Difference between revisions of "Team:Aachen/Basic Part"
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| − | <h1>Evolution of a New Synthetase</h1> | + | <h1 style="padding-left: 0.8cm;">Evolution of a New Synthetase</h1> |
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Revision as of 15:11, 11 October 2016
Parts
| BioBrick | Description |
| K2020000 | subtilisin E gene, optimized for E. coli codon usage |
| K2020001 | subtilisin E gene, optimized for E. coli codon usage, with leader sequence pelB |
| K2020002 | expression system of subtilisin E in E. coli |
| K2020003 | mutated version of expression system of subtilisin E in E. coli (S221Y) |
| K2020004 | mutated version of expression system of subtilisin E in E. coli (S221stop) |
| K2020005 | mutated version of expression system of subtilisin E in E. coli (Y-1W) |
| K2020006 | mutated version of expression system of subtilisin E in E. coli (Y-1stop) |
| K2020020 | subtilisin E gene, optimized for S. cerevisiae codon usage |
| K2020021 | subtilisin E gene, optimized for S. cerevisiae codon usage, with the leader sequence MFalpha |
| K2020022 | subtilisin E gene, optimized for S. cerevisiae codon usage, with the leader sequence MFalpha and His Tag |
| K2020023 | expression system of subtilisin E in S. cerevisiae |
| K2020024 | mutated version of expression system of subtilisin E in S. cerevisiae (S221W) |
| K2020025 | mutated version of expression system of subtilisin E in S. cerevisiae (S221stop) |
| K2020026 | leader sequence MFalpha (different version of the biobrick BBa_K792002) |
| K2020027 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (BBa_E2060) |
| K2020028 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (BBa_K165005) |
| K2020029 | leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (BBa_E2050) |
| K2020040 | screening plasmid for incorporation of non-canonical amino acids → pRXG (twin pFRY) |
| K2020041 | screening plasmid for incorporation of non-canonical amino acids → pRYG (twin pFRYC) |
| K2020042 | tRNA specific for tyrosine and UAG codon in E. coli |
| K2020043 | tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS |
| K2020044 | tRNA synthetase specific for the ncAA AminoY and UAG codon in E. coli → AminoY-RS |
| K2020045 | tRNA synthetase specific for the ncAA NitroY and UAG codon in E. coli → NitroY-RS |
| K2020046 | tRNA synthetase specific for the ncAA CNF and UAG codon in E. coli → CNF-RS |
| K2020047 | tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS |
| K2020050 | tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS |
| K2020051 | mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G |
| K2020052 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1 |
| K2020053 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2 |
| K2020054 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3 |
| K2020055 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4 |
| K2020056 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5 |
| K2020057 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6 |
| K2020058 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7 |
| K2020059 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8 |
| K2020060 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9 |
| K2020061 | tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10 |
