Difference between revisions of "Team:Austin UTexas/Results"
| Line 8: | Line 8: | ||
<h2>Conjugation</h2> | <h2>Conjugation</h2> | ||
| − | <p>We have attempted to conjugate GFP into both <i>G. oxydans</i> and <G. hansenii> | + | <p>We have attempted to conjugate GFP into both <i>G. oxydans</i> and <i>G. hansenii</i> with a Diaminopimelic Acid (DAP) auxotrophic strain of <i> E. coli </i>. The plasmid contains the vector pMMB67EH, the promoter PA-1, GFP and a spectinomycin resistance gene. </p> |
| + | |||
| + | <p>The first conjugation was done with KOM strains 4 (<i>G. oxydans</i>) 5 (<i> G. oxydans </i> and 15 (<i> L. fermentati </i>). We attempted these conjugations before sequencing the recipient strains, so that is why we tried to conjugate into <i> L. fermentati </i>. First, a mixture between a KOM strain and the DAP auxotroph strain were plated on a LB+DAP solid medium to allow for conjugation to occur. After 24 hours of incubation, I scraped up the growth and plated each conjugation mixture onto a LB+Spec plate.</p> | ||
| + | |||
| + | [[File:T--Austin UTexas--LB+SPEC1stconjugation.jpg|thumb|LB+SPEC plates that contain conjugation mixtures of KOM 4, 5 and 15 (<i> L. fermentati </i>) | ||
| + | |||
| + | |||
</div> | </div> | ||
Revision as of 08:55, 10 October 2016
Conjugation
We have attempted to conjugate GFP into both G. oxydans and G. hansenii with a Diaminopimelic Acid (DAP) auxotrophic strain of E. coli . The plasmid contains the vector pMMB67EH, the promoter PA-1, GFP and a spectinomycin resistance gene.
The first conjugation was done with KOM strains 4 (G. oxydans) 5 ( G. oxydans and 15 ( L. fermentati ). We attempted these conjugations before sequencing the recipient strains, so that is why we tried to conjugate into L. fermentati . First, a mixture between a KOM strain and the DAP auxotroph strain were plated on a LB+DAP solid medium to allow for conjugation to occur. After 24 hours of incubation, I scraped up the growth and plated each conjugation mixture onto a LB+Spec plate.
[[File:T--Austin UTexas--LB+SPEC1stconjugation.jpg|thumb|LB+SPEC plates that contain conjugation mixtures of KOM 4, 5 and 15 ( L. fermentati )