068-0.075-0.101-0.116    c-0.002,0.003-0.003,0.007-0.005,0.01c-0.033-0.04-0.068-0.076-0.101-0.116c-0.002,0.003-0.003,0.007-0.005,0.01    c-0.033-0.04-0.068-0.076-0.101-0.117c-0.002,0.003-0.003,0.007-0.005,0.01c-0.033-0.04-0.068-0.076-0.101-0.117    c-0.002,0.003-0.003,0.007-0.005,0.01c-0.033-0.04-0.068-0.076-0.101-0.117c-0.531,0.908-1,1.872-1,3    c0,1.625,0.783,3.03,1.885,4.097c0.035,0.035,0.069,0.07,0.104,0.104c0.036,0.037,0.072,0.073,0.109,0.109    c0.034,0.035,0.068,0.069,0.104,0.104c0.036,0.036,0.072,0.073,0.108,0.108c0.036,0.036,0.071,0.072,0.108,0.108    c0.034,0.034,0.068,0.068,0.103,0.103c0.036,0.036,0.071,0.072,0.107,0.107c0.036,0.036,0.07,0.071,0.106,0.106    c0.006,0.006,0.011,0.013,0.018,0.019c-0.95-0.09-1.991-0.538-2.754-0.959c0,1.341,0.85,2.775,2.039,4.001    c0.034,0.036,0.071,0.069,0.105,0.104c0.035,0.037,0.073,0.072,0.109,0.109c0.035,0.037,0.073,0.071,0.108,0.108    c0.033,0.034,0.07,0.067,0.104,0.103c0.034,0.036,0.071,0.07,0.105,0.105c0.035,0.037,0.074,0.072,0.109,0.109    c0.033,0.034,0.069,0.066,0.102,0.103c0.035,0.036,0.074,0.071,0.11,0.108c0.035,0.039,0.074,0.072,0.109,0.109    c0.031,0.033,0.065,0.063,0.097,0.098c0.037,0.037,0.076,0.074,0.113,0.112c0.035,0.037,0.073,0.072,0.108,0.108    c0.033,0.035,0.068,0.066,0.102,0.102c0.034,0.036,0.072,0.07,0.106,0.105c0.035,0.037,0.074,0.072,0.109,0.109    c0.032,0.034,0.066,0.065,0.099,0.1c0.037,0.037,0.076,0.074,0.112,0.113c0.035,0.037,0.073,0.07,0.108,0.107    c0.033,0.034,0.069,0.066,0.103,0.104c0.035,0.035,0.072,0.07,0.108,0.106c0.035,0.037,0.073,0.072,0.109,0.108    c0.032,0.035,0.068,0.066,0.101,0.102c0.035,0.037,0.073,0.072,0.108,0.107c0.034,0.037,0.072,0.07,0.107,0.106    c0.034,0.036,0.07,0.069,0.104,0.104c0.035,0.037,0.073,0.072,0.109,0.109c0.032,0.033,0.067,0.065,0.1,0.101    c0.036,0.038,0.075,0.073,0.112,0.112c0.034,0.035,0.071,0.068,0.105,0.104c0.034,0.035,0.071,0.069,0.105,0.104    c0.034,0.037,0.072,0.07,0.106,0.106c0.035,0.037,0.074,0.072,0.109,0.108c0.016,0.018,0.034,0.033,0.05,0.051    c-1.42,0.058-3.229-0.4-3.49-0.451c0.296,0.925,0.843,1.714,1.552,2.342c0.037,0.034,0.068,0.072,0.106,0.106    c0.037,0.034,0.069,0.073,0.107,0.106c0.037,0.034,0.069,0.073,0.106,0.106c0.038,0.034,0.07,0.073,0.108,0.106    c0.036,0.033,0.067,0.07,0.104,0.104s0.069,0.073,0.107,0.106c0.037,0.035,0.068,0.072,0.106,0.105    c0.037,0.035,0.068,0.073,0.106,0.105c0.037,0.035,0.068,0.072,0.105,0.105c0.037,0.034,0.069,0.073,0.106,0.107    c0.037,0.033,0.069,0.072,0.107,0.105c0.037,0.035,0.069,0.073,0.107,0.107c0.037,0.033,0.068,0.072,0.105,0.104    c0.037,0.034,0.068,0.072,0.105,0.105c0.037,0.034,0.07,0.073,0.108,0.106c0.036,0.034,0.066,0.071,0.103,0.104    c0.037,0.034,0.069,0.073,0.107,0.106c0.038,0.035,0.07,0.074,0.108,0.108c0.037,0.034,0.068,0.072,0.105,0.104    c0.037,0.035,0.069,0.074,0.107,0.107c0.037,0.033,0.068,0.072,0.105,0.104c0.037,0.035,0.069,0.074,0.107,0.107    c0.037,0.034,0.069,0.073,0.107,0.105c0.036,0.033,0.066,0.072,0.103,0.104c0.038,0.035,0.071,0.075,0.109,0.109    c0.035,0.032,0.064,0.069,0.1,0.101c-1.731,0.928-3.691,1.408-5.842,1.408c-0.498,0-0.987-0.029-1.469-0.084    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.088-0.006c0.062,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.003-0.089-0.005    c0.062,0.039,0.132,0.072,0.195,0.111c-0.029-0.004-0.059-0.003-0.088-0.006c0.062,0.039,0.13,0.072,0.193,0.11    c-0.029-0.003-0.059-0.002-0.088-0.005c0.062,0.039,0.132,0.072,0.195,0.111c-0.029-0.004-0.059-0.002-0.088-0.006    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.071,0.195,0.11    c-0.029-0.002-0.06-0.002-0.088-0.004c0.062,0.039,0.132,0.071,0.195,0.11c-0.029-0.003-0.06-0.002-0.089-0.005    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.059-0.002-0.088-0.006c0.062,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.039,0.13,0.071,0.193,0.11c-0.028-0.004-0.058-0.002-0.086-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.072,0.195,0.11    c-0.029-0.002-0.059-0.002-0.088-0.004c0.062,0.039,0.132,0.072,0.195,0.11c-0.029-0.003-0.06-0.002-0.089-0.005    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.089-0.006c0.062,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005    c0.062,0.039,0.13,0.072,0.193,0.109c-0.029-0.002-0.059-0.002-0.087-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.089-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.071,0.195,0.11    c-0.029-0.002-0.06-0.002-0.088-0.004c0.062,0.039,0.132,0.071,0.195,0.11c-0.029-0.003-0.06-0.002-0.089-0.005    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.088-0.006c0.062,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.039,0.13,0.072,0.193,0.11c-0.028-0.004-0.058-0.002-0.086-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.039,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.088-0.006    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.071,0.195,0.11    c-0.029-0.002-0.06-0.002-0.089-0.004c0.062,0.039,0.132,0.071,0.195,0.11c-0.029-0.003-0.06-0.002-0.088-0.005    c0.062,0.039,0.13,0.072,0.193,0.109c-0.028-0.002-0.058-0.002-0.086-0.004c0.062,0.039,0.132,0.071,0.195,0.11    c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.088-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.071,0.195,0.11    c-0.029-0.002-0.06-0.002-0.088-0.004c0.062,0.039,0.132,0.071,0.195,0.11c-0.029-0.003-0.06-0.002-0.088-0.005    c0.062,0.039,0.13,0.072,0.193,0.109c-0.028-0.002-0.059-0.002-0.087-0.004c0.063,0.039,0.133,0.072,0.197,0.111    c-0.03-0.003-0.061-0.002-0.09-0.005c0.062,0.039,0.13,0.072,0.193,0.11c-0.029-0.004-0.059-0.002-0.087-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.002-0.059-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.039,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.088-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.071,0.195,0.11    c-0.029-0.002-0.059-0.002-0.088-0.004c0.062,0.039,0.132,0.071,0.195,0.11c-0.029-0.003-0.06-0.002-0.089-0.005    c0.062,0.039,0.13,0.072,0.193,0.109c-0.028-0.002-0.058-0.002-0.086-0.004c0.062,0.039,0.132,0.071,0.195,0.11    c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.059-0.002-0.088-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.003-0.06-0.003-0.088-0.005c0.062,0.039,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.003-0.088-0.006    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.006c0.062,0.039,0.13,0.072,0.193,0.111    c-0.028-0.004-0.059-0.002-0.087-0.006c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.059-0.002-0.088-0.005    c0.062,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.06-0.002-0.088-0.004c0.062,0.039,0.132,0.071,0.195,0.11    c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.088-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.071,0.195,0.11    c-0.029-0.002-0.059-0.002-0.088-0.004c0.062,0.039,0.132,0.071,0.195,0.11c-0.029-0.003-0.06-0.002-0.089-0.005    c0.062,0.039,0.13,0.072,0.193,0.109c-0.028-0.002-0.059-0.002-0.087-0.004c0.062,0.039,0.132,0.072,0.195,0.11    c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.088-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.039,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.089-0.006    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.071,0.195,0.11    c-0.029-0.002-0.06-0.002-0.088-0.004c0.062,0.039,0.13,0.071,0.193,0.109c-0.028-0.003-0.059-0.002-0.087-0.005    c0.062,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.06-0.002-0.088-0.004c0.062,0.039,0.132,0.071,0.195,0.11    c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.088-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.11    c-0.029-0.002-0.06-0.002-0.088-0.004c0.062,0.039,0.131,0.072,0.193,0.109c-0.029-0.003-0.059-0.002-0.088-0.005    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.059-0.003-0.088-0.005c0.062,0.039,0.132,0.072,0.195,0.111    c-0.029-0.004-0.06-0.002-0.088-0.006c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005    c0.062,0.038,0.13,0.071,0.193,0.11c-0.028-0.004-0.058-0.002-0.086-0.006c0.063,0.04,0.133,0.073,0.197,0.112    c-0.03-0.003-0.061-0.002-0.091-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.059-0.002-0.088-0.006    c0.062,0.041,0.132,0.073,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.039,0.13,0.071,0.193,0.11    c-0.029-0.004-0.059-0.002-0.087-0.006c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005    c0.062,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.059-0.002-0.088-0.004c0.062,0.039,0.132,0.071,0.195,0.11    c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.088-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.003-0.059-0.002-0.088-0.005c0.062,0.039,0.13,0.072,0.193,0.109c-0.028-0.003-0.059-0.002-0.087-0.005    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.039,0.132,0.072,0.195,0.111    c-0.029-0.004-0.06-0.002-0.088-0.006c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005    c0.062,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.06-0.002-0.088-0.004c0.062,0.039,0.13,0.071,0.193,0.109    c-0.029-0.003-0.059-0.002-0.088-0.005c0.063,0.04,0.133,0.073,0.197,0.112c-0.029-0.004-0.06-0.002-0.089-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.072,0.195,0.11    c-0.029-0.002-0.06-0.002-0.088-0.004c0.062,0.039,0.13,0.072,0.193,0.109c-0.029-0.003-0.059-0.002-0.087-0.005    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.003-0.088-0.005c0.062,0.039,0.132,0.072,0.195,0.111    c-0.029-0.004-0.06-0.003-0.088-0.006c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.006    c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.002-0.06-0.002-0.089-0.005c0.062,0.039,0.131,0.072,0.193,0.11    c-0.029-0.003-0.059-0.002-0.087-0.005c0.063,0.04,0.133,0.073,0.197,0.112c-0.029-0.004-0.06-0.002-0.089-0.006    c0.062,0.039,0.13,0.072,0.193,0.11c-0.029-0.003-0.059-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.004-0.06-0.002-0.088-0.006c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005    c0.062,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.06-0.002-0.088-0.004c0.062,0.039,0.132,0.071,0.195,0.11    c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.039,0.13,0.072,0.193,0.109c-0.028-0.002-0.058-0.002-0.086-0.004    c0.063,0.039,0.133,0.072,0.197,0.111c-0.03-0.003-0.061-0.002-0.091-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.003-0.059-0.003-0.087-0.005c0.062,0.039,0.131,0.072,0.193,0.109c-0.029-0.003-0.059-0.002-0.088-0.005    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.039,0.132,0.072,0.195,0.111    c-0.029-0.004-0.06-0.002-0.088-0.006c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005    c0.062,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.06-0.002-0.089-0.004c0.062,0.039,0.13,0.071,0.193,0.109    c-0.029-0.003-0.059-0.002-0.087-0.005c0.062,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.059-0.002-0.088-0.004    c0.062,0.039,0.132,0.071,0.195,0.11c-0.029-0.003-0.06-0.002-0.089-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.004-0.06-0.002-0.088-0.006c0.062,0.039,0.13,0.072,0.193,0.11c-0.028-0.003-0.058-0.002-0.087-0.005    c0.063,0.04,0.133,0.073,0.197,0.111c-0.03-0.002-0.061-0.002-0.09-0.004c0.062,0.039,0.132,0.072,0.195,0.11    c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.088-0.006    c0.062,0.039,0.13,0.072,0.193,0.111c-0.029-0.004-0.059-0.003-0.088-0.006c0.063,0.04,0.133,0.073,0.197,0.112    c-0.029-0.003-0.061-0.002-0.09-0.005c0.062,0.039,0.13,0.072,0.193,0.109c-0.029-0.002-0.059-0.002-0.087-0.005    c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.004-0.06-0.002-0.089-0.006c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.088-0.005    c0.062,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.059-0.002-0.088-0.004c0.062,0.039,0.13,0.071,0.193,0.109    c-0.029-0.003-0.059-0.002-0.088-0.005c0.062,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.06-0.002-0.088-0.004    c0.062,0.039,0.132,0.071,0.195,0.11c-0.029-0.003-0.06-0.002-0.088-0.005c0.062,0.04,0.132,0.072,0.195,0.111    c-0.029-0.004-0.059-0.002-0.088-0.006c0.062,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005    c0.062,0.039,0.13,0.072,0.193,0.11c-0.028-0.004-0.058-0.002-0.086-0.006c0.063,0.041,0.133,0.074,0.197,0.113    c-0.03-0.004-0.061-0.002-0.091-0.006c0.062,0.04,0.132,0.072,0.195,0.111c-0.029-0.003-0.059-0.002-0.088-0.005    c0.062,0.04,0.132,0.073,0.195,0.11c-0.03-0.002-0.061-0.002-0.09-0.004c0.062,0.038,0.131,0.071,0.193,0.109    c-0.029-0.003-0.06-0.002-0.088-0.005c0.063,0.04,0.133,0.071,0.195,0.11c-0.029-0.002-0.061-0.002-0.089-0.004    c0.063,0.039,0.132,0.071,0.194,0.11c-0.028-0.003-0.06-0.002-0.088-0.005c0.063,0.04,0.131,0.072,0.194,0.111    c-0.028-0.004-0.06-0.002-0.089-0.006c0.063,0.041,0.132,0.072,0.195,0.111c-0.03-0.003-0.062-0.002-0.09-0.005    c0.062,0.039,0.131,0.071,0.192,0.11c-0.028-0.004-0.06-0.002-0.087-0.006c0.063,0.041,0.133,0.073,0.197,0.112    c-0.03-0.003-0.063-0.002-0.092-0.005c0.063,0.039,0.131,0.072,0.193,0.109c-0.027-0.002-0.059-0.002-0.087-0.004    c0.063,0.039,0.132,0.072,0.195,0.11c-0.029-0.003-0.06-0.002-0.089-0.005c0.063,0.04,0.132,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.09-0.006c0.063,0.039,0.131,0.072,0.193,0.111c-0.028-0.004-0.059-0.002-0.088-0.006    c0.063,0.04,0.134,0.073,0.197,0.112c-0.029-0.003-0.062-0.002-0.091-0.005c0.062,0.039,0.132,0.072,0.193,0.109    c-0.029-0.002-0.06-0.002-0.088-0.004c0.063,0.039,0.133,0.072,0.196,0.111c-0.029-0.003-0.062-0.002-0.09-0.005    c0.062,0.039,0.131,0.071,0.192,0.11c-0.028-0.004-0.06-0.002-0.087-0.006c0.063,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.071,0.195,0.11c-0.029-0.002-0.061-0.002-0.089-0.004    c0.063,0.039,0.132,0.071,0.194,0.11c-0.028-0.003-0.06-0.002-0.088-0.005c0.063,0.04,0.131,0.072,0.194,0.111    c-0.028-0.004-0.06-0.002-0.089-0.006c0.063,0.039,0.131,0.072,0.193,0.11c-0.028-0.003-0.06-0.002-0.088-0.005    c0.063,0.04,0.134,0.073,0.197,0.112c-0.029-0.003-0.062-0.001-0.092-0.005c0.063,0.039,0.131,0.072,0.193,0.109    c-0.028-0.003-0.059-0.002-0.088-0.005c0.063,0.04,0.133,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005    c0.063,0.039,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.089-0.006c0.063,0.04,0.132,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.071,0.195,0.11c-0.029-0.002-0.061-0.002-0.089-0.004    c0.062,0.038,0.131,0.071,0.192,0.109c-0.029-0.003-0.059-0.002-0.086-0.005c0.063,0.04,0.133,0.073,0.195,0.112    c-0.029-0.004-0.061-0.002-0.09-0.006c0.063,0.039,0.131,0.072,0.193,0.11c-0.029-0.003-0.061-0.002-0.088-0.005    c0.063,0.04,0.131,0.072,0.194,0.111c-0.028-0.004-0.06-0.002-0.088-0.006c0.063,0.041,0.132,0.073,0.194,0.111    c-0.028-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.072,0.195,0.11c-0.029-0.002-0.061-0.002-0.089-0.004    c0.063,0.039,0.132,0.072,0.194,0.11c-0.028-0.003-0.059-0.002-0.088-0.005c0.062,0.039,0.131,0.072,0.192,0.11    c-0.028-0.003-0.059-0.003-0.087-0.005c0.063,0.039,0.133,0.072,0.197,0.111c-0.03-0.003-0.063-0.002-0.092-0.005    c0.063,0.039,0.132,0.072,0.193,0.11c-0.029-0.003-0.06-0.002-0.088-0.006c0.063,0.041,0.133,0.074,0.197,0.113    c-0.03-0.004-0.063-0.002-0.092-0.006c0.063,0.039,0.132,0.072,0.193,0.11c-0.027-0.003-0.059-0.002-0.086-0.005    c0.063,0.04,0.131,0.072,0.194,0.111c-0.029-0.004-0.06-0.002-0.089-0.006c0.063,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.039,0.131,0.071,0.193,0.11c-0.029-0.004-0.059-0.002-0.088-0.006    c0.063,0.041,0.133,0.073,0.197,0.112c-0.029-0.003-0.062-0.002-0.09-0.005c0.062,0.039,0.131,0.072,0.192,0.109    c-0.028-0.002-0.06-0.002-0.088-0.004c0.063,0.039,0.133,0.072,0.196,0.111c-0.029-0.003-0.062-0.002-0.09-0.005    c0.062,0.039,0.131,0.072,0.192,0.11c-0.028-0.004-0.06-0.002-0.087-0.006c0.063,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.072,0.195,0.111c-0.029-0.003-0.06-0.003-0.089-0.005    c0.063,0.039,0.132,0.072,0.195,0.111c-0.03-0.004-0.062-0.002-0.091-0.006c0.063,0.04,0.133,0.072,0.195,0.111    c-0.029-0.003-0.06-0.002-0.088-0.005c0.063,0.038,0.131,0.071,0.193,0.11c-0.029-0.004-0.061-0.002-0.088-0.006    c0.063,0.04,0.133,0.073,0.197,0.112c-0.03-0.003-0.063-0.002-0.092-0.005c0.063,0.039,0.131,0.072,0.193,0.109    c-0.028-0.002-0.059-0.002-0.087-0.004c0.063,0.039,0.132,0.071,0.194,0.11c-0.028-0.003-0.06-0.002-0.088-0.005    c0.063,0.04,0.131,0.072,0.194,0.111c-0.028-0.004-0.06-0.002-0.089-0.006c0.063,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.071,0.195,0.11c-0.029-0.002-0.061-0.002-0.089-0.004    c0.062,0.039,0.131,0.071,0.192,0.109c-0.027-0.003-0.059-0.002-0.086-0.005c0.063,0.04,0.133,0.073,0.197,0.112    c-0.031-0.004-0.063-0.002-0.092-0.006c0.063,0.039,0.131,0.072,0.193,0.11c-0.029-0.003-0.059-0.002-0.088-0.005    c0.063,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.061-0.002-0.09-0.006c0.063,0.041,0.133,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.072,0.195,0.111c-0.029-0.003-0.06-0.002-0.089-0.005    c0.063,0.039,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.089-0.006c0.063,0.039,0.131,0.072,0.193,0.11    c-0.029-0.003-0.06-0.002-0.088-0.005c0.063,0.04,0.133,0.072,0.196,0.111c-0.028-0.002-0.062-0.002-0.09-0.004    c0.062,0.039,0.131,0.071,0.192,0.109c-0.028-0.003-0.06-0.002-0.088-0.005c0.063,0.04,0.133,0.073,0.196,0.112    c-0.028-0.004-0.062-0.002-0.09-0.006c0.062,0.039,0.131,0.072,0.192,0.11c-0.027-0.003-0.059-0.002-0.086-0.005    c0.063,0.04,0.131,0.072,0.194,0.111c-0.028-0.004-0.06-0.002-0.089-0.006c0.063,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.072,0.195,0.111c-0.029-0.003-0.061-0.003-0.09-0.005    c0.063,0.039,0.132,0.072,0.195,0.111c-0.029-0.004-0.061-0.003-0.088-0.006c0.062,0.039,0.131,0.072,0.193,0.11    c-0.029-0.003-0.061-0.002-0.089-0.005c0.063,0.039,0.134,0.072,0.196,0.111c-0.029-0.002-0.061-0.002-0.09-0.004    c0.062,0.038,0.131,0.071,0.193,0.109c-0.029-0.003-0.06-0.002-0.088-0.005c0.063,0.04,0.133,0.071,0.195,0.11    c-0.029-0.002-0.061-0.002-0.089-0.004c0.063,0.039,0.132,0.071,0.194,0.11c-0.028-0.003-0.06-0.002-0.088-0.005    c0.063,0.04,0.131,0.072,0.194,0.111c-0.03-0.004-0.062-0.002-0.09-0.006c0.063,0.041,0.132,0.072,0.194,0.111    c-0.028-0.003-0.06-0.002-0.088-0.005c0.063,0.039,0.131,0.071,0.193,0.11c-0.029-0.004-0.059-0.002-0.088-0.006    c0.063,0.041,0.133,0.074,0.195,0.112c-0.028-0.003-0.061-0.002-0.09-0.005c0.063,0.039,0.131,0.072,0.193,0.11    c-0.027-0.003-0.059-0.003-0.087-0.005c0.063,0.039,0.132,0.072,0.194,0.111c-0.028-0.004-0.06-0.003-0.088-0.006    c0.063,0.04,0.131,0.072,0.194,0.111c-0.028-0.003-0.06-0.002-0.089-0.006c0.063,0.041,0.133,0.072,0.195,0.111    c-0.029-0.002-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.073,0.195,0.111c-0.029-0.003-0.061-0.002-0.09-0.005    c0.063,0.04,0.133,0.072,0.194,0.111c-0.028-0.004-0.06-0.002-0.087-0.006c0.063,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.062-0.002-0.091-0.005c0.063,0.039,0.132,0.071,0.193,0.11c-0.028-0.004-0.06-0.002-0.087-0.006    c0.063,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.071,0.195,0.11    c-0.029-0.002-0.061-0.002-0.089-0.004c0.063,0.039,0.132,0.071,0.194,0.11c-0.028-0.003-0.06-0.002-0.088-0.005    c0.063,0.04,0.131,0.072,0.194,0.111c-0.028-0.004-0.06-0.002-0.089-0.006c0.063,0.041,0.132,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.073,0.195,0.111c-0.029-0.003-0.061-0.002-0.09-0.005    c0.063,0.039,0.131,0.072,0.193,0.109c-0.028-0.002-0.06-0.002-0.088-0.005c0.063,0.04,0.133,0.072,0.195,0.111    c-0.029-0.003-0.06-0.002-0.089-0.005c0.063,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.089-0.006    c0.063,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.071,0.195,0.11    c-0.029-0.002-0.061-0.002-0.089-0.004c0.063,0.039,0.132,0.071,0.194,0.11c-0.029-0.003-0.061-0.002-0.09-0.005    c0.063,0.04,0.133,0.072,0.195,0.111c-0.029-0.004-0.061-0.002-0.088-0.006c0.063,0.039,0.131,0.072,0.193,0.11    c-0.029-0.003-0.061-0.002-0.088-0.005c0.063,0.04,0.131,0.072,0.194,0.111c-0.028-0.004-0.06-0.002-0.089-0.006    c0.063,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.089-0.005c0.063,0.039,0.132,0.072,0.194,0.111c-0.028-0.004-0.06-0.002-0.088-0.006    c0.063,0.04,0.131,0.072,0.194,0.111c-0.03-0.003-0.062-0.002-0.09-0.005c0.063,0.04,0.132,0.071,0.194,0.11    c-0.028-0.002-0.059-0.002-0.088-0.004c0.063,0.038,0.132,0.071,0.193,0.109c-0.029-0.003-0.06-0.002-0.088-0.005    c0.063,0.04,0.133,0.071,0.195,0.11c-0.029-0.002-0.061-0.002-0.09-0.004c0.063,0.039,0.133,0.071,0.195,0.11    c-0.029-0.003-0.06-0.002-0.089-0.005c0.063,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.089-0.006    c0.063,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.072,0.195,0.11    c-0.029-0.002-0.061-0.002-0.09-0.004c0.063,0.039,0.132,0.072,0.195,0.11c-0.029-0.003-0.061-0.002-0.09-0.005    c0.063,0.039,0.131,0.072,0.193,0.11c-0.027-0.003-0.059-0.003-0.087-0.005c0.063,0.039,0.132,0.072,0.194,0.111    c-0.028-0.004-0.06-0.003-0.088-0.006c0.063,0.04,0.131,0.072,0.194,0.111c-0.028-0.003-0.06-0.002-0.089-0.005    c0.063,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.061-0.002-0.09-0.004c0.063,0.039,0.133,0.071,0.195,0.11    c-0.029-0.003-0.06-0.002-0.089-0.005c0.063,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.089-0.006    c0.063,0.041,0.132,0.073,0.195,0.111c-0.029-0.003-0.062-0.002-0.091-0.005c0.062,0.04,0.132,0.071,0.194,0.11    c-0.028-0.002-0.059-0.002-0.088-0.004c0.063,0.039,0.132,0.071,0.193,0.109c-0.029-0.003-0.06-0.002-0.088-0.005    c0.063,0.04,0.133,0.071,0.195,0.11c-0.029-0.002-0.061-0.002-0.089-0.004c0.063,0.039,0.132,0.071,0.194,0.11    c-0.028-0.003-0.06-0.002-0.088-0.005c0.063,0.04,0.131,0.072,0.194,0.111c-0.028-0.004-0.06-0.002-0.089-0.006    c0.063,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.04,0.133,0.072,0.195,0.111    c-0.029-0.003-0.061-0.002-0.089-0.005c0.063,0.039,0.132,0.072,0.194,0.111c-0.028-0.004-0.061-0.002-0.09-0.006    c0.063,0.039,0.131,0.072,0.193,0.11c-0.027-0.003-0.059-0.002-0.087-0.005c0.062,0.04,0.132,0.072,0.194,0.111    c-0.028-0.004-0.06-0.002-0.088-0.006c0.063,0.041,0.131,0.072,0.194,0.111c-0.028-0.003-0.06-0.002-0.089-0.005    c0.063,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.061-0.002-0.09-0.004c0.063,0.039,0.133,0.071,0.195,0.11    c-0.029-0.003-0.06-0.002-0.089-0.005c0.063,0.04,0.132,0.072,0.195,0.111c-0.029-0.004-0.06-0.002-0.089-0.006    c0.063,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.062-0.002-0.091-0.005c0.062,0.039,0.132,0.072,0.193,0.11    c-0.028-0.004-0.06-0.002-0.087-0.006c0.063,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.061-0.002-0.09-0.005    c0.063,0.04,0.133,0.072,0.195,0.111c-0.029-0.003-0.061-0.002-0.089-0.005c0.063,0.039,0.132,0.072,0.194,0.111    c-0.028-0.004-0.06-0.002-0.088-0.006c0.063,0.04,0.131,0.072,0.194,0.111c-0.028-0.003-0.06-0.002-0.089-0.005    c0.063,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.061-0.002-0.09-0.004c0.063,0.039,0.133,0.071,0.195,0.11    c-0.029-0.003-0.061-0.002-0.09-0.005c0.063,0.039,0.131,0.072,0.193,0.109c-0.028-0.002-0.06-0.002-0.088-0.004    c0.063,0.039,0.133,0.071,0.194,0.11c-0.028-0.003-0.059-0.002-0.088-0.005c0.063,0.04,0.132,0.072,0.195,0.111    c-0.029-0.004-0.06-0.002-0.089-0.006c0.063,0.041,0.132,0.072,0.195,0.111c-0.029-0.003-0.061-0.002-0.09-0.005    c0.063,0.04,0.133,0.072,0.195,0.11c-0.029-0.002-0.061-0.002-0.09-0.004c0.063,0.039,0.133,0.072,0.195,0.11    c-0.029-0.003-0.06-0.002-0.088-0.005c0.063,0.04,0.131,0.072,0.194,0.111c-0.029-0.003-0.06-0.002-0.089-0.006    c0.063,0.039,0.131,0.072,0.193,0.111c-0.029-0.004-0.061-0.002-0.088-0.006c0.063,0.04,0.131,0.072,0.194,0.111    c-0.028-0.003-0.06-0.002-0.089-0.005c0.063,0.04,0.132,0.071,0.195,0.11c-0.029-0.002-0.061-0.002-0.09-0.004    c0.063,0.039,0.133,0.071,0.195,0.11c-0.029-0.003-0.061-0.002-0.089-0.005c2.724,1.744,6.522,2.574,10,2.574    c11.321,0,17-8.867,17-17c0-0.269,0.008-0.736,0-1C77.172,57.632,78.143,56.441,78.971,55.209z" enable-background="new    " fill="url(#SVGID_3_)" opacity="0.3"/></g></g><path d="M47,23.209c-1.105,0.49-2.756,1.142-4,1.291c1.273-0.762,2.539-2.561,3-4  c-0.971,0.577-2.087,1.355-3.227,1.773L42,21.5c-1.121-1.197-2.23-2-4-2c-3.398,0-6,2.602-6,6c0,0.398,0.047,0.7,0.11,0.956L32,26.5  c-6,0-9.966-1.276-13-5c-0.531,0.908-1,1.872-1,3c0,2.136,1.348,3.894,3,5c-1.009-0.033-2.171-0.542-3-1c0,2.98,4.186,6.432,7,7  c-1,1-4.623,0.074-5,0c0.784,2.447,3.309,3.949,6,4c-2.105,1.648-4.647,2.51-7.531,2.51c-0.498,0-0.987-0.029-1.469-0.084  c2.723,1.744,6.523,2.574,10,2.574c11.322,0,17-8.867,17-17c0-0.268,0.008-0.736,0-1C45.201,25.632,46.172,24.441,47,23.209z" fill="#FFFFFF" id="Brid_1_"/>
+  </svg>
+  </a>
+      </div>
+      <div class="jumbotron text-center">
+       <div class="content">
+         <span class="cellfiefuge">
+           Cellfiefuge
+         </span>
+         <span class="tagline">
+           Project description
+         </span>
+       </div>
      </div>
-     <div >
+     <div class="container-fluid">
-       <div class="col-sm-4"> Hello bootstrap.
+       <div class="row">
+        <div id="carousel-example-generic" class="carousel slide" data-ride="carousel">
+    <!-- Indicators -->
+    <ol class="carousel-indicators">
+      <li data-target="#carousel-example-generic" data-slide-to="0" class="active"></li>
+      <li data-target="#carousel-example-generic" data-slide-to="1"></li>
+      <li data-target="#carousel-example-generic" data-slide-to="2"></li>
+    </ol>
+    <!-- Wrapper for slides -->
+    <div class="carousel-inner" role="listbox">
+      <div class="item active">
+        <img src="" alt="..." >
+        <div class="carousel-caption">
+          ...
+        </div>
        </div>
-       <div class="col-sm-8"> Hello bootstrap.
+       <div class="item">
+        <img src="..." alt="...">
+        <div class="carousel-caption">
+          ...
+        </div>
        </div>
+      ...
+    </div>
+    <!-- Controls -->
+    <a class="left carousel-control" href="#carousel-example-generic" role="button" data-slide="prev">
+      <span class="glyphicon glyphicon-chevron-left" aria-hidden="true"></span>
+      <span class="sr-only">Previous</span>
+    </a>
+    <a class="right carousel-control" href="#carousel-example-generic" role="button" data-slide="next">
+      <span class="glyphicon glyphicon-chevron-right" aria-hidden="true"></span>
+      <span class="sr-only">Next</span>
+    </a>
+  </div>
+    </div>
+    <div class="row">
+    <div class="wrap col-md-12 ">
+      <svg class="descSvg" height="60" width="320" xmlns="http://www.w3.org/2000/svg">
+        <rect class="shape" height="60" width="320" />
+        <div class="calltoaction">
+          <a href="#overview">
+          <span class="arrowDown">&#8595;</span></a>
+        </div>
+      </svg>
+    </div>
+  </div>
+  </div>
+  <!--next page  -->
+  <div class="container-fluid" id="overview" >
+  <div class="row">
+    <div class="toCompensate col-md-12"> </div>
+  </div>
+  <div class="row">
+    <div class="col-md-6 box">
+      <div class="innerBox" onclick="openOverview()">Overview</div>
+    </div>
+    <div class="col-md-6 box">
+      <div class="innerBox" onclick="openDesign()">Design</div>
+    </div>
+  </div>
+  <div class="row">
+    <div class="col-md-6 box">
+      <div class="innerBox" onclick="openProtein()">Protein production module</div>
+    </div>
+    <div class="col-md-6 box">
+      <div class="innerBox" onclick="openAggr()">Auto aggregation module</div>
+    </div>
+  </div>
+  </div>
+  <!-- 2nd page -->
+  <div id="overviewTab" class="overviewTab">
+    <a href="javascript:void(0)" class="closebtn" onclick="closeBar()">&times;</a>
+    <div class="row">
+      <div class="col-md-12 overviewTabcontent">
+        <h1>Overview</h1>
+        <p>Since the advent of rDNA technology in the late 1970s, the use of recombinant protein has been on the rise, starting from insulin and hormones all the way through to monoclonal antibodies and one hundred and fifty-one FDA approved protein based recombinant pharmaceuticals by the year 2009<a href="#bull1" onclick="closeBar()">[1]</a>. Despite being in high demand, recombinant proteins have heavy price tags, thanks to the significant manufacturing costs. One of the important steps in the manufacturing process is Centrifugation but on an industrial scale, it is an economic nightmare. <a href="#bull2" onclick="closeBar()">[2]</a> Further, existing industrial setups use complex sensors to monitor cell density to determine the point at which to begin separation of the cells from the cell culture. One can immediately see the humongous relief that would be associated with finding a way to deal with the above problems. Hence, we came up with the idea of our project, the Cellfifuge.
+        </p>
+      </div>
+    </div>
+  </div>
+  <div id="designTab" class="designTab">
+    <a href="javascript:void(0)" class="closebtn" onclick="closeBar()">&times;</a>
+    <div class="row">
+      <div class="col-md-6 designTabcontent">
+        <h1>Our Bioreactor Design</h1>
+        <p>We realized that manufacturing units use expensive and energy expensive machinery <a href="#bull3" onclick="closeBar()">[3]</a>. Hence, we engineered our bacteria in such a way that it could do most of the work done by these monstrous machines so that we no longer have to buy them and pay their bills. To increase the yield of the product, we came up with the idea of separating cell growth and target protein production so that when the cells have grown up to a high enough cell density, they shuttle all their energy towards the required protein production.<a href="#bull4" onclick="closeBar()">[4]</a> Based on the above ideas, we came up with the design of our bioreactor. (Figure 1)
+We realized that we would not be able to work on the whole idea this summer so, we focussed on two modules of our design: the Protein production module and the auto-aggregation module leaving the arresting of cell growth for future iGEM teams as a seed for their ideas.
+        </p>
+      </div>
+      <div class="col-md-6 designTabcontent1">
+        <div class="bioreactorImgwrap">
+        <img class="bioreactorImg" src="file:///media/jessica/4C84DF1584DF007E/iGem/site/14Bootstrap/designa.jpg" />
+      </div>
+    </div>
+    </div>
+  </div>
+  <div id="proteinTab" class="proteinTab">
+    <a href="javascript:void(0)" class="closebtn" onclick="closeBar()">&times;</a>
+    <div class="row">
+      <div class="col-md-6 proteinTabcontent">
+        <h1>Protein Production Module</h1>
+        <p>Induction synthesis of protein of interest is another important step. Microorganisms in bioreactors are induced at the OD, at which the protein production is maximum. <a href="#bull5" onclick="closeBar()">[5]</a> Because of this, factories install sophisticated machinery to keep track of the OD value of the culture so as to know when to induce the production. We wanted our bacteria to know when to start protein production and start it by itself. Hence, we used the lux quorum sensing system to sense this cell density. <a href="#bull6" onclick="closeBar()">[6]</a> We have expressed the gene for our protein of interest under the luxI gene so that protein of interest is produced in significant quantities only when the quorum is achieved.(Figure 2) We also wish to tune the quorum sensing system by tweaking with the strength of the promoters involved in quorum sensing.
+        </p>
+      </div>
+      <div class="col-md-6 proteinTabcontent">
+        <div class="bioreactorImgwrap">
+        <img class="quorumImg" src="file:///media/jessica/4C84DF1584DF007E/iGem/site/14Bootstrap/lux.jpg" />
+      </div>
+    </div>
+    </div>
+  </div>
+  <div id="aggrTab" class="aggrTab">
+    <a href="javascript:void(0)" class="closebtn" onclick="closeBar()">&times;</a>
+    <div class="row">
+      <div class="col-md-6 aggrTabcontent">
+        <h1>Protein Production Module</h1>
+        <p>Induction synthesis of protein of interest is another important step. Microorganisms in bioreactors are induced at the OD, at which the protein production is maximum. <a href="#bull6" onclick="closeBar()">[6]</a> Because of this, factories install sophisticated machinery to keep track of the OD value of the culture so as to know when to induce the production. We wanted our bacteria to know when to start protein production and start it by itself. Hence, we used the lux quorum sensing system to sense this cell density. <a href="#bull7" onclick="closeBar()">[7]</a> We have expressed the gene for our protein of interest under the luxI gene so that protein of interest is produced in significant quantities only when the quorum is achieved.(Figure 2) We also wish to tune the quorum sensing system by tweaking with the strength of the promoters involved in quorum sensing.
+        </p>
+      </div>
+      <div class="col-md-6 aggrTabcontent">
+        <div class="bioreactorImgwrap">
+        <img class="aggrImg" src="file:///media/jessica/4C84DF1584DF007E/iGem/site/14Bootstrap/tube.jpg" />
+      </div>
+    </div>
+    </div>
+  </div>
+  <div class="row">
+    <div class="col-md-6 references" id="ref">
+      <h1>References</h1>
+      <ul>
+        <div class="ref">
+        <li><span id="bull1" class="label label-primary">[1]</span>Ferrer-Miralles N, Domingo-Espin J, Corchero JL, Vazquez E, Villaverde A. Microbial factories for recombinant pharmaceuticals, Microbial Cell Factories, 2009:8:17.</li>
+      </div>
+      <div>
+        <li ><span id="bull2" class="label label-primary">[2]</span>Medipally S.R., Yusoff F. Md., Banerjee S., Shariff M. Microalgae as sustainable renewable energy Feedstock for Biofuel Production, BioMed Research International, 2015.</li>
+      </div>
+      <div class="ref">
+        <li ><span id="bull3" class="label label-primary">[3]</span>Felo M., Christensen B., Higgins J., Process cost and facility considerations in the selection of primary cell culture clarification technology, AIChE, 2013.
+</li>
+        </div>
+        <div class="ref">
+        <li ><span id="bull4" class="label label-primary">[4]</span>Motoo Suzuki, Rohini Roy, Haiyan Zheng, Nancy Woychik, Masayori Inouye, Bacterial Bioreactors for High Yield Production of Recombinant Protein. The Journal of Biological Chemistry, 2006.</li>
+      </div>
+      <div class="ref">
+        <li ><span id="bull5" class="label label-primary">[5]</span>Rosano G.L., Ceccarelli E. A., Recombinant protein expression in Escherichia coli: advances and challenges, Frontiers in Microbiology, 2014.</li>
+      </div>
+      <div class="ref">
+        <li ><span id="bull6" class="label label-primary">[6]</span>Miller M.B., Bassler B.L., Quorum Sensing in Bacteria, Annual Review of Microbiology, 2001.</li>
+      </div>
+      <div class="ref">
+        <li ><span id="bull7" class="label label-primary">[7]</span>Marjan W. van der Woude, Ian R. Henderson, Regulation and Function of Ag43 (Flu), Annu. Rev. Microbiol., 2008.</li>
+      </div>
+      <!-- <span class="col-md-6 glyphicon glyphicon-list-alt"></span> -->
+      </ul>
+    </div>
+    <div class="col-md-6">
+      <!-- <span class="glyphicon glyphicon-list-alt"></span> -->
+    <!-- <i class="fa fa-list-ol fa-10x" aria-hidden="true"></i> -->
      </div>
-   </div>
+  </div>
    </body>
 </html>

Difference between revisions of "Team:IISc Bangalore/Description"

Revision as of 06:40, 25 August 2016

Overview

Our Bioreactor Design

Protein Production Module

Protein Production Module

References