Difference between revisions of "Team:Aix-Marseille/Integrated Practices/Process"
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Once the platinum is leached, we need to recover it. If leaching (by biological or chemical methods) is clearly recommended to improves solubilization of metals, it does not improves the concentrations. As we worked with synthetic biology, we decided to use what it is commonly employed by cells to catch metals, i.e. siderophore. Siderophores are well known to catch iron most of all but some of them have an affinity with others metals as platinum. So in our process, we planned to work with such a siderophore, called [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|Desferrioxamine B]]. This one is already employed to recover platinum in mines, and have shown high capacity to recover platinum from ores <ref>Bau and al., 2015 http://dx.doi.org.gate1.inist.fr/10.1016/j.hydromet.2015.01.002</ref>. | Once the platinum is leached, we need to recover it. If leaching (by biological or chemical methods) is clearly recommended to improves solubilization of metals, it does not improves the concentrations. As we worked with synthetic biology, we decided to use what it is commonly employed by cells to catch metals, i.e. siderophore. Siderophores are well known to catch iron most of all but some of them have an affinity with others metals as platinum. So in our process, we planned to work with such a siderophore, called [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|Desferrioxamine B]]. This one is already employed to recover platinum in mines, and have shown high capacity to recover platinum from ores <ref>Bau and al., 2015 http://dx.doi.org.gate1.inist.fr/10.1016/j.hydromet.2015.01.002</ref>. | ||
| − | Recovery yields with [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] can reach 75% of the total platinum if both of these conditions are fully respected: a lowering pH level leaching step should be performed, as well as a alcanization of medium (until a pH range between 8 and 9) before addition of siderophore. The last step devoted to rise up the pH level will be realized using a standard buffer, e.g. a Tris buffer. | + | Recovery yields with [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] can reach '''75%''' of the total platinum if both of these conditions are fully respected: a lowering pH level leaching step should be performed, as well as a alcanization of medium (until a pH range between 8 and 9) just before addition of siderophore. The last step devoted to rise up the pH level will be realized using a standard buffer, e.g. a Tris buffer. |
So where is the innovation in this step? Firstly, in our case, [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] won't be applied on the same materials where is commonly used, in our cases not ores but a leachate of ashes. Basically the main difference will be the metal concentration. | So where is the innovation in this step? Firstly, in our case, [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] won't be applied on the same materials where is commonly used, in our cases not ores but a leachate of ashes. Basically the main difference will be the metal concentration. | ||
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''For instance....; CALCULS POUR 1G COMBIEN PRODUIT UNE ECOLI DE DFHOB,,???'' | ''For instance....; CALCULS POUR 1G COMBIEN PRODUIT UNE ECOLI DE DFHOB,,???'' | ||
| − | '''To sum up, this step consist in adding an appropriated quantity of Siderophore producer E. coli to the leachate.''' | + | '''To sum up, this step consist in adding an appropriated quantity of Siderophore producer E. coli to the leachate.''' |
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==Siderophore producer lysis== | ==Siderophore producer lysis== | ||
