Difference between revisions of "Team:SCAU-China/Proof"

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{{SCAU-China}}
 
  
 
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<h3>★ ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">gold medal criterion for proof of concept</a>. </p>
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<link href="https://2016.igem.org/Team:SCAU-China/CSS/result?action=raw&ctype=text/css" rel="stylesheet" type="text/css">
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<div class="menu">
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  <a href="#"><img src="https://static.igem.org/mediawiki/2016/b/b2/T--SCAU-China--logo_2.png"></a>
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  <a href="#"><img src="https://static.igem.org/mediawiki/2016/e/e3/T--SCAU-China--logo_3.png"></a></div>
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  <li><a href="https://2016.igem.org/Team:SCAU-China">HOME</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Team">TEAM</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Project">PROJECT</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/PART">PART</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Result">RESULT</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/NOTEBOOK">NOTEBOOK</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/HP">HUMAN PRACTICE</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Safety">SAFETY</a></li>
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      <li><a href="#vecter" class="on">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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<h4> What should we do for our proof of concept? </h4>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/3/31/T--SCAU-China--img_1.jpg"></div>
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<p>  
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      <div class="text">Fig.1 Not I digestion of constructs cotaining different numbers of genes.
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You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
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Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue" class="on">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      <div class="img">
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      <img src="https://static.igem.org/mediawiki/2016/6/61/T--SCAU-China--img_2.jpg"><br>
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    <div class="vecter">
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      <a id="pcr"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr" class="on">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      <img src="https://static.igem.org/mediawiki/2016/0/0f/T--SCAU-China--img_5.jpg"><br>
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      <a id="phenotype"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype" class="on">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      <img src="https://static.igem.org/mediawiki/2016/9/94/T--SCAU-China--image014.jpg">&nbsp;&nbsp;
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  <img src="https://static.igem.org/mediawiki/2016/0/02/T--SCAU-China--image015.png"><br><br>
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      <img src="https://static.igem.org/mediawiki/2016/8/8a/T--SCAU-China--image020.jpg">&nbsp;&nbsp;
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    <div class="vecter">
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      <ul class="nav">
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      <a id="hplc"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc" class="on">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/d/dc/T--SCAU-China--image024.png"></div>
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      <div class="text">Fig.n HPLC analysis diagram of standard samples of astaxanthin(a);
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HPLC analysis diagram of  samples extract  from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of  samples extract  from  HG-WT rice (c);<br><br>
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Compared with the standard samples with HPLC analysis diagram, you can see the sample extract  from rice and standard samples  have the same peak at the same time, show that the  transgenic rice seed which carry the four genes  expressed astaxanthin. Results indicate  that we are successful synthesize astaxanthin by rice endosperm synthetic .</div> 
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/0/0b/T--SCAU-China--image026.png"></div>
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/4/42/T--SCAU-China--image028.png"></div>
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/8/84/T--SCAU-China--image030.png"></div>   
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="modeling"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling" class="on">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/2/2e/T--SCAU-China--image032.png"></div>
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      <div class="text">Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .</div> 
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Revision as of 09:14, 11 October 2016

Fig.1 Not I digestion of constructs cotaining different numbers of genes. Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.



     

     
Fig.n HPLC analysis diagram of standard samples of astaxanthin(a); HPLC analysis diagram of samples extract from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of samples extract from HG-WT rice (c);

Compared with the standard samples with HPLC analysis diagram, you can see the sample extract from rice and standard samples have the same peak at the same time, show that the transgenic rice seed which carry the four genes expressed astaxanthin. Results indicate that we are successful synthesize astaxanthin by rice endosperm synthetic .
Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .