Difference between revisions of "Team:Korea U Seoul/Safety"

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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h2>Safety</h2>
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<h5>Safe Project Design</h5>
 
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<p>Our project include expressing a set of agar degrading enzymes, and diaphorase in E.coli. We also use shewanella oneidensis MR-1. </p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<p>1, Agar degrading enzymes are highly unlikely to be dangerous. Its only function is to degrade agarose. They are expressed in strain of E,coli, BW25113, which is classified as risk group 1. </p>
  
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<p>2. Diaphorase is also highly unlikely to be dangerous. It is often used in EFCs to generate electricity. They are expressed in strain of E,coli, BL21(DE3), which is also classified as risk group 1.</p>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<p>3. Shewanella oneidensis MR-1 is a non-pathogetic species which is classified as risk group 1. It is often used in MFCs to generate electricity.
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<p>All of our parts and organisms are not dangerous. However, we do use few dangerous materials such as EtBr. When we  are handling those dangerous materials, we always use gloves for protection. We also always sterilize biological wastes by autoclaving to prevent biological materials from spreading.
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 01:54, 11 October 2016

Safety

Our project include expressing a set of agar degrading enzymes, and diaphorase in E.coli. We also use shewanella oneidensis MR-1.

1, Agar degrading enzymes are highly unlikely to be dangerous. Its only function is to degrade agarose. They are expressed in strain of E,coli, BW25113, which is classified as risk group 1.

2. Diaphorase is also highly unlikely to be dangerous. It is often used in EFCs to generate electricity. They are expressed in strain of E,coli, BL21(DE3), which is also classified as risk group 1.

3. Shewanella oneidensis MR-1 is a non-pathogetic species which is classified as risk group 1. It is often used in MFCs to generate electricity.

All of our parts and organisms are not dangerous. However, we do use few dangerous materials such as EtBr. When we are handling those dangerous materials, we always use gloves for protection. We also always sterilize biological wastes by autoclaving to prevent biological materials from spreading.