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<div class="container"> | <div class="container"> | ||
− | <h1 style="text-align:center"> | + | <h1 style="text-align:center">Collaborations</h1> |
<p id = "pp">Newcastle were doing an experiment which involved creating biological electronic components and asked our team | <p id = "pp">Newcastle were doing an experiment which involved creating biological electronic components and asked our team | ||
− | if | + | if we could help them by finding the thermal conductivity of growth media. With the help of our resident Biophysist |
− | <a href="#">Ryan | + | <a href="#">Ryan Edgington</a> |
we came up with a plan to measure the conductivity</p> | we came up with a plan to measure the conductivity</p> | ||
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thermocouple in the centre to measure the sample temperature. We also ran 0.65m of thin plastic coated copper wire | thermocouple in the centre to measure the sample temperature. We also ran 0.65m of thin plastic coated copper wire | ||
(0.05m^2 in diameter) through the falcon tube | (0.05m^2 in diameter) through the falcon tube | ||
− | and | + | and attached a thermocouple. This wire was hooked up to a current source which provided around 5 Amps. |
This wire was our heat source used to provide a temperature gradient. </p> | This wire was our heat source used to provide a temperature gradient. </p> | ||
<img src="https://static.igem.org/mediawiki/2016/8/88/T--Exeter--Home_collab_cond.jpg" style="float:right; width:40vw; height:60vh;"> | <img src="https://static.igem.org/mediawiki/2016/8/88/T--Exeter--Home_collab_cond.jpg" style="float:right; width:40vw; height:60vh;"> | ||
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the temperature difference for 10 minutes a test and this produced the graph on the right. | the temperature difference for 10 minutes a test and this produced the graph on the right. | ||
− | Once this difference was recorded The first 50 seconds of data were extracted and the process | + | Once this difference was recorded The first 50 seconds of data were extracted and the process repeated. |
− | We did this at least 5 times per sample to obtain a more | + | We did this at least 5 times per sample to obtain a more reliable result. </p> |
<p id = "pp"> From the data we found the difference in temperature | <p id = "pp"> From the data we found the difference in temperature | ||
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at time $\alpha$ and $T(t_{2})-T(t_{1})$ is our gradient. </p> | at time $\alpha$ and $T(t_{2})-T(t_{1})$ is our gradient. </p> | ||
− | <p id = "pp"> As we were using plastic coated copper wire we had to | + | <p id = "pp"> As we were using plastic coated copper wire we had to adjust the result to find the correct value. To find the |
− | + | adjustment we set up the experiment to calculate the conductivity of water. Using the standard conductivity from | |
<a href="http://www2.bren.ucsb.edu/~dturney/WebResources_13/WaterSteamIceProperties/PropOfWaterFrom0to100Celcius.pdf | <a href="http://www2.bren.ucsb.edu/~dturney/WebResources_13/WaterSteamIceProperties/PropOfWaterFrom0to100Celcius.pdf | ||
">here</a> We found that the conductivity value was roughly | ">here</a> We found that the conductivity value was roughly | ||
one fourth of the known value at that temperature so we could this correction to find our result for lb and m9 growth media. | one fourth of the known value at that temperature so we could this correction to find our result for lb and m9 growth media. | ||
</p> | </p> | ||
− | <p id = "pp">We | + | <p id = "pp">We repeated the measurement for the lb 6 times, and for the m9 sample 5 times due to time constraints. |
Our value was taken from the average conductivity from all 6/5 tests. | Our value was taken from the average conductivity from all 6/5 tests. | ||
Each testing session had its own water calibration taken. Our errors were taken as the standard deviation of the | Each testing session had its own water calibration taken. Our errors were taken as the standard deviation of the | ||
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We found the conductivity of LB to be (605 $\pm$ 20) $\frac{mW}{Km}\text{ }$. | We found the conductivity of LB to be (605 $\pm$ 20) $\frac{mW}{Km}\text{ }$. | ||
We found the conductivity of m9 to be (570 $\pm$ 30) $\frac{mW}{Km}\text{ }$.</p> | We found the conductivity of m9 to be (570 $\pm$ 30) $\frac{mW}{Km}\text{ }$.</p> | ||
− | <p id = "pp"> These values were fairly reliable to what we | + | <p id = "pp"> These values were fairly reliable to what we experienced during the test. The lb media |
− | took the same time to cool down to | + | took the same time to cool down to equilibrium as the water control test and thus the conductivities should be the same. |
− | The m9 sample took a while to cool down so it made | + | The m9 sample took a while to cool down so it made sense that the conductivity was lower.</p> |
</div> | </div> | ||
Revision as of 13:38, 25 August 2016