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+ | <h2 class="red">Week 5th September-11th September</h2> | ||
+ | <h3>Automated 96 well plate of fabric</h3> | ||
+ | <p class=”input”> | ||
+ | We got an idea of using fluorescent 96 well plate, placing cotton circles inside the wells and gluing the tip holder(which is yellow in the images). This way there is no diffusion, cotton is trapped inside the wells, as the well's inner diameter is bigger than cotton circle diameter of 6.5mm and the circles of tip holder are less than 6.5mm. This is an arrant analog of plastic microplate we tried earlier and the pdms microplate. | ||
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+ | |||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | <img class="assay" src="https://static.igem.org/mediawiki/2016/a/a0/Paris_Bettencourt-Notebook_Assay_Template.jpg" alt=« Success" height=“150px“ /> | ||
+ | |||
+ | <img class="assay" src="https://static.igem.org/mediawiki/2016/b/b4/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview.jpg" alt=« Success" height=“150px“ /> | ||
+ | <img class="assay" src="https://static.igem.org/mediawiki/2016/1/1e/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview2.jpg" alt=« Success" height=“150px“ /> | ||
+ | </p> | ||
+ | |||
+ | <h3>Dilution test</h3> | ||
+ | <p class=”input”> | ||
+ | We did a a dilution test to see if we can clearly witness the change in the intensity of the wine of different dilutions in the Microplate. The results are terrific. We could find the difference in intensity of wells from each other and between before dilution test and after dilution test. | ||
+ | |||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | <img class="assay" src="https://static.igem.org/mediawiki/2016/7/75/Paris_Bettencourt-Notebook_Assay_Finaldesign_Dilutiontest.jpg" alt=« Success" height=“150px“ /> | ||
+ | <img class="assay" src="https://static.igem.org/mediawiki/2016/d/d6/Paris_Bettencourt-Notebook_Assay_Finaldesign_beforedilution.jpg" alt=« Success" height=“150px“ /> | ||
+ | <img class="assay" src="https://static.igem.org/mediawiki/2016/4/48/Paris_Bettencourt-Notebook_Assay_Finaldesign_afterdilution.jpg" alt=« Success" height=“150px“ /> | ||
+ | |||
+ | </p> | ||
+ | |||
</div> | </div> |
Revision as of 18:21, 30 September 2016
Week 5th September-11th September
Automated 96 well plate of fabric
We got an idea of using fluorescent 96 well plate, placing cotton circles inside the wells and gluing the tip holder(which is yellow in the images). This way there is no diffusion, cotton is trapped inside the wells, as the well's inner diameter is bigger than cotton circle diameter of 6.5mm and the circles of tip holder are less than 6.5mm. This is an arrant analog of plastic microplate we tried earlier and the pdms microplate.
Dilution test
We did a a dilution test to see if we can clearly witness the change in the intensity of the wine of different dilutions in the Microplate. The results are terrific. We could find the difference in intensity of wells from each other and between before dilution test and after dilution test.