Difference between revisions of "Team:CLSB-UK/Basic Part"

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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts.  
 
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts.  
 
All cyanobacteria produce energy via photosynthesis. To do this they must import CO2 from outside the cell and one of the most important proteins in this process is CmpA.
 
All cyanobacteria produce energy via photosynthesis. To do this they must import CO2 from outside the cell and one of the most important proteins in this process is CmpA.
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CmpA forms the first part of the CmpABCD operon, coding for proteins that allow dissolved Carbon Dioxide to be transported into cyanobacteria for use in photosynthesis. The diagram shows how a lipid region in the phospholipid bilayer anchors CmpA, and the binding cleft is shown. CmpB is an integral channel protein, and CmpC and D are ATPases to drive the active transport, as well as CmpC controlling the transport rate.
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CmpA forms the first part of the CmpABCD operon, coding for proteins that allow dissolved Carbon Dioxide to be transported into cyanobacteria for use in photosynthesis. The diagram shows how a lipid region in the phospholipid bilayer anchors CmpA, and the binding cleft is shown. CmpB is an integral channel protein, and CmpC and D are ATPases to drive the active transport, as well as CmpC controlling the transport rate.</p>
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The operon is part of the CO2 concentrating mechanism (CCM) that allows CO2 to be concentrated up to 1000 times around Rubisco (an essential enzyme which catalyses a reaction with CO2 in photosynthesis and is inhibited by oxygen), which became necessary for the continuing photosynthesis of the bacteria after the Great Oxidation Event. As cyanobacteria are water dwelling, CO2 is transported in its dissolved form – H2CO3 or HCO3-  depending on the pH. This is converted to CO2 in the carboxysome.
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CmpA has the highest affinity to both the bicarbonate ion and carbonic acid of any protein in the cyanobacteria genome, and must have a wide range of pH over which it is active in order to bind to both. Below is a ribbon diagram showing CmpA at pH 5.0 on the left and 8.0 on the right. The bicarbonate ion is shown on the left in the entrance to the cleft as a sphere. The carbonic acid is shown on the right deep inside the cleft as a sphere. Carbonic acid can only bind in the presence of Ca2+ ion, which may act as a cofactor and is also shown as a sphere on the right.
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We plan to overexpress the gene coding for CmpA, found in Synechocystis PCC 6803. This would mean the bacteria take in more CO¬2 so photosynthesise more which means that there are more electrons available for use in BPVs.</p>
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Revision as of 15:48, 11 September 2016

BioPhotovoltaics

★ ALERT!

This page is used by the judges to evaluate your team for the basic part special prize.

Delete this box in order to be evaluated for this medal. See more information at Instructions for Pages for awards.

A basic part is a functional unit of DNA that cannot be subdivided into smaller component parts. BBa_R0051 is an example of a basic part, a promoter regulated by lambda cl.

Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are many opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. All cyanobacteria produce energy via photosynthesis. To do this they must import CO2 from outside the cell and one of the most important proteins in this process is CmpA. CmpA forms the first part of the CmpABCD operon, coding for proteins that allow dissolved Carbon Dioxide to be transported into cyanobacteria for use in photosynthesis. The diagram shows how a lipid region in the phospholipid bilayer anchors CmpA, and the binding cleft is shown. CmpB is an integral channel protein, and CmpC and D are ATPases to drive the active transport, as well as CmpC controlling the transport rate.

Note

This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.