The aim of our project is to create a prototype panel that can characterize the composition in the “sense of taste” receptors for each patient. In the future we can be able to conduct a genetic modification as needed or requested.

For each subject, we performed two operations:

First Operation: Includes a unique characterization of the composition of taste receptor alleles for the five different tastes. This part will be carried out for each subject. Characterization will be carried out using Taqman technology with the FLUIDIGM system.

Second Operation: Identification of the selected allele for a specific taste receptor encoded and sliced in vitro using the Cas9 RNA-guided enzyme. This change will allow us to control and navigate the ability of the sense of taste as needed.

The basic assumption of the project is based on the fact that there is a direct link between particular taste affection and the number of receptors for that taste. The ability to control the polymorphism of particular taste receptors, while leaving the others intact, may be useful to human society in many ways. For example, as a specific diet aid by reducing the ability to feel the a specific taste, reducing alcohol consumption by changing sensitivity to alcohol, reducing the risk of high blood pressure by eliminating the need for salt taste, changing the sensitivity for bitterness is what will allow swallowing a pill for the purpose of providing medical care and more ..

Design 1: Preliminary stage in the development of a panel which identifies unique alleles of taste receptors

We engineered 16 selected SNPs in favor of this reaction. Each of the SNP’s that were created enables identification of a certain taste out of the five known senses of taste, and each of the tastes will change the variability in the number of alleles that make it possible to identify the taste. The SNP’s as ordered: