Difference between revisions of "Team:Northwestern/07 01"

Line 22: Line 22:
 
     <h2>Agenda:</h2>
 
     <h2>Agenda:</h2>
  
−
 
+
<ul>
 +
      <li>Constructs</li>
 +
      <li>Practice presentations</li>
 +
      <li>Ligation</li>
 +
      <li>Meeting with grad students</li>
 +
    </ul>
 +
    <h2>Results:</h2>
 +
    <p>Cloning practice: Redid the gel from yesterday. This time, we didn't run the gel quite long enough and the bands didn't separate that much.</p>
 +
    <h2>Tasks:</h2>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Michelle</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Website research</li>
 +
          <li>Golden Gate preparation</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
<div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sara</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Ran the gel again</li>
 +
          <li>Emailed Bernd</li>
 +
          <li>Looked into choosing homologous regions to add onto the signaling sequences, ran into problems with annealing temp </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tasfia</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Attempted to make overhangs on SS parts, but the Tm was too high for optimal Gibson assembly with really long overhangs</li>
 +
          <li>Cloning practice</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tyler</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Finalized homology arms for split Cas9</li>
 +
          <li>Designed RTW PCR primers of tet backbone</li>
 +
          <li>Received Promega’s order of restriction</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Jordan</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Helped with gels</li>
 +
          <li>Crowdfunding got approved</li>
 +
          <li>Edited crowdfunding page</li>
 +
          <li>Presented to grad students </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Paul</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Update presentation with GFP fusions</li>
 +
          <li>Researched GFP fusions</li>
 +
          <li>Upload sfGFP to Genome Compiler </li>
 +
        </ul>
 +
      </div>
 +
    </div>
  
 
   </article>
 
   </article>

Revision as of 15:18, 12 October 2016

Notebook

Friday, July 1st

Agenda:

  • Constructs
  • Practice presentations
  • Ligation
  • Meeting with grad students

Results:

Cloning practice: Redid the gel from yesterday. This time, we didn't run the gel quite long enough and the bands didn't separate that much.

Tasks:

Michelle

  • Website research
  • Golden Gate preparation

Sara

  • Ran the gel again
  • Emailed Bernd
  • Looked into choosing homologous regions to add onto the signaling sequences, ran into problems with annealing temp

Tasfia

  • Attempted to make overhangs on SS parts, but the Tm was too high for optimal Gibson assembly with really long overhangs
  • Cloning practice

Tyler

  • Finalized homology arms for split Cas9
  • Designed RTW PCR primers of tet backbone
  • Received Promega’s order of restriction

Jordan

  • Helped with gels
  • Crowdfunding got approved
  • Edited crowdfunding page
  • Presented to grad students

Paul

  • Update presentation with GFP fusions
  • Researched GFP fusions
  • Upload sfGFP to Genome Compiler