Difference between revisions of "Team:OUC-China/Protocol"
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<div class="row"> | <div class="row"> | ||
<div class="col-md-3"></div> | <div class="col-md-3"></div> | ||
| − | <div class="col-md-6" style="margin:0 auto;max-width:80%;border:2px solid #c0c0c0;border-radius:10px;background:#fff;"> | + | <div class="col-md-6" style="margin:0 auto;max-width:80%;border:2px solid #c0c0c0;border-radius:10px;background:#fff;text-decoration:none;"> |
<h3>1. Elementary Operation</h3> | <h3>1. Elementary Operation</h3> | ||
<p> | <p> | ||
| − | 1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf | + | 1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf">Luria-Bertani liquid and Luria-Bertani solid medium</a><br> |
2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br> | 2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br> | ||
3) <a href="https://static.igem.org/mediawiki/2016/d/dc/T--OUC-China--Polymerase_Chain_Reaction.pdf">Polymerase Chain Reaction</a><br> | 3) <a href="https://static.igem.org/mediawiki/2016/d/dc/T--OUC-China--Polymerase_Chain_Reaction.pdf">Polymerase Chain Reaction</a><br> | ||
Revision as of 15:08, 8 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Overlap PCR
2) Gibson
3) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
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