Difference between revisions of "Team:NKU China/Collaborations"

As a team with relatively insufficient experience, we have been actively seeking collaboration with other iGEM teams to discuss our projects and learn from their experience. Particularly, we have worked in close cooperation with iGEM 2015 Gold Medal team TJUSLS_China. Team TJUSLS_China is an iGEM team from College of Life Science, Tianjin University and it has been a team with outstanding performance They won one single gold award and two single nominations last year. Moreover, the campus of Tianjin University is adjacent to the campus of Nankai University, which made TJUSLS_China the team that we had the closest cooperation with.

During this summer, we had several meetings to discuss our projects and share the problems that we had encountered. We had learnt a lot from each of those meetings and we believe that the projects of both parties have been promoted through the enthusiastic discussions.

Meanwhile, we have provided them with fluorescence microscopy as well as necessary training and instructions according to the requirements of their experiment. They obtained delicate pictures and further confirmed their results using the microscopy that we provided.

iGEM team Tianjin comes from School of Chemical Engineering and Technology, Tianjin University. Their project this year aims to solve the problem of polyethylene terephthalate (PET) pollution using biodegradation method by express an enzyme that is capable of efficiently degrade PET in various species of model microorganisms. Besides Escherichia coli and yeast, they also asked as for strains of Pseudomonas putida and Pseudomonas aeruginosa and corresponding expression vectors, expecting to express the PET degradation enzyme in more chassis organisms. We provided the strains and materials they asked for as well as necessary instructions.

Many iGEM teams have participated in the Interlab Study this year. Unfortunately, however, materials from the 2016 Interlab Kit such as plasmids failed to function effectively due to problems in the manufacture, transportation, storage and experiment process. Team HUST_China was encountered with such problem. Due to some unknown issues, they failed to successfully transform the plasmid for Positive Control group into Escherichia coli DH5α and they turned to us for assistance. After we had learnt their situation, we immediately cultured the Positive Control strain that we had successfully transformed and send the plasmid they had required to them. With our prompt help, team HUST_China eventually obtained the Positive Group strain.

This year is the first year that team JSNU_China participates in the iGEM Competition and we have had a close cooperation with them. We have provided them with detailed suggestions on composition of team members, selection of project focus and establishment of their team wiki etc.

Meanwhile we have also participated in the questionnaire investigation of multiple teams (listed below) and provided suggestions and helpful feedbacks to them.