Difference between revisions of "Team:Exeter/Project"
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</div> | </div> | ||
<div class="row"> | <div class="row"> | ||
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<div class="col-xs-12"> | <div class="col-xs-12"> | ||
<img style="max-width:100%;padding: 5px 30% 5px 30%;" src="https://static.igem.org/mediawiki/2016/8/8a/T--Exeter--ministat.jpg"> | <img style="max-width:100%;padding: 5px 30% 5px 30%;" src="https://static.igem.org/mediawiki/2016/8/8a/T--Exeter--ministat.jpg"> | ||
| − | <span class="caption" style="padding: 5px 30% 5px 30%;">Ministat running a preliminary experiment to calibrate parameters such as dilution rate, temperature of the heat block. 50 ml burettes used here to accurately measure effluent levels. 1 litre Duran bottles were used for effluent collection in the main experiment due to greater volumes of effluent. </span> | + | <div class="col-xs-3"></div> |
| + | <div class="col-xs-6"><span class="caption" style="padding: 5px 30% 5px 30%;">Ministat running a preliminary experiment | ||
| + | to calibrate parameters such as dilution rate, temperature of the heat block. 50 ml burettes used here | ||
| + | to accurately measure effluent levels. 1 litre Duran bottles were used for effluent collection | ||
| + | in the main experiment due to greater volumes of effluent. </span></div> | ||
| + | <div class="col-xs-3"></div> | ||
</div> | </div> | ||
| − | |||
</div> | </div> | ||
| − | + | <p id="pp">A growth curve was performed for <i>E. coli</i> BL21 (DE3) to determine the maximum specific growth rate. | |
| + | Dilution rate is equal to maximum specific growth rate. The dilution rate of the culture was calculated by measuring | ||
| + | flow rate at a setting of 7.5 rpm on the peristaltic pump. For practical reasons the pump could not be run faster than | ||
| + | this due to the amount of media needed. The dilution rate was set at 0.2 which produced cultures that grew at average OD | ||
| + | of.... readings were taken with a Bug Lab OD scanner. When the same sample was measured in a tecan infinite 200 pro plate | ||
| + | reader the Bug Lab showed approximately a reading three times higher, however the difference between cultures was consistent. | ||
| + | </p> | ||
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<img src="https://static.igem.org/mediawiki/2016/9/9f/T--Exeter--Project_dark.jpg" | <img src="https://static.igem.org/mediawiki/2016/9/9f/T--Exeter--Project_dark.jpg" | ||
style="max-width:100%;margin:auto;display:block;"> | style="max-width:100%;margin:auto;display:block;"> | ||
| − | <span class="caption">A graph | + | <span class="caption">A graph displaying the amount of light on the control samples covered in foil inside the light chamber |
| − | the | + | compared to the light levels without the light being on.</span> |
<br> | <br> | ||
<br> | <br> | ||
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<img src="https://static.igem.org/mediawiki/2016/e/e3/T--Exeter--Project_spectra.jpg" | <img src="https://static.igem.org/mediawiki/2016/e/e3/T--Exeter--Project_spectra.jpg" | ||
style="max-width:100%;margin:auto;display:block;"> | style="max-width:100%;margin:auto;display:block;"> | ||
| − | <span class="caption"> | + | <span class="caption">The light spectra emited from our light source.</span> |
| − | + | ||
<br> | <br> | ||
<br> | <br> | ||
Revision as of 21:10, 10 October 2016
