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5. Put the tip plate holder on the top of the microplate and adhere it to the microplate using a glue. | 5. Put the tip plate holder on the top of the microplate and adhere it to the microplate using a glue. | ||
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− | 6. Cover it with a lid and the microplate is ready to use for experiments. The same can be done for other fabrics like silk, | + | 6. Cover it with a lid and the microplate is ready to use for experiments. The same can be done for other fabrics like silk, polystyrene, nylon etc. |
<br> | <br> | ||
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<img class="assay" src="https://static.igem.org/mediawiki/2016/b/b4/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview.jpg" alt=« Success" height=“150px“ /> | <img class="assay" src="https://static.igem.org/mediawiki/2016/b/b4/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview.jpg" alt=« Success" height=“150px“ /> | ||
<img class="assay" src="https://static.igem.org/mediawiki/2016/1/1e/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview2.jpg" alt=« Success" height=“150px“ /> | <img class="assay" src="https://static.igem.org/mediawiki/2016/1/1e/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview2.jpg" alt=« Success" height=“150px“ /> | ||
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<img class="assay" src="https://static.igem.org/mediawiki/2016/d/d6/Paris_Bettencourt-Notebook_Assay_Finaldesign_beforedilution.jpg" alt=« Success" height=“150px“ /> | <img class="assay" src="https://static.igem.org/mediawiki/2016/d/d6/Paris_Bettencourt-Notebook_Assay_Finaldesign_beforedilution.jpg" alt=« Success" height=“150px“ /> | ||
<img class="assay" src="https://static.igem.org/mediawiki/2016/4/48/Paris_Bettencourt-Notebook_Assay_Finaldesign_afterdilution.jpg" alt=« Success" height=“150px“ /> | <img class="assay" src="https://static.igem.org/mediawiki/2016/4/48/Paris_Bettencourt-Notebook_Assay_Finaldesign_afterdilution.jpg" alt=« Success" height=“150px“ /> | ||
− | + | </p> | |
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Revision as of 18:50, 30 September 2016
Automated and DIY microplate on fabric
An automated 96 well microplate for fabric was made using a fluorescent microplate, 96 well tip holder and laser-cut cotton circles.
1. Spread a flat, wet cotton sheet on glass substrate of 96 well plate dimension.
2. 96 Wet cotton circles of 6.5mm are laser cut on glass substrate.
3. Remove the fabric. The circles of cotton stay on the glass substrate.
4. Place the glass substrate of cotton circles on fluorescent microplate such that all circles face the wells and align well.
5. Hold it tight and move the glass substrate laterally so that all the cotton circles fall into the wells.
5. Put the tip plate holder on the top of the microplate and adhere it to the microplate using a glue.
6. Cover it with a lid and the microplate is ready to use for experiments. The same can be done for other fabrics like silk, polystyrene, nylon etc.