Difference between revisions of "Team:Tokyo Tech/Modeling Overview"

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<p class="normal_text">To reproduce ”Snow White”, we have designed the cell-cell communication system with improved or characterized parts and collect data from exhaustive experiments.
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<p class="normal_text">To reproduce the story of ”Snow White”, we have designed the cell-cell communication system with improved or characterized parts and collected data from comprehensive experiments. Furthermore, we constructed the mathematical model in order to simulate the behavior of the whole system and to confirm the feasibility of our story. This simulation successfully contributed to give the suggestions to wet lab experiments and confirm the feasibility of our system. In addition, in order to utilize TA (Toxin-Antitoxin) system, we developed a new software in Java for adjusting the number of ACA sequences, which MazF dimer recognizes and cleaves in mRNAs.</p>
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However, since our gene circuits are so complex that we couldn’t predict whether <span style="font-style: italic;">E. coli</span> can play the story, we constructed the mathematical model and simulate them.
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This simulation successfully contributed to give the suggestions to wet lab experiments and confirm the feasibility of our system.
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Also, in order to utilize TA system, we developed the software in Java for adjusting the number of ACA sequences, which MazF dimer recognizes and cleaves in mRNAs.</p>
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Revision as of 05:11, 12 October 2016

Model

OVERVIEW

To reproduce the story of ”Snow White”, we have designed the cell-cell communication system with improved or characterized parts and collected data from comprehensive experiments. Furthermore, we constructed the mathematical model in order to simulate the behavior of the whole system and to confirm the feasibility of our story. This simulation successfully contributed to give the suggestions to wet lab experiments and confirm the feasibility of our system. In addition, in order to utilize TA (Toxin-Antitoxin) system, we developed a new software in Java for adjusting the number of ACA sequences, which MazF dimer recognizes and cleaves in mRNAs.

MATHEMATICAL MODEL

To simulate our gene circuits, we developed an ordinary differential equation model.

[Model Development]

Show expressions

隠しスペース

RESULTS OF SIMULATION

Lastly we got and verified the desirable behavior of the fluorescence by modifying and improving them. As described below, it is consistent with the development of story.

SOFTWARE

ACA Sequence: