Difference between revisions of "Team:TEC-Costa Rica/Project/Safety"

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<a style="color: #e0e0d1;" href="https://2016.igem.org/Team:TEC-Costa_Rica/Project">Project</a>
 
<a style="color: #e0e0d1;" href="https://2016.igem.org/Team:TEC-Costa_Rica/Project">Project</a>
 
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<a style="color: #e0e0d1;" href="https://2016.igem.org/Team:TEC-Costa_Rica/Human_Practice">Human Practice</a>
 
<a style="color: #e0e0d1;" href="https://2016.igem.org/Team:TEC-Costa_Rica/Human_Practice">Human Practice</a>
 
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<div class="jumbotron text-center">    
 
<div class="jumbotron text-center">    
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<h1>SynBioThon 2016: rewriting the code</h1>
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<h1>Safety</h1>
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<div class="concept">
 
<div class="concept">
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<p style="text-align: center;">SynBioThon 2016 (SBT16) is a Synthetic Biology tournament, in which teams of students work for a weekend to create a genetic device that can solve some kind of problem.</p>
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<p style="text-align: center;">Our project is related to the detection of a human disease via a biomarker molecule found in the urine of patients with prostate cancer. Since local regulation does not allow us to work with human samples, we designed our project in a way that it can work as a proof-of-concept of a detection system and pose no risk to human health.</p>
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</div>
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<h2>Lab work</h2>
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<p>During our work in the lab, we followed a series of rules to ascertain that no risks were undertaken.</p>
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    <div style="width: 25%" class="col-sm-1">
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      <span class="glyphicon glyphicon-heart logo-small"></span>
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      <p>Use of nitrile gloves</p>
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    <div style="width: 25%" class="col-sm-1">
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      <p>Use of safety goggles</p>
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    <div style="width: 25%" class="col-sm-1">
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      <span class="glyphicon glyphicon-leaf logo-small"></span>
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      <p>Management of bacteria in biosafety cabinet</p>
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    </div>
 
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<h2>Reach</h2>
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<br><br>
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<p>For SBT16, we had diverse talks in different classes and universities, trying to reach the most diverse participants. In our college (TEC), we spoke with people of different majors (Biotechnology Engineering, Material Science, Agricultural Engineering, and others). We also went to two other public universities: University of Costa Rica (UCR), where we spoke with people of Biology, Microbiology and Pharmacy, and the National University (UNA), where wetalked to students of Biology. We also gave talks in private universities such as UCIMED (Medical School), LEAD University (Business Management), and others.</p>
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<p><br>Prior to the event, we had preparatory workshops with students so they could get a grasp of genetics, molecular biology, and bioinformatics if they weren’t familiar with them. We had three workshops at UCR, one at UNA, and four at TEC. We had obout 80 students overall in a full-day session. We had a theoric section where we explained the basics of genetics and molecular biology, and then an activity to test the concepts, and after lunch a demonstrative session of bioinformatics, centered in databases and benchling, which was followed by work.</p>
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      <p>Usage of volatile chemicals in fume hood</p>
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      <span class="glyphicon glyphicon-wrench logo-small"></span>
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      <h2>Projects</h2>
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      <p>Cleaning and Sterilization of the Lab</p>
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      <p style="text-align: center;"><a style="color: black;" class="btn btn-default synbiothon-details" href="https://2016.igem.org/Team:TEC-Costa_Rica/Human_Practice/Synbiothon/Projects" role="button">View details &raquo;</a></p>
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      <p>Correct disposal of bio-hazardous residues</p>
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      <h2>Mentors and Judges</h2>
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      <p>Separate areas for work with bacteria, DNA and electrophoresis</p>
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      <h2>Evaluation</h2>
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<h2>The winners</h2>
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<h2>Project safety</h2>
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<p>In order to have a functional system without having to use human samples, we decided to synthesize a DNA fragment of the gene we are going to detect, so we can express it in the bacteria. Then, we are working with dCas9, inteins, TEV protease, and GFP proteins, which are not from pathogenic organisms and were obtained from the Distribution or as gBlocks. All the experiments and cloning is made in Escherichia coli strains DH5alpha, TOP10 and BL21. No pathogenic organisms are used in the lab.</p>
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Revision as of 20:21, 26 September 2016

Safety

Our project is related to the detection of a human disease via a biomarker molecule found in the urine of patients with prostate cancer. Since local regulation does not allow us to work with human samples, we designed our project in a way that it can work as a proof-of-concept of a detection system and pose no risk to human health.

Lab work

During our work in the lab, we followed a series of rules to ascertain that no risks were undertaken.

Use of lab coat

Use of nitrile gloves

Use of safety goggles

Management of bacteria in biosafety cabinet



Usage of volatile chemicals in fume hood

Cleaning and Sterilization of the Lab

Correct disposal of bio-hazardous residues

Separate areas for work with bacteria, DNA and electrophoresis

Project safety

In order to have a functional system without having to use human samples, we decided to synthesize a DNA fragment of the gene we are going to detect, so we can express it in the bacteria. Then, we are working with dCas9, inteins, TEV protease, and GFP proteins, which are not from pathogenic organisms and were obtained from the Distribution or as gBlocks. All the experiments and cloning is made in Escherichia coli strains DH5alpha, TOP10 and BL21. No pathogenic organisms are used in the lab.

iGEM TEC Costa Rica

Tecnológico de Costa Rica
Biotechnology Investigation Center
Cartago, Costa Rica
prostaltec@gmail.com

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