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| − | {{:Team:Valencia_UPV/Templates:headerUPV}}
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| − | <html>
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| − | <body>
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| − | <!--fonts-->
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| − | <link href='https://fonts.googleapis.com/css?family=Josefin+Sans' rel=
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| − | 'stylesheet' type='text/css'><!--css-->
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| − | <link href=
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| − | "https://2016.igem.org/Team:Valencia_UPV/homecss?action=raw&ctype=text/css"
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| − | rel="stylesheet"><!--javascript-->
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| − | <script src=
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| − | "https://ajax.googleapis.com/ajax/libs/jquery/3.0.0/jquery.min.js">
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| − | </script>
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| − | <script src=
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| − | "https://2016.igem.org/Team:Valencia_UPV/homejs?action=raw&ctype=text/javascript">
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| − | </script>
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| − | <div class="container-fluid" id="mainCointainer0">
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| − | <div class="row" id="sec_0">
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| − | <div class="col-md-6">
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| − | <video controls="" id="mainVideo" width="100%"><source src=
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| − | "https://static.igem.org/mediawiki/2016/d/da/T--Valencia_UPV--presentation.mp4"
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| − | type="video/mp4"> Your browser does not support HTML5
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| − | video.</video>
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| − | </div>
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| − | <div class="col-md-6" id="rightCol" style="margin-top:-1em">
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| − | <div class="row" style="margin-right:1em"><img id=
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| − | "hype_logo_temp" src=
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| − | "https://static.igem.org/mediawiki/2016/7/77/ValenciaUPV_HYPElogo.png"></div>
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| − | <div class="row" style="margin-right:1em">
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| − | <p id="absp">Covering food necessities is mandatory, but
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| − | resources are not sustainably exploited. Global strategies
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| − | to increase food productivity and quality need to be
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| − | concealed with a local perspective, providing breeders with
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| − | the necessary technology to improve varieties. The aim of
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| − | HYPE-IT is to decrease current technological barriers for
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| − | breeding local crops using precision genome engineering,
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| − | easing the gene editing process using SynBio-inspired
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| − | simplified CRISPR/Cas9 tools. HYPE-IT brings along a
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| − | software tool that associates crop traits with specific
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| − | gene targets and designs optimal gRNAs for those targets.
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| − | HYPE-IT also incorporates a modular gene circuit that
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| − | serves as an in vivo gRNA testing system, ensuring
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| − | appropriate gRNA choice even when no precise sequence
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| − | information of local varieties is available. We aim to
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| − | develop a split-Cas9 system based on viral vectors to
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| − | efficiently deliver the editing machinery into the plant,
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| − | and to create an affordable Labcase with the necessary
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| − | laboratory equipment for HYPE-IT.</p>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="row" id="sec_1">
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| − | <div class="accordion">
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| − | <ul>
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| − | <li>
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| − | <div class="col-md-3" id="db_col">
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| − | <div class="row expand_top_row"></div>
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| − | <div class="sImg row" id="img_db" style=
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| − | " width: 100%; height: 60%;">
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| − | <img src=
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| − | "https://static.igem.org/mediawiki/2016/0/04/T--Valencia_UPV--softwareIcon.png">
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| − | <h2 class="expand_title">Data Processing
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| − | Software</h2>
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| − | <p class="expandp">We have created a genomic
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| − | data processing software. This tool allows the
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| − | user to choose the type of plant and phenotypic
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| − | variation of interest. The software will find
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| − | the gene associated with that phenotype, and
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| − | then it will give as output the optimal guide
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| − | RNA that will be used with Cas9 for knock-out
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| − | the gene. Open source will be the main
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| − | philosophy of this software, so anyone can
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| − | contribute with new parameters or new
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| − | phenotype-gene links.</p>
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| − | <a class="btn btn-default btn_home" href="#" role="button">Link</a>
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| − | </div>
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| − | </div>
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| − | </li>
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| − | <li>
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| − | <div class="col-md-3" id="ts_col">
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| − | <div class="row expand_top_row"></div>
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| − | <div class="sImg row" id="img_db" style=
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| − | " width: 100%; height: 60%;">
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| − | <img src=
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| − | "https://static.igem.org/mediawiki/2016/0/0c/T--Valencia_UPV--grnaIcon.png">
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| − | <h2 class="expand_title">gRNA Testing System</h2>
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| − | <p>The plant breeder could test the obtained guide
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| − | RNA directly in his plant, but it would take a long
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| − | time just to check if it works or not. To avoid
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| − | this, we have designed a gRNA Testing System which
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| − | does this in a fast and simple way. Using Nicotiana
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| − | benthamiana, Agrobacterium infection system, a
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| − | short sequence of the gene of his plant and a
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| − | luciferase assay, the users will surely know if the
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| − | guide RNA works on his own variety. This system
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| − | works as a switch that will show luciferase if the
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| − | gene has been knocked-out.</p>
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| − | <a class="btn btn-default btn_home" href="#" role="button">Read More</a>
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| − | </div>
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| − | </div>
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| − | </li>
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| − | <li>
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| − | <div class="col-md-3" id="vv_col">
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| − | <div class="row expand_top_row"></div>
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| − | <div class="sImg row" id="img_db" style=
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| − | " width: 100%; height: 60%;">
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| − | <img src=
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| − | "https://static.igem.org/mediawiki/2016/1/17/T--Valencia_UPV--virusIcon.png">
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| − | <h2 class="expand_title">Split Cas9</h2>
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| − | <p>As a vehicle to genetically modify the plant,
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| − | the Agrobacterium infection system is inefficient,
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| − | long and difficult. Thus, we decide to use a viral
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| − | system to enhance plant infection. Since the viral
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| − | vectors are small, the Cas9 and CPF1 endonuclease -
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| − | necessary for editing - need to be splitted in two
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| − | parts. To reassemble them inside the plant cells,
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| − | we will use intein proteins.</p>
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| − | <a class="btn btn-default btn_home" href="#" role="button">Read More</a>
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| − | </div>
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| − | </div>
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| − | </li>
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| − | <li>
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| − | <div class="col-md-3" id="lc_col">
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| − | <div class="row expand_top_row"></div>
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| − | <div class="sImg row" id="img_db" style=
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| − | " width: 100%; height: 60%;">
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| − | <img src=
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| − | "https://static.igem.org/mediawiki/2016/2/23/T--Valencia_UPV--labcaseIcon.png">
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| − | <h2 class="expand_title">Lab-Case</h2>
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| − | <p>We want to make plant gene editing accessible
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| − | for all potential users, even if they do not have
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| − | an advanced laboratory. To achieve this goal, we
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| − | have made a Lab-case, a hardware set up which
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| − | contains all the laboratory equipment and the
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| − | necessary reagents required to perform genome
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| − | editing, with simple protocols. This guarantees
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| − | availability for agricultural cooperatives,
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| − | start-ups, garage laboratories... This will be the
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| − | future of agriculture: making genome editing of
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| − | plants accessible to everyone.</p>
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| − | <a class="btn btn-default btn_home" href="#" role="button">Read More</a>
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| − | </div>
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| − | </div>
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| − | </li>
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| − | </ul>
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| − | </div>
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| − | </div>
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| − | </body>
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| − | </html>
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| − |
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| − | {{:Team:Valencia_UPV/Templates:footerUPV}}
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