Revision as of 13:56, 17 August 2016

Wednesday 17^th August

By Charlène

Clones 2 and 3 of GFP1-9_PSB1C3, and pPS16003, pPS16004 were extracted with the Plasmid MiniPrep kit.

The extracts were put to migrated on a 0.8%agarose gel with BET.

By Terrence

The purification were carried out following the usual protocol.

By Léa and Naiane

The cloning was carried out using a new protocol which uses pJET as cloning vector.

A heat shock transformation was made on the cloning samples using the following protocol

Revision as of 13:56, 17 August 2016 (view source) MahnaziGEM (Talk \| contribs) (→‎Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM) ← Older edit		Revision as of 13:56, 17 August 2016 (view source) MahnaziGEM (Talk \| contribs) (→‎Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM) Newer edit →
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	The cloning was carried out using a new [[Team:Paris_Saclay/Experiments#ligation with pJet\|protocol]] which uses pJET as cloning vector.		The cloning was carried out using a new [[Team:Paris_Saclay/Experiments#ligation with pJet\|protocol]] which uses pJET as cloning vector.
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	A heat shock transformation was made on the cloning samples using the following [[Team:Paris_Saclay/Experiments#heat-shocktransformation\|protocol]]		A heat shock transformation was made on the cloning samples using the following [[Team:Paris_Saclay/Experiments#heat-shocktransformation\|protocol]]