Difference between revisions of "Tracks/Measurement/Interlab study/Flow Cytometry Overview"

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<li>Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for. </li>
 
<li>Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for. </li>
 
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<b><i>All teams using a flow cytometer to measure GFP must finish the following items to fulfill the InterLab:</b></i>
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<ol list="1">
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<li>Email your completed Excel file to <i>measurement (AT) igem (DOT) org</i> [note: the empty template file is provided below]</li>
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<li>Fill in the <b>Flow Cytometer Form</b> provided at the bottom of this page</li>
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</ol>
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<br>
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[[Excel File Template]]
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</p>
  
 
<b>Important!</b> If you have used a flow cytometer to measure GFP for the InterLab, please fill out the following Google Form to the best of your ability:
 
<b>Important!</b> If you have used a flow cytometer to measure GFP for the InterLab, please fill out the following Google Form to the best of your ability:

Revision as of 19:49, 26 April 2016

This page is under active development and is in draft form.





Measurement with Flow Cytometry

This is a general guide for setting up your cells for flow cytometry readings in absolute units. These steps and questions are meant to provide a general protocol and we ask that you follow them to the best of your ability.

In order to carry out this measurement protocol, you will need:

  • A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP. This channel will typically have a 488nm laser an a 530/30 filter, but the details may vary.
  • Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for.


    • All teams using a flow cytometer to measure GFP must finish the following items to fulfill the InterLab:
    1. Email your completed Excel file to measurement (AT) igem (DOT) org [note: the empty template file is provided below]
    2. Fill in the Flow Cytometer Form provided at the bottom of this page

    [[Excel File Template]]

    Important! If you have used a flow cytometer to measure GFP for the InterLab, please fill out the following Google Form to the best of your ability:

    Flow Cytometer Form