Difference between revisions of "Team:OUC-China/Description"

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{{OUC-China}}
 
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<li class="active"><a href="#">Home</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Project">Project</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a>
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<ul class="dropdown-menu">
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<li><a href="#">Basic part</a></li>
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<li><a href="#">Composite parts</a></li>
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<li><a href="#">Part collection</a></li>
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</ul>
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</li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Attribution<span class="caret"></span></a>
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<ul class="dropdown-menu">
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<li><a href="#">Support</a></li>
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<li><a href="#">Collaboration</a></li>
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</ul>
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</li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<span class="caret"></span></a>
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<ul class="dropdown-menu">
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<li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li>
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                                                                <li><a href="https://2016.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
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<li><a href="#">Safety</a></li>
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</ul>
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</li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Human Practice</a></li>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<p class="text-center">As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, wecan decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.</p>
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<a href="#"><img src="https://static.igem.org/mediawiki/2016/4/41/T--OUC-China--menu-icon-1.fw.png" class="img-responsive"><p class="text-center">Purpose</p></a>
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<div class="col-sm-4">
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<a href="#"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--OUC-China--menu-icon-2.fw.png" class="img-responsive"><p class="text-center">Principle</p></a>
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<div class="col-sm-4">
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<a href="#"><img src="https://static.igem.org/mediawiki/2016/0/07/T--OUC-China--menu-icon-3.fw.png" class="img-responsive"><p class="text-center">Potential</p></a>
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<a href="#"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--OUC-China--menu-icon-4.fw.png" class="img-responsive"><p class="text-center">Attrbution</p></a>
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</div>
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<div class="col-sm-4">
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<a href="https://2016.igem.org/Team:OUC-China/Team"><img src="https://static.igem.org/mediawiki/2016/a/ad/T--OUC-China--menu-icon-5.fw.png" class="img-responsive"><p class="text-center">Team</p></a>
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</div>
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<div class="col-sm-4">
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<a href="https://2016.igem.org/Team:OUC-China/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/0/04/T--OUC-China--menu-icon-6.fw.png" class="img-responsive"><p class="text-center">Human Practice</p></a>
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<h5>What should this page contain?</h5>
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</div>
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<ul>
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<div class="oucMenuBottom">
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<li> A clear and concise description of your project.</li>
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<h1 class="text-center">Let’s take a fantastic journey!</h1>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<a href="#"><p class="text-center">Check in</p></a>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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<div class="row">
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<div class="oucBanner">
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<h3 class="text-center">Cistrons Concerto</h3>
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<div class="row oucInformation">
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<div class="col-md-7">
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<div class="column full_size" >
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<b>About</b>
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<p>Thanks to:<i><img src="image/ouc-footer-1.fw.png"></i><i><img src="image/ouc-footer-2.fw.png"></i></p>
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<h5>Advice on writing your Project Description</h5>
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<p>Designed and built by @ Jasmine Chen and @ Zexin Jiao</p>
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<p>Code licensed under Apache License v2.0</p>
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<p>
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</div>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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<div class="col-md-5">
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</p>
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<b>Contact us</b>
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<p>E-mail: oucigem@163.com</p>
−
<p>
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<p>Follow us on Facebook@ iGEM OUC<i><img src="image/ouc-footer-3.fw.png"></i></p>
−
Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<p>Find us on Google Map</p>
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</p>
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<p>We are OUC-iGEM<i><img src="image/ouc-footer-4.fw.png"></i><i><img src="image/ouc-footer-5.fw.png"></i><i><img src="image/ouc-footer-6.fw.png"></i></p>
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<h5>References</h5>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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<div class="column half_size" >
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<h5>Inspiration</h5>
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−
<p>See how other teams have described and presented their projects: </p>
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−
<ul>
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−
<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
+
−
<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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−
<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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</ul>
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Revision as of 00:44, 12 October 2016

index

Abstract


As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, wecan decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.

Purpose

Principle

Potential

Attrbution

Team

Human Practice

Let’s take a fantastic journey!

Check in

Cistrons Concerto

About

Thanks to:

Designed and built by @ Jasmine Chen and @ Zexin Jiao

Code licensed under Apache License v2.0

Contact us

E-mail: oucigem@163.com

Follow us on Facebook@ iGEM OUC

Find us on Google Map

We are OUC-iGEM