Difference between revisions of "Team:Toronto/Testing"

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<html>
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Notebook
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<script type="text/javascript" src="https://2016.igem.org/Template:Toronto/Javascript?action=raw&ctype=text/javascript"></script>
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-include items in the LAB SECTION ONLY
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</html>
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-include images
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-don’t include tables
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-don’t include morning/afternoon headings
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-point form, simplify if possible
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-use proper html language:
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<h3> for months
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<h4> for weeks
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<h5> for dates
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<ul> unordered lists for all procedures
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<li> procedures are list items
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Remember to </close> all your items!
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<h3> June </h3>
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<h4> Week One </h4>
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<h5> 07/06/16 </h5>
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<ul>
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<li> Made 2 x 400mL 75% EtOH </li>
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<li> Made 30 x 1mL Chloramphenicol (CAM) stocks </li>
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<li> Created in silico primers for regular linear backbone </li>
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</ul>
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<h5> 08/06/16 </h5>
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<ul>
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<li> Prepared and autoclaved LB broth </li>
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<li> Prepared SOC media </li>
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</ul>
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<h5> 09/06/16 </h5>
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<ul>
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<li> Autoclaved SOC media and aliquoted 30 x 1mL stocks </li>
 +
<li> Obtained DH10β cells and made 5mL and 100mL overnight cultures </li>
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</ul>
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<h5> 10/06/16 </h5>
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<ul>
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<li> Made TSS buffer </li>
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<li> Grew cells to OD600 of 0.34 </li>
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<li> Made aliquots of chemically competent cells </li>
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<li> Created small nickel plasmids </li>
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</ul>
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<h4> Week Two </h4>
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<h5> 13/06/16 </h5>
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<ul>
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<li> LB prep
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<li> Autoclaved 1.5mL microcentrifuge tubes </li>
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<li> Carried out iGEM transformation efficiency protocol </li>
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<li> Plated 17 plates with inoculating loops: </li>
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<ul>
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<li> 3 x 0.5pg/uL </li>
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<li> 3 x 5pg/uL </li>
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<li> 3 x 10pg/uL </li>
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</ul>
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</ul>

Revision as of 21:34, 14 September 2016

Notebook -include items in the LAB SECTION ONLY -include images -don’t include tables -don’t include morning/afternoon headings -point form, simplify if possible -use proper html language:

for months

for weeks

for dates
    unordered lists for all procedures
  • procedures are list items Remember to </close> all your items! <h3> June </h3> <h4> Week One </h4>
    07/06/16
    • Made 2 x 400mL 75% EtOH
    • Made 30 x 1mL Chloramphenicol (CAM) stocks
    • Created in silico primers for regular linear backbone
    08/06/16
    • Prepared and autoclaved LB broth
    • Prepared SOC media
    09/06/16
    • Autoclaved SOC media and aliquoted 30 x 1mL stocks
    • Obtained DH10β cells and made 5mL and 100mL overnight cultures
    10/06/16
    • Made TSS buffer
    • Grew cells to OD600 of 0.34
    • Made aliquots of chemically competent cells
    • Created small nickel plasmids
    <h4> Week Two
13/06/16
  • LB prep
  • Autoclaved 1.5mL microcentrifuge tubes
  • Carried out iGEM transformation efficiency protocol
  • Plated 17 plates with inoculating loops:
    • 3 x 0.5pg/uL
    • 3 x 5pg/uL
    • 3 x 10pg/uL