Difference between revisions of "Team:Uppsala"

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{{Uppsala}}
 
 
{{Uppsala/CSS}}
 
{{Uppsala/CSS}}
  
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<html>
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<html lang="en">
  
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<div class="column full_size" >
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<head>
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<img src="https://static.igem.org/mediawiki/2016/4/42/Uppsala_jumbotron_header.jpeg">
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    <meta charset="utf-8" />
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</div>
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    <title> Playing with Bootstrap </title>
  
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<div class="column full_size" >
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    <link href='https://fonts.googleapis.com/css?family=Open+Sans:300,400' rel='stylesheet' type='text/css'>
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<h2> Welcome to iGEM 2016! </h2>
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    <link href='https://fonts.googleapis.com/css?family=Lato:100,300' rel='stylesheet' type='text/css'>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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    <link href="https://fonts.googleapis.com/css?family=Lora" rel="stylesheet">
  
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</div>
 
  
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<div class="column half_size" >
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</head>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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</ul>
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</div>
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<div class="column half_size" >
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<body>
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<div class="highlight">
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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</div>
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</div>
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<div class="column full_size" >
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<div class="body">
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<h5> Wiki template information </h5>
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    <header>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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        <div class="jumbotron">
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            <div class="container">
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                <div class="row">
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                    <img style="float: right" src="https://static.igem.org/mediawiki/2016/4/41/TeamUppsala_logotemp.svg" class="img-responsive" />
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                </div>
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            </div>
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        </div>
  
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</div>  
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</html>
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{{Uppsala/Navbar}}
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<html>
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    </header>
  
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    <div class="divider"></div>
  
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    <div class="container">
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        <div class="row">
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          <h2> Project overview <small>What we are aiming for</small> </h2>
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            <div class="col-lg-9">
  
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                <p>
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We are developing a method to make CRISPR and microfluidics more available to iGEM teams and researchers. The technique will be used to fuse a fluorescent protein called UnaG to a genomic protein in both prokaryotes and eukaryotes. We are including state of the art research involving the CRISPR associated protein CPF1 and microfluidic methods. </p>
  
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<div class="column half_size" >
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<p> UnaG is a fluorescent protein that needs bilirubin as a co-factor in order to fluoresce. Bilirubin binds non-covalently, which facilitates the possibility of creating inducible fluorescent switches from UnaG. Bilirubin occurs naturally in higher vertebrate cells, making it suitable as a biosensor for research on vertebrates. It is therefore convenient to use UnaG together with CRISPR systems, such as CRISPR/CPF1. </p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
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</div>
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<p> CPF1 cuts downstream of the PAM sites and leaves 5’ overhangs. By providing UnaG with the complementary overhangs, we could insert this fluorescent protein in the genome of our host. In order to increase the chances of correct insertion, we aim to engineer UnaG with homology arms, which enables cells to insert UnaG by means of homologous recombination in the exact position where the genome has been cut. </p>
  
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<p> To facilitate the insertion of the genomic material we will design a microfluidic chip capable
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of transformation. This will be done through soft lithography by 3D printing a mold and
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baking a PDMS chip on it. A microfluidic chip will reduce the amount of reagents needed to perform a transformation, which could potentially reduce the cost and workload of a conventional transformation. The chip methods are not size-dependent, therefore it will be possible to do any given plasmid insertion with the same device. </p>
  
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<div class="column half_size" >
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<p> By using this chip, cell transformation becomes simpler and cheaper to do for other iGEM teams and small laboratories. In our project we will use it along with CRISPR to fuse UnaG with a genomic protein in yeast, but a microfluidic chip could potentially be used for any transformation technique. </p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>
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</div>
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            </div>
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            </div>
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  <div class="row">
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                    <div class="col-lg-4">
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                        <a href="https://2016.igem.org/Team:Uppsala/Project/CRISPR">
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                            <div class="panel text-center">
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                                <div id="panel" class="panel-body">
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                                    <h3> CRISPR<br>CPF1 </h3>
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                                    <span> 鋏 </span>
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                                </div>
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                            </div>
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                        </a>
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                    </div>
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                    <div class="col-lg-4">
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                        <a href="https://2016.igem.org/Team:Uppsala/Project/UnaG">
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                            <div class="panel text-center">
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                                <div id="panel" class="panel-body">
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                                    <h3> UnaG </h3>
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                                    <span> 鰻 </span>
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                                </div>
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                            </div>
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                        </a>
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                    </div>
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                    <div class="col-lg-4 ">
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                        <a href="https://2016.igem.org/Team:Uppsala/Project/Microfluidics">
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                            <div class="panel text-center">
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                                <div id="panel" class="panel-body">
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                                    <h3> Micro-<br>fluidics </h3>
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                                    <span> 流 </span>
  
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<div class="column half_size" >
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                                </div>
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<h5>Inspiration</h5>
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                            </div>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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                        </a>
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<ul>
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                    </div>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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                </div>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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</div>
 
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{{Uppsala/Footer}}
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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</body>
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<div class="button_click"  onClick=" parent.location= 'https://2016.igem.org/Special:Upload '">
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UPLOAD FILES
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Revision as of 13:56, 21 June 2016

Playing with Bootstrap

Project overview What we are aiming for

We are developing a method to make CRISPR and microfluidics more available to iGEM teams and researchers. The technique will be used to fuse a fluorescent protein called UnaG to a genomic protein in both prokaryotes and eukaryotes. We are including state of the art research involving the CRISPR associated protein CPF1 and microfluidic methods.

UnaG is a fluorescent protein that needs bilirubin as a co-factor in order to fluoresce. Bilirubin binds non-covalently, which facilitates the possibility of creating inducible fluorescent switches from UnaG. Bilirubin occurs naturally in higher vertebrate cells, making it suitable as a biosensor for research on vertebrates. It is therefore convenient to use UnaG together with CRISPR systems, such as CRISPR/CPF1.

CPF1 cuts downstream of the PAM sites and leaves 5’ overhangs. By providing UnaG with the complementary overhangs, we could insert this fluorescent protein in the genome of our host. In order to increase the chances of correct insertion, we aim to engineer UnaG with homology arms, which enables cells to insert UnaG by means of homologous recombination in the exact position where the genome has been cut.

To facilitate the insertion of the genomic material we will design a microfluidic chip capable of transformation. This will be done through soft lithography by 3D printing a mold and baking a PDMS chip on it. A microfluidic chip will reduce the amount of reagents needed to perform a transformation, which could potentially reduce the cost and workload of a conventional transformation. The chip methods are not size-dependent, therefore it will be possible to do any given plasmid insertion with the same device.

By using this chip, cell transformation becomes simpler and cheaper to do for other iGEM teams and small laboratories. In our project we will use it along with CRISPR to fuse UnaG with a genomic protein in yeast, but a microfluidic chip could potentially be used for any transformation technique.

CRISPR
CPF1

鋏

UnaG

鰻

Micro-
fluidics

流