Difference between revisions of "Team:Alverno CA/MakingtheAgaroseGel"
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<center><p>Ingredients/Materials: *</p></center> | <center><p>Ingredients/Materials: *</p></center> | ||
| − | <p>- 0.6g agarose <img src="https://static.igem.org/mediawiki/2016/5/50/T--Alverno_CA--ProtocolPicture_Gel.png" alt="A Picture of a Gel style="width: | + | <p>- 0.6g agarose <img src="https://static.igem.org/mediawiki/2016/5/50/T--Alverno_CA--ProtocolPicture_Gel.png" alt="A Picture of a Gel style="width:200px;"></p> |
<p>- 50mL TBE 1x</p> | <p>- 50mL TBE 1x</p> | ||
<p>- 5μL SYBRsafe / 2.5μL EcoStain (light- and heat-sensitive)</p> | <p>- 5μL SYBRsafe / 2.5μL EcoStain (light- and heat-sensitive)</p> | ||
Revision as of 00:11, 6 October 2016
Experiment & Protocol
Making the Agarose Gel
*Note: Pipettes are needed.
Ingredients/Materials: *
- 0.6g agarose 
- 50mL TBE 1x
- 5μL SYBRsafe / 2.5μL EcoStain (light- and heat-sensitive)
- 250mL conical flask
- scale
- Microwave
- Gel mold
Directions:
1. Weigh 0.6g of agarose into flask.
2. Add 50mL of TBE 1x Buffer
3. Microwave agarose solution until dissolved for 1 min (take out halfway to swirl)
(If the liquid looks distorted, agarose solution need to be microwaved more)
4. Cool slightly and add SYBRsafe or EcoStain
5. Pour gel into mold and put the combs into their spots
6. Cool until solidified.
