Difference between revisions of "Team:Tianjin/Note/6803"

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<h1 class="entry-title"><a href="https://www.camarts.cn/archives/4252.html" title="查看《火影忍者疾风传》中的全部作品" rel="bookmark">火影忍者疾风传</a></h1>
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<h1 class="entry-title"><a href="https://www.camarts.cn/archives/4252.html" title="查看《火影忍者疾风传》中的全部作品" rel="bookmark">Week7(9/26/2016-10/2/2016)</a></h1>
 
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<p>火影忍者十分钟疾风传。了解其中最佳CP<a href="https://www.camarts.cn/archives/tag/%e5%b8%83%e5%b0%94%e6%b4%a5" title="查看所有与鸣樱相关的照片">鸣樱</a>从小就是青梅竹马。没有月光，除了远处县城依稀的灯光和偶尔驶过的车灯外，便是一片漆黑，伸手不见五指，只能在满天繁星的映衬下依稀看见些随风而动的树影。而这，正是拍摄银河的最佳时机。<br />
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<li>Plasmids pT-13-19-15 were handed in for sequencing, which confirmed its correctness.</li>
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<b>The sequencing results for them were correct.</b>
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<a href="https://www.camarts.cn/archives/4252.html"><img class="aligncenter size-full wp-image-4256" src="http://static.camarts.cn/images/spinner-1600.gif"  alt="IMG_2956" width="800" height="533" srcset="images/img_1.jpg 800w, http://img.camarts.cn/2016/01/IMG_2956-150x100.jpg 150w, http://img.camarts.cn/2016/01/IMG_2956-300x200.jpg 300w, http://img.camarts.cn/2016/01/IMG_2956-768x512.jpg 768w, http://img.camarts.cn/2016/01/IMG_2956-450x300.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /><noscript><img class="aligncenter size-full wp-image-4256" src="http://img.camarts.cn/2016/01/IMG_2956.jpg?imageView/2/w/800/h/800/q/90" alt="IMG_2956" width="800" height="533" srcset="http://img.camarts.cn/2016/01/IMG_2956.jpg 800w, http://img.camarts.cn/2016/01/IMG_2956-150x100.jpg 150w, http://img.camarts.cn/2016/01/IMG_2956-300x200.jpg 300w, http://img.camarts.cn/2016/01/IMG_2956-768x512.jpg 768w, http://img.camarts.cn/2016/01/IMG_2956-450x300.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /></noscript></a></p>
 
<a href="https://www.camarts.cn/archives/4252.html"><img class="aligncenter size-full wp-image-4256" src="http://static.camarts.cn/images/spinner-1600.gif"  alt="IMG_2956" width="800" height="533" srcset="images/img_1.jpg 800w, http://img.camarts.cn/2016/01/IMG_2956-150x100.jpg 150w, http://img.camarts.cn/2016/01/IMG_2956-300x200.jpg 300w, http://img.camarts.cn/2016/01/IMG_2956-768x512.jpg 768w, http://img.camarts.cn/2016/01/IMG_2956-450x300.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /><noscript><img class="aligncenter size-full wp-image-4256" src="http://img.camarts.cn/2016/01/IMG_2956.jpg?imageView/2/w/800/h/800/q/90" alt="IMG_2956" width="800" height="533" srcset="http://img.camarts.cn/2016/01/IMG_2956.jpg 800w, http://img.camarts.cn/2016/01/IMG_2956-150x100.jpg 150w, http://img.camarts.cn/2016/01/IMG_2956-300x200.jpg 300w, http://img.camarts.cn/2016/01/IMG_2956-768x512.jpg 768w, http://img.camarts.cn/2016/01/IMG_2956-450x300.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /></noscript></a></p>
 
<p> <a href="https://www.camarts.cn/archives/4252.html" class="more-link">查看Team Tianjin全部实验 <span class="meta-nav">&rsaquo;</span></a></p>
 
<p> <a href="https://www.camarts.cn/archives/4252.html" class="more-link">查看Team Tianjin全部实验 <span class="meta-nav">&rsaquo;</span></a></p>
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<h1 class="entry-title"><a href="https://www.camarts.cn/archives/4235.html" title="查看《喀纳斯印象》中的全部作品" rel="bookmark">火影忍者疾风传</a></h1>
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<p>终于来到喀纳斯——中国最西北的角落，此次西北之行可到达的最远处。在这个纬度高达 48 度的地方，山坡上的植被更加茂密；太阳下落得很慢，阳光以一个非常低的角度横扫过来，跨过群山，透过树木，在地上都投出长长的影子，非常好看。<br />
 
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<a href="https://www.camarts.cn/archives/4235.html"><img class="aligncenter size-full wp-image-4239" src="http://static.camarts.cn/images/spinner-1600.gif" srcset="images/1_16.jpg 800w, images/1_16.jpg 150w, images/1_16.jpg 300w, images/1_16.jpg 768w, images/1_16.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /><noscript><img class="aligncenter size-full wp-image-4239" src="http://img.camarts.cn/2016/01/IMG_2889.jpg?imageView/2/w/800/h/800/q/90" alt="IMG_2889" width="800" height="533" srcset="http://img.camarts.cn/2016/01/IMG_2889.jpg 800w, http://img.camarts.cn/2016/01/IMG_2889-150x100.jpg 150w, http://img.camarts.cn/2016/01/IMG_2889-300x200.jpg 300w, http://img.camarts.cn/2016/01/IMG_2889-768x512.jpg 768w, http://img.camarts.cn/2016/01/IMG_2889-450x300.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /></noscript></a></p>
 
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<span class="sep">发表于 </span>2016 年 1 月 20 日<span class="sep"> | </span>
 
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<span class="entry-utility-prep entry-utility-prep-cat-links">发表在</span> <a href="https://www.camarts.cn/archives/category/%e8%a5%bf%e5%8c%97%e8%a1%8c" rel="category tag">漩涡鸣人</a> </span>
 
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<span class="entry-utility-prep entry-utility-prep-tag-links">标签有</span> <a href="https://www.camarts.cn/archives/tag/%e5%86%b2%e4%b9%8e%e5%b0%94" rel="tag">春野樱</a>、<a href="https://www.camarts.cn/archives/tag/%e5%b8%83%e5%b0%94%e6%b4%a5" rel="tag">漩涡鸣人</a>、<a href="https://www.camarts.cn/archives/tag/xinjiang" rel="tag">新疆</a>、<a href="https://www.camarts.cn/archives/tag/%e9%98%bf%e5%8b%92%e6%b3%b0" rel="tag">火影</a> </span>
 
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<h1 class="entry-title"><a href="https://www.camarts.cn/archives/4222.html" title="查看《冲乎尔牧场》中的全部作品" rel="bookmark">火影忍者十分钟疾风传</a></h1>
 
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<p>告别<a href="https://www.camarts.cn/archives/4193.html">禾木乡</a>，继续向喀纳斯方向前行，途中经过<a href="https://www.camarts.cn/archives/tag/%e5%86%b2%e4%b9%8e%e5%b0%94" title="查看所有与 冲乎尔 相关的照片">冲乎尔</a>乡。这里是一个天然牧场，坐落在小盆地，群山环抱，乡民主要以放牧为生。果然，不时就能看见牧羊人赶着几百头“<a href="https://www.camarts.cn/archives/tag/%e9%98%bf%e5%8b%92%e6%b3%b0" title="查看所有与 阿勒泰 相关的照片">阿勒泰</a>大尾羊”在公路上浩浩荡荡地走着，一路扬起滚滚烟尘，甚是壮观。<br />
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<a href="https://www.camarts.cn/archives/4222.html"><img class="aligncenter size-full wp-image-4229" src="http://static.camarts.cn/images/spinner-1600.gif" data-original="images/1_17.jpg" alt="IMG_2861" width="800" height="533" srcset="images/1_17.jpg 800w, images/1_17.jpg 150w, images/1_17.jpg 300w, images/1_17.jpg 768w, images/1_17.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /><noscript><img class="aligncenter size-full wp-image-4229" src="http://img.camarts.cn/2016/01/IMG_2861.jpg?imageView/2/w/800/h/800/q/90" alt="IMG_2861" width="800" height="533" srcset="http://img.camarts.cn/2016/01/IMG_2861.jpg 800w, http://img.camarts.cn/2016/01/IMG_2861-150x100.jpg 150w, http://img.camarts.cn/2016/01/IMG_2861-300x200.jpg 300w, http://img.camarts.cn/2016/01/IMG_2861-768x512.jpg 768w, http://img.camarts.cn/2016/01/IMG_2861-450x300.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /></noscript></a></p>
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<p> <a href="https://www.camarts.cn/archives/4222.html" class="more-link">查看本辑全部作品 <span class="meta-nav">&rsaquo;</span></a></p>
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<span class="sep">发表于 </span>2016 年 1 月 10 日<span class="sep"> | </span>
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发表在<a href="https://www.camarts.cn/archives/category/%e8%a5%bf%e5%8c%97%e8%a1%8c" rel="category tag">西北行</a> </span>
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<span class="entry-utility-prep entry-utility-prep-tag-links">标签有</span> <a href="https://www.camarts.cn/archives/tag/%e5%86%b2%e4%b9%8e%e5%b0%94" rel="tag">春野樱</a>、<a href="https://www.camarts.cn/archives/tag/%e5%b8%83%e5%b0%94%e6%b4%a5" rel="tag">漩涡鸣人</a>、<a href="https://www.camarts.cn/archives/tag/xinjiang" rel="tag"火影</a>、<a href="https://www.camarts.cn/archives/tag/%e9%98%bf%e5%8b%92%e6%b3%b0" rel="tag">鸣樱</a> </span>
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<h1 class="entry-title"><a href="https://www.camarts.cn/archives/4193.html" title="查看《禾木掠影》中的全部作品" rel="bookmark">忍者之路</a></h1>
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<p>一路北行，来到<a href="https://www.camarts.cn/archives/tag/%e7%a6%be%e6%9c%a8" title="查看所有与 禾木 相关的照片">禾木</a>乡。这里几乎是中国的最西北角，与哈萨克斯坦、俄罗斯和蒙古等三国接壤。禾木地广人稀，和我去过的许多边境小城很像。穿行而过，不见几户人家，除了几个正在建设的小旅馆外，能零星看到的只是几个小木屋和蒙古包。<br />
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<a href="https://www.camarts.cn/archives/4193.html"><img class="aligncenter size-full wp-image-4202" src="http://static.camarts.cn/images/spinner-1600.gif" data-original="images/1_18.jpg" alt="IMG_2779" width="800" height="533" srcset="images/1_18.jpg 800w, images/1_18.jpg 150w, images/1_18.jpg 300w, images/1_18.jpg 768w, images/1_18.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /><noscript><img class="aligncenter size-full wp-image-4202" src="images/1_18.jpg" alt="IMG_2779" width="800" height="533" srcset="http://img.camarts.cn/2015/12/IMG_2779.jpg 800w, images/1_18.jpg 150w, images/1_18.jpg 300w, images/1_18.jpg 768w, images/1_18.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /></noscript></a></p>
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<span class="sep">发表于 </span>2015 年 12 月 30 日<span class="sep"> | </span>
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发表在</span> <a href="https://www.camarts.cn/archives/category/%e8%a5%bf%e5%8c%97%e8%a1%8c" rel="category tag">春野樱</a> </span>
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<span class="entry-utility-prep entry-utility-prep-tag-links">标签有</span> <a href="https://www.camarts.cn/archives/tag/%e5%b8%83%e5%b0%94%e6%b4%a5" rel="tag">春野樱</a>、<a href="https://www.camarts.cn/archives/tag/xinjiang" rel="tag">新疆</a>、<a href="https://www.camarts.cn/archives/tag/%e7%a6%be%e6%9c%a8" rel="tag">漩涡鸣人</a>、<a href="https://www.camarts.cn/archives/tag/%e9%98%bf%e5%8b%92%e6%b3%b0" rel="tag">阿勒泰</a> </span>
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<h1 class="entry-title"><a href="https://www.camarts.cn/archives/4176.html" title="查看《北疆树林》中的全部作品" rel="bookmark">北疆树林</a></h1>
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<p>继续在<a href="https://www.camarts.cn/archives/tag/%e9%93%81%e7%83%ad%e5%85%8b%e6%8f%90" title="查看所有与 铁热克提 相关的照片">铁热克提</a>附近沿着蜿蜒的小道行摄，不知不觉间便绕到这座高山的另一侧。这里的杨树长得更高耸茂密，西斜的阳光恰到好处地将树梢照得金黄透亮，一道道树影投射在绒毛地毯般的甸上，构成近乎完美的光影，令人心旷神怡，不舍离。<br />
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<a href="https://www.camarts.cn/archives/4176.html"><img class="aligncenter size-full wp-image-4177" src="http://static.camarts.cn/images/spinner-1600.gif" data-original="http://img.camarts.cn/2015/12/IMG_2696.jpg?imageView/2/w/800/h/800/q/90" alt="IMG_2696" width="800" height="533" srcset="http://img.camarts.cn/2015/12/IMG_2696.jpg 800w, http://img.camarts.cn/2015/12/IMG_2696-150x100.jpg 150w, http://img.camarts.cn/2015/12/IMG_2696-300x200.jpg 300w, http://img.camarts.cn/2015/12/IMG_2696-768x512.jpg 768w, http://img.camarts.cn/2015/12/IMG_2696-450x300.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /><noscript><img class="aligncenter size-full wp-image-4177" src="http://img.camarts.cn/2015/12/IMG_2696.jpg?imageView/2/w/800/h/800/q/90" alt="IMG_2696" width="800" height="533" srcset="http://img.camarts.cn/2015/12/IMG_2696.jpg 800w, http://img.camarts.cn/2015/12/IMG_2696-150x100.jpg 150w, http://img.camarts.cn/2015/12/IMG_2696-300x200.jpg 300w, http://img.camarts.cn/2015/12/IMG_2696-768x512.jpg 768w, http://img.camarts.cn/2015/12/IMG_2696-450x300.jpg 450w" sizes="(max-width: 800px) 100vw, 800px" /></noscript></a></p>
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<p> <a href="https://www.camarts.cn/archives/4176.html" class="more-link">查看本辑全部作品 <span class="meta-nav">&rsaquo;</span></a></p>
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<span class="sep">发表于 </span><a href="https://www.camarts.cn/archives/4176.html" title="06:00" rel="bookmark"><time class="entry-date" datetime="2015-12-23T06:00:48+00:00" pubdate>2015 年 12 月 23 日</time></a><span class="by-author"> <span class="sep"> 由 </span> <span class="author vcard"><a class="url fn n" href="https://www.camarts.cn/archives/author/dandyweng" title="查看所有由 Dandy Weng 发布的文章" rel="author">Dandy Weng</a></span></span>                <span class="sep"> | </span>
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<span class="entry-utility-prep entry-utility-prep-cat-links">发表在</span> <a href="https://www.camarts.cn/archives/category/%e8%a5%bf%e5%8c%97%e8%a1%8c" rel="category tag">西北行</a> </span>
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<span class="entry-utility-prep entry-utility-prep-tag-links">标签有</span> <a href="https://www.camarts.cn/archives/tag/%e5%93%88%e5%b7%b4%e6%b2%b3" rel="tag">哈巴河</a>、<a href="https://www.camarts.cn/archives/tag/xinjiang" rel="tag">新疆</a>、<a href="https://www.camarts.cn/archives/tag/%e9%93%81%e7%83%ad%e5%85%8b%e6%8f%90" rel="tag">铁热克提</a>、<a href="https://www.camarts.cn/archives/tag/%e9%98%bf%e5%8b%92%e6%b3%b0" rel="tag">阿勒泰</a> </span>
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<span class="total-pictures"><a href="https://www.camarts.cn/archives/4176.html">13 张照片</a></span>
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<span class="comments-link"><a href="https://www.camarts.cn/archives/4176.html#comments"><b>7</b> 条评论</a></span>
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Revision as of 15:53, 28 September 2016

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Week3(8/29/2016-9/4/2016)

  • pMV-G19
  • pMV-G15
  • Colonies were used to inoculate overnight cultures.
  • Plasmids were isolated using a miniprep kit.
    • Amplification of 19 and 15 with Q5 High-Fidelity DNA Polymerase out of E.coli
  • 19 amplification at 65.0°C with 19.rev/fwd primes.
    • PCR worked, positive control worked, no amplification of 19.
  • 15 amplification at 65.0°C with 15.rev/fwd primes.
    • PCR worked, positive control worked, no amplification of 15.
  • A product length of 900 bp was expected. The gel shows that most plasmids seem to be correct.
  • This result was confirmed by sequencing.
  • The fragments of 19 and 15 were purified with PCR Purification Kit.
    • Show More Ligation of 15 with 19
  • Fragment of 19 was phosphorylated and then purified with DNA Purification Kit.
  • 19 was ligated into T vectors via TA clone and transformed into E.coli via heat shock.
  • A colony PCR was performed with five colonies.
    • Gel electrophoresis showed that 5 colonies were positive for insertion of 19. Two of these colonies containing 19 were used to inoculate overnight cultures.
  • 15 gene fragment was phosphorylated.
  • Plasmids containing T vector with 19(pT-19)were isolated using a miniprep kit.
  • Mono-restriction digest of pT-19 with stu I.
  • The enzyme-digested product was dephosphorylation.
  • Dephosphorylated plasmid and phosphorylated gene 15 were connected.
    • Ligation product was transformed into E.coli via heat shock.
  • A colony PCR was performed with twelve colonies.
    • Gel electrophoresis showed that 2 colonies were positive for connecting the plasmid and gene fragment. These two colonies were used to inoculate overnight cultures.
  • Plasmids with correct sequence of 19-15 were isolated using a miniprep kit.
  • Mono-restriction digest of pT-19-15 with Nru I.
  • The enzyme-digested product was dephosphorylation.
    • Show More IMG_2956

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    Week4(9/5/2016-9/11/2016)

  • Colonies containing gene 13 were used to inoculate overnight cultures.
  • Plasmids were isolated using a miniprep kit.
    • Amplification of 13 with Q5 High-Fidelity DNA Polymerase out of E.coli
  • 13 amplification at 65.0°C with 13.rev/fwd primes
    • PCR worked, positive control worked, no amplification of 13
  • The fragments of 13 were purified with PCR Purification Kit.
  • 13 gene fragment was phosphorylated.
    • Show More Insertion of Ni promoter and ligation of 13-19-15
  • Ni inducible promoter was ligated into pCPC-3301 vector.
  • Phosphorylated 13 was ligated into pT-19-15 and transformed into E.coli via heat shock.
  • Single colonies were obtained by plating.
  • A colony PCR of Ni inducible promoter(pCPC-3301-Ni) was performed with 12 colonies.
    • Two of the successful ones were used to inoculate overnight cultures.
  • A colony PCR of pT-13-19-15 was performed with 7 colonies.
    • Two of the successful ones were used to inoculate overnight cultures.
  • Two kinds of plasmids were isolated using a miniprep kit.
    • Show More IMG_2956

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    Week5(9/12/2016-9/18/2016)

  • Plasmids pT-13-19-15 were handed in for sequencing, which confirmed its correctness.
    • The sequencing results for both of them were error.
  • Colonies containing Ni inducible promoter were used to inoculate overnight cultures.
  • PCR was performed to check if the gene fragments were ligated correctly.
    • 13_ fwd and 15_rev on pT-13-19-15
    Gel electrophoresis showed that it failed.
  • Plasmids pCPC-3031-Ni were isolated using a miniprep kit.
  • Several PCRs were performed to check if the gene fragments were ligated correctly.
    • 1.13_fwd and 13_rev on pT-13-19-15
    2.19_fwd and 19_rev on pT-13-19-15
    3.15_fwd and 15_rev on pT-13-19-15
    4.13_fwd and 19_rev on pT-13-19-15
    5.19_fwd and 15_rev on pT-13-19-15
    6.13_ wd and 15_rev on pT-13-19-15
    The fourth and sixth ones were not successful.
  • Repetition: Phosphorylated 13 was ligated into pT-19-15 and transformed into E.coli via heat shock.
  • Repetition: Several PCRs were performed to check if the gene fragments were ligated correctly.
    • 1.13_fwd and 13_rev on pT-13-19-15
    2.13_fwd and 19_rev on pT-13-19-15
    The second one was failed.
    IMG_2956

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    Week6(9/19/2016-9/25/2016)

  • Medium preparation :BG-11.
  • 19_ fwd and 15_rev were used to amplify 19-15.
  • Gel electrophoresis showed that amplification of fragments was successfull.
  • Ligated 13 and 19-15 via overlap PCR.
  • This PCR worked well and the products were extracted from the gel using a Agarose Gel DNA Extration Kit.
  • Restriction digest on pCPC-3031-Ni with Sac I.
  • The fragment 13-19-15 was ligated onto T vector and transformed into E.coli via heat shock.
  • A colony PCR of pT-13-19-15 was performed with 12 colonies.
    • Two of these colonies containing pT-13-19-15 were used to inoculate overnight cultures.
  • 13-19-15 gene fragment was phosphorylated.
  • Phosphorylated 13-19-15 was ligated into pCPC-3031-Ni and transformed into E.coli via heat shock.
  • Plasmids pT-13-19-15 were isolated using a miniprep kit.
  • A colony PCR of pCPC-3031-Ni-13-19-15 was performed with 12 colonies.
    • Three colonies were proved to be true. And two of them were used to inoculate overnight cultures.
    IMG_2956

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    Notice: This page is currently under construction. Contents in this page are temporaory and will be modified several times before the final release.     — 2016 iGEM Team Tianjin