Difference between revisions of "Team:ASIJ Tokyo"

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{{ASIJ_Tokyo}}
 
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<div class="column full_size" >
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<h2> Welcome to iGEM 2016! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
  
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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</ul>
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</div>
  
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                <div class="item active ">
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<div class="highlight">
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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</div>
  
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                    <img src="https://static.igem.org/mediawiki/2016/e/e0/T--ASIJ_Tokyo--Bike.jpg" alt="" />
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                    <div class="carousel-caption">
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<h5> Wiki template information </h5>
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                        <h1 class="slide-h1 wow bounceInDown" data-wow-duration="1s">THE BIG WONDER</h1>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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                        <h2 class="slide-h2 wow bounceInLeft" data-wow-duration="2s" data-wow-delay="1s">ULTIMATE TEMPLATE</h2>
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                    <img src="https://static.igem.org/mediawiki/2016/e/e0/T--ASIJ_Tokyo--Bike.jpg" alt="" />
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                    <div class="carousel-caption">
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                        <h1 class="slide-h1 wow bounceInDown" data-wow-duration="2s">RESPONSIVE DESIGN</h1>
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                        <h2 class="slide-h2 wow bounceInLeft" data-wow-duration="2s" data-wow-delay="1s">FREE TO USE</h2>
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                    <img src="https://static.igem.org/mediawiki/2016/e/e0/T--ASIJ_Tokyo--Bike.jpg" alt="" />
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                    <div class="carousel-caption">
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                        <h1 class="slide-h1 wow bounceInDown" data-wow-duration="2s">HANDCRAFTED </h1>
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                        <h2 class="slide-h2 wow bounceInLeft" data-wow-duration="2s" data-wow-delay="1s">ONLY FOR YOU</h2>
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                <li data-target="#carousel-example" data-slide-to="1"></li>
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    <div id="nav">
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        <ul class="list-unstyled">
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            <li><a data-toggle="tooltip" data-placement="left" title="Home" href="#home-sec">Home</a></li>
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            <li><a data-toggle="tooltip" data-placement="left" title="About" href="#about-sec">About</a></li>
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            <li><a data-toggle="tooltip" data-placement="left" title="Features" href="#features-sec">Features</a></li>
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            <li><a data-toggle="tooltip" data-placement="left" title="Team" href="#team-sec">Team</a></li>
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            <li><a data-toggle="tooltip" data-placement="left" title="Contact" href="#contact-sec">Contact</a></li>
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                    <h1 data-scroll-reveal="enter from the bottom after 0.1s">Project Description </h1>
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<p>
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We are surrounded by plastic everyday, specifically PET (Polyethylene terephthalate). Considered as the most common type of polyester, PET is a plastic resin often used for packaging consumer products. We can also find it in our water bottles, plastic toys, and even in the fiber of our clothing. PET is a combination of two monomers known as ethylene glycol and purified terephthalic acid that, when combined, form the polymer PET. The problem with PET is that although it is a convenient material for humans to use, it does not easily biodegrade. As PET accumulates in the ecosystems around the world, it poses detrimental effects on habitats worldwide. That is why it is crucial that we find an efficient way to degrade PET. 
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Upon conducting our research on plastic degradation, shortly after deciding to focus on this topic, our team came across a joint collaboration research project from Keio University and the Kyoto Institute of Technology, two prestigious universities in Japan. Their paper explained their recent research on PET degradation into terephthalic acid and ethylene glycol using a new bacterium originating from Japan called Ideonella Saikaienis. We were inspired by Ideonella Saikaienis because it was a wonderful PET degrader (containing PETase) from Japan, our current home. Given this, when we realized that the production of PETase was from a uniquely Japanese context, we saw it only apt to center our project around this as it showcases a special (and scientifically recent) feature of the Japanese environment. Consequently, our aim and experimental idea were born: to optimize the use of PETase and hopefully add a new biobrick to the iGEM catalog. Turkey’s 2014 project on PET degradation as well as The University of Washington's 2012 project and Darmstadt's 2012 Project also provided us with inspiration on the potential applications for our project in addition to lab protocol ideas. </p>
 
  
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<p>
 
  
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We chose to optimize PETase as our goal to make a new type of biobrick available to future iGEM teams. We hope that this will make experiments in the following years easier to conduct. However, in order to even get to that stage, we recognized that we needed a well thought out procedure. Our team is currently working on this step. Thus far we have determined we will be using a western blot test to help us deal with the signal peptides. Currently we also have three promoters (one on the slightly weaker side and two on the stronger side) taken from the list of Anderson promoters to test in order to determine how to make PETase most efficient. Before testing such promoters though, we need to isolate  evaluate each promoters' effectiveness and efficiency. This information is essential before we proceed onto our next steps and think about real world applications. Questions we may ask ourselves following this first step include: “How long will the degradation take?” and “Will the byproducts of the degradation be harmful in any way to the environment or to people?” Our aim, as stated above, is to find a faster, more efficient process to degrade plastic. Answers to such questions will be essential in seeking solutions to global environmental problems. In regards to the first question, to our current knowledge, 450 years is the timeframe for the natural biodegradation of PET. From our perspective this is much too inefficient when you consider how much PET we use daily. As a result, our experiment will focus on increasing the efficiency of the degradation of plastic through the manipulation of PETase. These are just some examples of the types of questions that we must first consider. Once our confidence in our research progresses, we can then fully focus on individual procedural steps of our project such as the Western Blot.</p>
 
  
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
  
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</div>
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<h2>
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References</h2>
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<p>
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Keio University and Kyoto Institute of Technology. (2016, March 30). Discovery of a Bacterium that
 
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Degrades and Assimilates Poly(ethylene terephthalate) could Serve as a Degradation and/or
 
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Fermentation Platform for Biological Recycling of PET Waste Products [Press release]. Keio University. Retrieved June 30, 2016, from <p>
 
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<a href="https://www.keio.ac.jp/en/press_releases/2016/cb96u90000005501-att/160330_2.pdfp">https://www.keio.ac.jp/en/press_releases/2016/cb96u90000005501-att/160330_2.pdf</a></p>
 
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<br><p>
 
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P. (2015). FAQs - Frequently Asked Questions. Retrieved June 30, 2016, from </p>
 
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<p>
 
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<a href="http://www.petresin.org/faq.asp"> http://www.petresin.org/faq.asp</a></p>
 
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<br><p>Hampson, M. (2016, March 09). Science: Newly Identified Bacteria Break Down Tough Plastic. Retrieved
 
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June 30, 2016, from http://www.aaas.org/news/science-newly-identified-bacteria-break-down-tough-plastic
 
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<br>How Long does it take to Decompose - Facts Analysis. (2012, January 24). Retrieved June 30, 2016,
 
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from</p><p><a href="http://www.hoaxorfact.com/Science/how-long-does-it-take-to-decompose.html"> http://www.hoaxorfact.com/Science/how-long-does-it-take-to-decompose.html</a></p>
 
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<br><p>T. (n.d.). Labjournal Metabolism. Retrieved June 30, 2016, from </p>
 
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<p><a href="https://2012.igem.org/Team:TU_Darmstadt/Labjournal/Metabolism"> https://2012.igem.org/Team:TU_Darmstadt/Labjournal/Metabolism</a></p>
 
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<br><p>M. (n.d.). Team:METU Turkey project. Retrieved June 30, 2016, from </p>
 
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<p><a href="https://2014.igem.org/Team:METU_Turkey_project"> https://2014.igem.org/Team:METU_Turkey_project</a></p>
 
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<br><p>What is PET? (2015). Retrieved June 30, 2016, from </p><p><a href="http://www.napcor.com/PET/whatispet.html"> http://www.napcor.com/PET/whatispet.html</a></p>
 
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<br><p>Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maehada, Y., . . . Oda, K. (2016,
 
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March 11). Supplementary Materials for a Bacterium that Degrades and Assimilates
 
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Poly(ethylene terephthalate). Science, 351, 1196th ser. Retrieved June 30, 2016, from </p><p>file:///Users/lisawatanuki/Downloads/PETase supplemental (2).pdf</p>
 
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<p>Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., . . . Oda, K. (2016, March 11).
 
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A Bacterium that Degrades and Assimilates Poly(ethylene terephthalate). Science, 351(6278),
 
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1196-1199. doi:10.1126/science.aad6359</p>
 
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</p>
 
  
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>  
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</div>
  
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                    <h1 data-scroll-reveal="enter from the bottom after 0.1s">FEATURES </h1>
 
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<h5>Inspiration</h5>
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                <div class="col-lg-4 col-md-4 col-sm-4  col-xs-12 text-center" data-scroll-reveal="enter from the bottom after 0.5s">
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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                    <i class="fa fa-recycle fa-5x"></i>
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<ul>
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                    <h4 >Responsive Design</h4>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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                    <p>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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                    <h4>Easy to Customize</h4>
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                    <i class="fa fa-history fa-5x"></i>
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                    <h4>Free to Use</h4>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
  
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<div class="button_click" onClick=" parent.location= 'https://2016.igem.org/Special:Upload '">
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UPLOAD FILES
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                    <h1 data-scroll-reveal="enter from the bottom after 0.1s">TEAM </h1>
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                    <img src="assets/img/1t.jpg" class="img-circle img-responsive set-img" />
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                    <h2>LEENA CHI MAXI</h2>
 
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                    <h2>JHON DEO ALEX</h2>
 
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                    <h1 data-scroll-reveal="enter from the bottom after 0.1s">CONTACT </h1>
 
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                    <h2>Our Location</h2>
 
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                        234/89 , UGCH BUILDING HOUSE,
 
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                        DOUBLE LANE, NEW YORK CITY,
 
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                        UNITED STATES
 
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Revision as of 02:44, 23 August 2016

Welcome to iGEM 2016!

Your team has been approved and you are ready to start the iGEM season!

Before you start:

Please read the following pages:

Styling your wiki

You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.

While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.

Wiki template information

We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!

Editing your wiki

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Click here to edit this page!

Tips

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

  • State your accomplishments! Tell people what you have achieved from the start.
  • Be clear about what you are doing and how you plan to do this.
  • You have a global audience! Consider the different backgrounds that your users come from.
  • Make sure information is easy to find; nothing should be more than 3 clicks away.
  • Avoid using very small fonts and low contrast colors; information should be easy to read.
  • Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2016 calendar
  • Have lots of fun!
Inspiration

You can also view other team wikis for inspiration! Here are some examples:

Uploading pictures and files

You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to Team:YourOfficialTeamName/NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)

UPLOAD FILES